Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16156 |
Resumo: | CNPq PROCAD/Casadinho, PAPES/Fiocruz, Brazilian Ministry of Health (PPSUS-2012)/Fundação Araucária |
| id |
CRUZ_9365c0c4ef63d8e4f9d30713eb5dc8b2 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/16156 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Kuczera, DiogoBavia, LorenaMosimann, Ana Luiza PamplonaKoishi, Andrea CristineMazzarotto, Giovanny Augusto Camacho AntevereAoki, Mateus NóbregaMansano, Ana Maria FerrariTomeleri, Ediléia InêsCosta Junior, Wilson LiutiMiranda, Milena MenegazzoSarzi, Maria LoPavanelli, Wander RogérioConchon-Costa, IveteSantos, Claudia Nunes Duarte dosBordignon, Juliano2016-10-07T19:30:47Z2016-10-07T19:30:47Z2016KUCZERA, Diogo. et al. Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil. Virol J., v. 13, n. 93, p. 1-8, 2016.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/1615610.1186/s12985-016-0548-9CNPq PROCAD/Casadinho, PAPES/Fiocruz, Brazilian Ministry of Health (PPSUS-2012)/Fundação AraucáriaFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilSecretaria Municipal de Saúde de Cambé, Cambé, PR, Brasil.Secretaria Municipal de Saúde de Cambé, Cambé, PR, Brasil.Secretaria Municipal de Saúde de Cambé, Cambé, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunoparasitologia Experimental, Londrina, PR, Brasil.Secretaria Municipal de Saúde de Cambé, Cambé, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunoparasitologia Experimental, Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunoparasitologia Experimental, Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, BrasilBackground We report the isolation and characterization of dengue virus (DENV) serotype 4 from a resident of Santa Fé, state of Paraná, South Brazil, in March 2013. This patient presented with hemorrhagic manifestations, high viral load and, interestingly, a mixed Th1/Th17 cytokine profile. Case presentation The patient presented with classical dengue symptoms, such as fever, rash, myalgia, arthralgia, and hemorrhagic manifestations including petechiae, gum bleeding and a positive tourniquet test result. A serum sample obtained 1 day after the initial appearance of clinical symptoms was positive for NS1 viral antigen, but this sample was negative for both IgM and IgG against DENV. Dengue virus infection was confirmed by isolation of the virus from C6/36 cells, and dengue virus serotyping was performed via one-step RT-PCR. The infection was confirmed to be caused by a serotype 4 dengue virus. Additionally, based on multiple alignment and phylogeny analyses of its complete genome sequence, the viral strain was classified as genotype II (termed LRV13/422). Moreover, a mixed Th1/Th17 cytokine profile was detected in the patient’s serum, and this result demonstrated significant inflammation. Biological characterization of the virus via in vitro assays comparing LRV13/422 with a laboratory-adapted reference strain of dengue virus serotype 4 (TVP/360) showed that LRV13/422 infects both vertebrate and invertebrate cell lines more efficiently than TVP/360. However, LRV13/422 was unable to inhibit type I interferon responses, as suggested by the results obtained for other dengue virus strains. Furthermore, LRV13/422 is the first completely sequenced serotype 4 dengue virus isolated in South Brazil. Conclusion The high viral load and mixed Th1/Th17 cytokine profile observed in the patient’s serum could have implications for the development of the hemorrhagic signs observed, and these potential relationships can now be further studied using suitable animal models and/or in vitro systems.engLinfa WangIsolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengue virus serotype 4Genetic characterizationCytokinesIL-6IFN-gammaIL-17ViremiaParaná StateCitocinasInterleucina-6Interleucina-17info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16156/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALIsolation of dengue virus serotype 4.pdfIsolation of dengue virus serotype 4.pdfapplication/pdf1680810https://www.arca.fiocruz.br/bitstream/icict/16156/2/Isolation%20of%20dengue%20virus%20serotype%204.pdf125f64e2a031400408ebc58720d766d6MD52TEXTIsolation