Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/741 |
Resumo: | We evaluated, for the first time in Latin America, the performance of a commercial enzyme immunoassay (EIA) (Calypte Biomedical Corporation, Berkeley, Calif.) that detects human immunodeficiency virus type 1 (HIV-1)-specific antibodies in urine in comparison to standard serological assays (two commercial EIAs and a commercial Western blot [WB] assay). Paired serum and urine specimens were collected from two different groups of Brazilian patients: 225 drug users with unknown HIV status who attended drug treatment centers in Rio de Janeiro, Brazil, and 135 subjects with known HIV status. Patients showing positive results in the serum EIAs and/or in the urine EIA were serologically confirmed by WB assay. For 135 individuals with known HIV status, the urine EIA showed 100% sensitivity (74 positive samples) and 95.1% specificity (58 of 61 negative specimens). For 225 drug users, the test showed 100% sensitivity (2 positive samples) and 98.7% specificity (220 of 223 negative samples) compared to WB-confirmed serological EIA results. Thus, in a total of 360 samples, the urine EIA correctly identified all 76 HIV-positive samples and 278 of 284 negative samples (100% sensitivity and 97.9% specificity). Detailed analysis of the urine EIA results indicates that an increase of the recommended cutoff value might raise the specificity of the assay without affecting its sensitivity. Our results suggest that the HIV-1 urine EIA is a good screening test suitable for developing countries like Brazil. However, as for all other HIV screening tests on the market, it is not specific enough to be used as a one-step test and therefore requires confirmation. |
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Oelemann, Walter Martin RolandLowndes, Catherine M.Costa, Giovani C. Veríssimo daMorgado, Mariza GonçalvesCastello Branco, Luiz Roberto RibeiroGrinsztejn, BeatrizAlary, MichelBastos, Francisco Inácio Pinkusfeld Monteiro2010-08-23T16:58:44Z2010-11-04T14:19:50Z2010-11-04T14:19:50Z2010-08-23T16:58:44Z2002OELEMANN, W. M. R. et al. Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study. Journal of Clinical Microbiology, Washington, v. 40, n. 3, p. 881–885, Mar. 2002.1098-660Xhttps://www.arca.fiocruz.br/handle/icict/741We evaluated, for the first time in Latin America, the performance of a commercial enzyme immunoassay (EIA) (Calypte Biomedical Corporation, Berkeley, Calif.) that detects human immunodeficiency virus type 1 (HIV-1)-specific antibodies in urine in comparison to standard serological assays (two commercial EIAs and a commercial Western blot [WB] assay). Paired serum and urine specimens were collected from two different groups of Brazilian patients: 225 drug users with unknown HIV status who attended drug treatment centers in Rio de Janeiro, Brazil, and 135 subjects with known HIV status. Patients showing positive results in the serum EIAs and/or in the urine EIA were serologically confirmed by WB assay. For 135 individuals with known HIV status, the urine EIA showed 100% sensitivity (74 positive samples) and 95.1% specificity (58 of 61 negative specimens). For 225 drug users, the test showed 100% sensitivity (2 positive samples) and 98.7% specificity (220 of 223 negative samples) compared to WB-confirmed serological EIA results. Thus, in a total of 360 samples, the urine EIA correctly identified all 76 HIV-positive samples and 278 of 284 negative samples (100% sensitivity and 97.9% specificity). Detailed analysis of the urine EIA results indicates that an increase of the recommended cutoff value might raise the specificity of the assay without affecting its sensitivity. Our results suggest that the HIV-1 urine EIA is a good screening test suitable for developing countries like Brazil. However, as for all other HIV screening tests on the market, it is not specific enough to be used as a one-step test and therefore requires confirmation.We thank Maria Inez Linhares de Carvalho, the staff of the Ambulatório da Providência, and the staffs of the Evandro Chagas Hospital and the drug treatment centers for their unrestricted cooperation. Diagnostic kits for HIV-1 detection in urine were provided by Calypte Biomedical Corporation. Financial support for this study was provided by the Brazilian Ministry of Health and the Brazilian National Research Council (CNPq), and the FIOCRUZ AIDS program (PIAF). M.A. was the recipient of a research scholarship from Fonds de Recherche en Santé de Québec (no. 970097). W.M.R.O., M.G.M, and F.I.B. are recipients of research scholarships from CNPq.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Université Laval. Hôpital du Saint-Sacrement du CHA. Groupe de Recherche en Épidémiologie. Quebec, Canada.