Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil

Detalhes bibliográficos
Autor(a) principal: Santos, Luciane Amorim
Data de Publicação: 2011
Outros Autores: Cunha, Joana Paixão Monteiro, Araujo, Adriano Fernando da Silva, Alves, Carlos Roberto Brites, Castro Filho, Bernardo Galvão, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7298
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Santos, Luciane AmorimCunha, Joana Paixão MonteiroAraujo, Adriano Fernando da SilvaAlves, Carlos Roberto BritesCastro Filho, Bernardo GalvãoAlcantara, Luiz Carlos Júnior2014-02-07T17:04:54Z2014-02-07T17:04:54Z2011SANTOS, L. A. et al. Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil. Journal of Medical Virology, v. 83, n. 12, p. 2066–2072, 2011.1096-907110.1002/jmv.22170https://www.arca.fiocruz.br/handle/icict/7298engWiley Periodicals, Inc.Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Bahia Foundation for Science Development Bahia. School of Medicine and Public Health. HTLV Center / Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Bahia Foundation for Science Development Bahia. School of Medicine and Public Health. HTLV Center / Salvador, BA, Brasil / National Institutes of Health. National Cancer Institute. Bethesda, Maryland, USAThe extraordinary genetic diversity and immune evasion of human immunodeficiency virus (HIV) pose significant challenges for vaccine development and antiretroviral therapy efficacy. The objective of this study was to characterize the molecular profile of HIV-1 epidemic in Salvador, Bahia, Brazil, determining the genetic subtypes and the presence of antiretroviral resistance mutations. HIV-1 pol DNA sequences from 57 individuals infected with HIV were obtained by PCR, followed by sequencing. The subtypes were determined by phylogenetic analyses and the intersubtype recombination was investigated by bootscanning. The pol subtypes were compared with gag and env subtypes. Antiretroviral susceptibility was evaluated through the Stanford HIV resistance Database. The subtypes frequencies were: 77.2% of subtype B, 1.8% of subtype F1, and 21.0% of BF recombinant forms. Two intergenic and three intragenic BF recombinant patterns were observed. Six (10.5%) viruses were related to CRF28/CRF29, two were related to CRF12 (3.5%), and one (1.8%) was CRF39. Fourteen (24.6%) strains carried one or more mutations associated with at least intermediate resistance: 24.6% had resistance to nucleoside reverse transcriptase inhibitors, 21.0% to non-nucleoside reverse transcriptase inhibitors, and 7% to protease inhibitors. The substitutions I54V (7.0%), M184V (14.0%), and K103N (10.5%) were the most frequent within each class of drugs. The results show a high diversity of BF genotypes and a lower prevalence of major reverse transcriptase and protease drug resistance mutations in Salvador, compared with other regions of Brazil. These findings may contribute to improve treatment strategies of patients infected with HIV-1 from this Brazilian region.HIV-1CRFResistanceVariação GenéticaInfecções por HIV/epidemiologiaInfecções por HIV/virologiaHIV-1/classificaçãoHIV-1/genéticaRecombinação GenéticaAdolescenteAdultoCriançaAnálise por ConglomeradosFarmacorresistência ViralFemininoGenótipoHIV-1/efeitos de drogasHIV-1/isolamento & purificaçãoDados de Sequência MolecularMutação de Sentido IncorretoFilogeniaRNA Viral/genéticaAnálise de Sequência de DNAProdutos do Gene pol do Vírus da Imunodeficiência Humana/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSantos, LA. et al. 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dc.title.pt_BR.fl_str_mv Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
title Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
spellingShingle Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
Santos, Luciane Amorim
HIV-1
CRF
Resistance
Variação Genética
Infecções por HIV/epidemiologia
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/genética
Recombinação Genética
Adolescente
Adulto
Criança
Análise por Conglomerados
Farmacorresistência Viral
Feminino
Genótipo
HIV-1/efeitos de drogas
HIV-1/isolamento & purificação
Dados de Sequência Molecular
Mutação de Sentido Incorreto
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
title_short Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
title_full Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
title_fullStr Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
title_full_unstemmed Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
title_sort Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil
author Santos, Luciane Amorim
author_facet Santos, Luciane Amorim
Cunha, Joana Paixão Monteiro
Araujo, Adriano Fernando da Silva
Alves, Carlos Roberto Brites
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author_role author
author2 Cunha, Joana Paixão Monteiro
Araujo, Adriano Fernando da Silva
Alves, Carlos Roberto Brites
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Luciane Amorim
Cunha, Joana Paixão Monteiro
Araujo, Adriano Fernando da Silva
Alves, Carlos Roberto Brites
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
dc.subject.en.pt_BR.fl_str_mv HIV-1
CRF
Resistance
topic HIV-1
CRF
Resistance
Variação Genética
Infecções por HIV/epidemiologia
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/genética
Recombinação Genética
Adolescente
Adulto
Criança
Análise por Conglomerados
Farmacorresistência Viral
Feminino
Genótipo
HIV-1/efeitos de drogas
HIV-1/isolamento & purificação
Dados de Sequência Molecular
Mutação de Sentido Incorreto
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
dc.subject.decs.pt_BR.fl_str_mv Variação Genética
Infecções por HIV/epidemiologia
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/genética
Recombinação Genética
Adolescente
Adulto
Criança
Análise por Conglomerados
Farmacorresistência Viral
Feminino
Genótipo
HIV-1/efeitos de drogas
HIV-1/isolamento & purificação
Dados de Sequência Molecular
Mutação de Sentido Incorreto
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-02-07T17:04:54Z
dc.date.available.fl_str_mv 2014-02-07T17:04:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, L. A. et al. Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil. Journal of Medical Virology, v. 83, n. 12, p. 2066–2072, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7298
dc.identifier.issn.none.fl_str_mv 1096-9071
10.1002/jmv.22170
identifier_str_mv SANTOS, L. A. et al. Detection of distinct human immunodeficiency virus type 1 circulating recombinant forms in northeast Brazil. Journal of Medical Virology, v. 83, n. 12, p. 2066–2072, 2011.
1096-9071
10.1002/jmv.22170
url https://www.arca.fiocruz.br/handle/icict/7298
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley Periodicals, Inc.
publisher.none.fl_str_mv Wiley Periodicals, Inc.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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