of dengue virus serotype 4.pdf.txtIsolation of dengue virus serotype 4.pdf.txtExtracted texttext/plain34336https://www.arca.fiocruz.br/bitstream/icict/16156/3/Isolation%20of%20dengue%20virus%20serotype%204.pdf.txt3ae2e36654ea0d24f92067b991d65ea7MD53icict/161562018-04-02 07:58:39.707oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-02T10:58:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| title |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| spellingShingle |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil Kuczera, Diogo Dengue virus serotype 4 Genetic characterization Cytokines IL-6 IFN-gamma IL-17 Viremia Paraná State Citocinas Interleucina-6 Interleucina-17 |
| title_short |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| title_full |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| title_fullStr |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| title_full_unstemmed |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| title_sort |
Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil |
| author |
Kuczera, Diogo |
| author_facet |
Kuczera, Diogo Bavia, Lorena Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Mazzarotto, Giovanny Augusto Camacho Antevere Aoki, Mateus Nóbrega Mansano, Ana Maria Ferrari Tomeleri, Ediléia Inês Costa Junior, Wilson Liuti Miranda, Milena Menegazzo Sarzi, Maria Lo Pavanelli, Wander Rogério Conchon-Costa, Ivete Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| author_role |
author |
| author2 |
Bavia, Lorena Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Mazzarotto, Giovanny Augusto Camacho Antevere Aoki, Mateus Nóbrega Mansano, Ana Maria Ferrari Tomeleri, Ediléia Inês Costa Junior, Wilson Liuti Miranda, Milena Menegazzo Sarzi, Maria Lo Pavanelli, Wander Rogério Conchon-Costa, Ivete Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kuczera, Diogo Bavia, Lorena Mosimann, Ana Luiza Pamplona Koishi, Andrea Cristine Mazzarotto, Giovanny Augusto Camacho Antevere Aoki, Mateus Nóbrega Mansano, Ana Maria Ferrari Tomeleri, Ediléia Inês Costa Junior, Wilson Liuti Miranda, Milena Menegazzo Sarzi, Maria Lo Pavanelli, Wander Rogério Conchon-Costa, Ivete Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| dc.subject.en.En.fl_str_mv |
Dengue virus serotype 4 Genetic characterization Cytokines IL-6 IFN-gamma IL-17 Viremia Paraná State |
| topic |
Dengue virus serotype 4 Genetic characterization Cytokines IL-6 IFN-gamma IL-17 Viremia Paraná State Citocinas Interleucina-6 Interleucina-17 |
| dc.subject.decs.pt_BR.fl_str_mv |
Citocinas Interleucina-6 Interleucina-17 |
| description |
CNPq PROCAD/Casadinho, PAPES/Fiocruz, Brazilian Ministry of Health (PPSUS-2012)/Fundação Araucária |
| publishDate |
2016 |
| dc.date.accessioned.fl_str_mv |
2016-10-07T19:30:47Z |
| dc.date.available.fl_str_mv |
2016-10-07T19:30:47Z |
| dc.date.issued.fl_str_mv |
2016 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
KUCZERA, Diogo. et al. Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil. Virol J., v. 13, n. 93, p. 1-8, 2016. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/16156 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1743-422X |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s12985-016-0548-9 |
| identifier_str_mv |
KUCZERA, Diogo. et al. Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil. Virol J., v. 13, n. 93, p. 1-8, 2016. 1743-422X 10.1186/s12985-016-0548-9 |
| url |
https://www.arca.fiocruz.br/handle/icict/16156 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Linfa Wang |
| publisher.none.fl_str_mv |
Linfa Wang |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/16156/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/16156/2/Isolation%20of%20dengue%20virus%20serotype%204.pdf https://www.arca.fiocruz.br/bitstream/icict/16156/3/Isolation%20of%20dengue%20virus%20serotype%204.pdf.txt |
| bitstream.checksum.fl_str_mv |
0271fb68963c017aa933c7aff60379f4 125f64e2a031400408ebc58720d766d6 3ae2e36654ea0d24f92067b991d65ea7 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009313944305664 |