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Université Laval. Hôpital du Saint-Sacrement du CHA. Groupe de Recherche en Épidémiologie. Quebec, Canada.Fundação Oswaldo Cruz. Centro de Informação Científica e Tecnológica. Departamento de Informações em Saúde. Rio de Janeiro, RJ, Brasil.engAmerican Society for MicrobiologyDiagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTBASTOS_Diagnostic Detection of HIV Type_2002.pdf.txtBASTOS_Diagnostic Detection of HIV Type_2002.pdf.txtExtracted texttext/plain28852https://www.arca.fiocruz.br/bitstream/icict/741/6/BASTOS_Diagnostic%20Detection%20of%20HIV%20Type_2002.pdf.txt22c58eb07b8ed7b614f9a13d5b637353MD56ORIGINALBASTOS_Diagnostic Detection of HIV Type_2002.pdfapplication/pdf73259https://www.arca.fiocruz.br/bitstream/icict/741/2/BASTOS_Diagnostic%20Detection%20of%20HIV%20Type_2002.pdfd21f7008d627d711ef14b62a3b076a1fMD52LICENSElicense.txttext/plain1841https://www.arca.fiocruz.br/bitstream/icict/741/3/license.txt6d0d51962ce8ede313a4e08f10f64069MD53THUMBNAILBASTOS_Diagnostic Detection of HIV Type_2002.pdf.jpgBASTOS_Diagnostic Detection of HIV Type_2002.pdf.jpgGenerated Thumbnailimage/jpeg2509https://www.arca.fiocruz.br/bitstream/icict/741/5/BASTOS_Diagnostic%20Detection%20of%20HIV%20Type_2002.pdf.jpg27349948b8fbd151bf252a59dfc21c9eMD55icict/7412023-09-04 11:17:48.878oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:17:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
title |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
spellingShingle |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study Oelemann, Walter Martin Roland |
title_short |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
title_full |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
title_fullStr |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
title_full_unstemmed |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
title_sort |
Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study |
author |
Oelemann, Walter Martin Roland |
author_facet |
Oelemann, Walter Martin Roland Lowndes, Catherine M. Costa, Giovani C. Veríssimo da Morgado, Mariza Gonçalves Castello Branco, Luiz Roberto Ribeiro Grinsztejn, Beatriz Alary, Michel Bastos, Francisco Inácio Pinkusfeld Monteiro |
author_role |
author |
author2 |
Lowndes, Catherine M. Costa, Giovani C. Veríssimo da Morgado, Mariza Gonçalves Castello Branco, Luiz Roberto Ribeiro Grinsztejn, Beatriz Alary, Michel Bastos, Francisco Inácio Pinkusfeld Monteiro |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oelemann, Walter Martin Roland Lowndes, Catherine M. Costa, Giovani C. Veríssimo da Morgado, Mariza Gonçalves Castello Branco, Luiz Roberto Ribeiro Grinsztejn, Beatriz Alary, Michel Bastos, Francisco Inácio Pinkusfeld Monteiro |
description |
We evaluated, for the first time in Latin America, the performance of a commercial enzyme immunoassay (EIA) (Calypte Biomedical Corporation, Berkeley, Calif.) that detects human immunodeficiency virus type 1 (HIV-1)-specific antibodies in urine in comparison to standard serological assays (two commercial EIAs and a commercial Western blot [WB] assay). Paired serum and urine specimens were collected from two different groups of Brazilian patients: 225 drug users with unknown HIV status who attended drug treatment centers in Rio de Janeiro, Brazil, and 135 subjects with known HIV status. Patients showing positive results in the serum EIAs and/or in the urine EIA were serologically confirmed by WB assay. For 135 individuals with known HIV status, the urine EIA showed 100% sensitivity (74 positive samples) and 95.1% specificity (58 of 61 negative specimens). For 225 drug users, the test showed 100% sensitivity (2 positive samples) and 98.7% specificity (220 of 223 negative samples) compared to WB-confirmed serological EIA results. Thus, in a total of 360 samples, the urine EIA correctly identified all 76 HIV-positive samples and 278 of 284 negative samples (100% sensitivity and 97.9% specificity). Detailed analysis of the urine EIA results indicates that an increase of the recommended cutoff value might raise the specificity of the assay without affecting its sensitivity. Our results suggest that the HIV-1 urine EIA is a good screening test suitable for developing countries like Brazil. However, as for all other HIV screening tests on the market, it is not specific enough to be used as a one-step test and therefore requires confirmation. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2010-08-23T16:58:44Z 2010-11-04T14:19:50Z |
dc.date.available.fl_str_mv |
2010-11-04T14:19:50Z 2010-08-23T16:58:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OELEMANN, W. M. R. et al. Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study. Journal of Clinical Microbiology, Washington, v. 40, n. 3, p. 881–885, Mar. 2002. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/741 |
dc.identifier.issn.pt_BR.fl_str_mv |
1098-660X |
identifier_str_mv |
OELEMANN, W. M. R. et al. Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study. Journal of Clinical Microbiology, Washington, v. 40, n. 3, p. 881–885, Mar. 2002. 1098-660X |
url |
https://www.arca.fiocruz.br/handle/icict/741 |
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eng |
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eng |
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American Society for Microbiology |
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American Society for Microbiology |
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