A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.

Detalhes bibliográficos
Autor(a) principal: Assis, Bárbara Cristina Alves de
Data de Publicação: 2004
Outros Autores: Cunha, Liliane Monteiro, Baptista, Ana Paula, Andrade, Zilton de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3271
Resumo: Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
id CRUZ_95d5c0afe8cc9dbaa376368f4885d0f5
oai_identifier_str oai:www.arca.fiocruz.br:icict/3271
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Assis, Bárbara Cristina Alves deCunha, Liliane MonteiroBaptista, Ana PaulaAndrade, Zilton de Araújo2011-10-28T17:03:50Z2011-10-28T17:03:50Z2004ASSIS, B.C. et al. A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test. Memórias do Instituto Oswaldo Cruz, v. 99, n. 2, p. 173-177, mar. 2004.0074-0276https://www.arca.fiocruz.br/handle/icict/3271engA contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.A highly specific pattern of immunofluorescence was noted when sera from Capillaria hepatica-infected rats were tested against the homologous worms and eggs present either in paraffin or cryostat sections from mouse liver. The pattern was represented by a combined apple green fluorescence of the internal contents of worms and eggs, which persisted in serum-dilutions of 1:400 up to 1:1600. Unequivocal fluorescent pattern was observed from 15 days up to 3 months following inoculation of rats with embryonated C. hepatica eggs and such result was confirmed by the ELISA. After the 4th month of infection, the indirect immunofluorescence test turned negative, probably revealing the extinction of parasitism, however the ELISA was contradictory, disclosing high levels of antibodies in this period . The IIF was also negative when control normal rat sera and sera from rats administered by gavage with immature C. hepatica eggs (spurious infection), or for reactions made against Schistosoma mansoni eggs, although a weakly positive pattern occurred with Fasciola hepatica eggs. The indirect immunofluorescence test may be recommended for use with human sera to detect early C. hepatica infection in special clinical instances and in epidemiological surveys, since it is a simple, inexpensive, and reliable test, presenting excellent sensitivity and specificity. Although the diagnosis is positive only during early infection,Capillaria hepaticaImmunofluorescence testRat CapillariasisAnimaisAnticorpos Anti-HelmínticosCapillariaInfecções por EnoplidaELISAFemininoTécnica Indireta de Fluorescência para AnticorpoHepatopatias ParasitáriasMasculinoCamundongosContagem de Ovos de ParasitasRatosRatos WistarSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAssis BCA A Contribution to the diagnosis....pdfAssis BCA A Contribution to the diagnosis....pdfapplication/pdf116855https://www.arca.fiocruz.br/bitstream/icict/3271/1/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf1e7f45367b3b80614777ec4e05de6ef7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3271/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTAssis BCA A Contribution to the diagnosis....pdf.txtAssis BCA A Contribution to the diagnosis....pdf.txtExtracted texttext/plain21776https://www.arca.fiocruz.br/bitstream/icict/3271/5/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf.txtefa83bfcf5bcf962068e384c3f9fd5d3MD55THUMBNAILAssis BCA A Contribution to the diagnosis....pdf.jpgAssis BCA A Contribution to the diagnosis....pdf.jpgGenerated Thumbnailimage/jpeg2323https://www.arca.fiocruz.br/bitstream/icict/3271/4/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf.jpg4a7cfd35e6f9140755a41e6bb047b190MD54icict/32712023-03-15 14:32:58.016oai:www.arca.fiocruz.br:icict/3271Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKQnkgc2lnbmluZyBhbmQgc3VibWl0dGluZyB0aGlzIGxpY2Vuc2UsIHlvdSAodGhlIGF1dGhvcihzKSBvciBjb3B5cmlnaHQKb3duZXIpIGdyYW50cyB0byBEU3BhY2UgVW5pdmVyc2l0eSAoRFNVKSB0aGUgbm9uLWV4Y2x1c2l2ZSByaWdodCB0byByZXByb2R1Y2UsCnRyYW5zbGF0ZSAoYXMgZGVmaW5lZCBiZWxvdyksIGFuZC9vciBkaXN0cmlidXRlIHlvdXIgc3VibWlzc2lvbiAoaW5jbHVkaW5nCnRoZSBhYnN0cmFjdCkgd29ybGR3aWRlIGluIHByaW50IGFuZCBlbGVjdHJvbmljIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bSwKaW5jbHVkaW5nIGJ1dCBub3QgbGltaXRlZCB0byBhdWRpbyBvciB2aWRlby4KCllvdSBhZ3JlZSB0aGF0IERTVSBtYXksIHdpdGhvdXQgY2hhbmdpbmcgdGhlIGNvbnRlbnQsIHRyYW5zbGF0ZSB0aGUKc3VibWlzc2lvbiB0byBhbnkgbWVkaXVtIG9yIGZvcm1hdCBmb3IgdGhlIHB1cnBvc2Ugb2YgcHJlc2VydmF0aW9uLgoKWW91IGFsc28gYWdyZWUgdGhhdCBEU1UgbWF5IGtlZXAgbW9yZSB0aGFuIG9uZSBjb3B5IG9mIHRoaXMgc3VibWlzc2lvbiBmb3IKcHVycG9zZXMgb2Ygc2VjdXJpdHksIGJhY2stdXAgYW5kIHByZXNlcnZhdGlvbi4KCllvdSByZXByZXNlbnQgdGhhdCB0aGUgc3VibWlzc2lvbiBpcyB5b3VyIG9yaWdpbmFsIHdvcmssIGFuZCB0aGF0IHlvdSBoYXZlCnRoZSByaWdodCB0byBncmFudCB0aGUgcmlnaHRzIGNvbnRhaW5lZCBpbiB0aGlzIGxpY2Vuc2UuIFlvdSBhbHNvIHJlcHJlc2VudAp0aGF0IHlvdXIgc3VibWlzc2lvbiBkb2VzIG5vdCwgdG8gdGhlIGJlc3Qgb2YgeW91ciBrbm93bGVkZ2UsIGluZnJpbmdlIHVwb24KYW55b25lJ3MgY29weXJpZ2h0LgoKSWYgdGhlIHN1Ym1pc3Npb24gY29udGFpbnMgbWF0ZXJpYWwgZm9yIHdoaWNoIHlvdSBkbyBub3QgaG9sZCBjb3B5cmlnaHQsCnlvdSByZXByZXNlbnQgdGhhdCB5b3UgaGF2ZSBvYnRhaW5lZCB0aGUgdW5yZXN0cmljdGVkIHBlcm1pc3Npb24gb2YgdGhlCmNvcHlyaWdodCBvd25lciB0byBncmFudCBEU1UgdGhlIHJpZ2h0cyByZXF1aXJlZCBieSB0aGlzIGxpY2Vuc2UsIGFuZCB0aGF0CnN1Y2ggdGhpcmQtcGFydHkgb3duZWQgbWF0ZXJpYWwgaXMgY2xlYXJseSBpZGVudGlmaWVkIGFuZCBhY2tub3dsZWRnZWQKd2l0aGluIHRoZSB0ZXh0IG9yIGNvbnRlbnQgb2YgdGhlIHN1Ym1pc3Npb24uCgpJRiBUSEUgU1VCTUlTU0lPTiBJUyBCQVNFRCBVUE9OIFdPUksgVEhBVCBIQVMgQkVFTiBTUE9OU09SRUQgT1IgU1VQUE9SVEVECkJZIEFOIEFHRU5DWSBPUiBPUkdBTklaQVRJT04gT1RIRVIgVEhBTiBEU1UsIFlPVSBSRVBSRVNFTlQgVEhBVCBZT1UgSEFWRQpGVUxGSUxMRUQgQU5ZIFJJR0hUIE9GIFJFVklFVyBPUiBPVEhFUiBPQkxJR0FUSU9OUyBSRVFVSVJFRCBCWSBTVUNICkNPTlRSQUNUIE9SIEFHUkVFTUVOVC4KCkRTVSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgeW91ciBuYW1lKHMpIGFzIHRoZSBhdXRob3Iocykgb3Igb3duZXIocykgb2YgdGhlCnN1Ym1pc3Npb24sIGFuZCB3aWxsIG5vdCBtYWtlIGFueSBhbHRlcmF0aW9uLCBvdGhlciB0aGFuIGFzIGFsbG93ZWQgYnkgdGhpcwpsaWNlbnNlLCB0byB5b3VyIHN1Ym1pc3Npb24uCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:58Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
title A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
spellingShingle A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
Assis, Bárbara Cristina Alves de
Capillaria hepatica
Immunofluorescence test
Rat Capillariasis
Animais
Anticorpos Anti-Helmínticos
Capillaria
Infecções por Enoplida
ELISA
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Hepatopatias Parasitárias
Masculino
Camundongos
Contagem de Ovos de Parasitas
Ratos
Ratos Wistar
Sensibilidade e Especificidade
title_short A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
title_full A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
title_fullStr A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
title_full_unstemmed A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
title_sort A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test.
author Assis, Bárbara Cristina Alves de
author_facet Assis, Bárbara Cristina Alves de
Cunha, Liliane Monteiro
Baptista, Ana Paula
Andrade, Zilton de Araújo
author_role author
author2 Cunha, Liliane Monteiro
Baptista, Ana Paula
Andrade, Zilton de Araújo
author2_role author
author
author
dc.contributor.author.fl_str_mv Assis, Bárbara Cristina Alves de
Cunha, Liliane Monteiro
Baptista, Ana Paula
Andrade, Zilton de Araújo
dc.subject.en.pt_BR.fl_str_mv Capillaria hepatica
Immunofluorescence test
Rat Capillariasis
topic Capillaria hepatica
Immunofluorescence test
Rat Capillariasis
Animais
Anticorpos Anti-Helmínticos
Capillaria
Infecções por Enoplida
ELISA
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Hepatopatias Parasitárias
Masculino
Camundongos
Contagem de Ovos de Parasitas
Ratos
Ratos Wistar
Sensibilidade e Especificidade
dc.subject.decs.pt_BR.fl_str_mv Animais
Anticorpos Anti-Helmínticos
Capillaria
Infecções por Enoplida
ELISA
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Hepatopatias Parasitárias
Masculino
Camundongos
Contagem de Ovos de Parasitas
Ratos
Ratos Wistar
Sensibilidade e Especificidade
description Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2011-10-28T17:03:50Z
dc.date.available.fl_str_mv 2011-10-28T17:03:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ASSIS, B.C. et al. A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test. Memórias do Instituto Oswaldo Cruz, v. 99, n. 2, p. 173-177, mar. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3271
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv ASSIS, B.C. et al. A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test. Memórias do Instituto Oswaldo Cruz, v. 99, n. 2, p. 173-177, mar. 2004.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/3271
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/3271/1/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf
https://www.arca.fiocruz.br/bitstream/icict/3271/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/3271/5/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/3271/4/Assis%20BCA%20A%20Contribution%20to%20the%20diagnosis....pdf.jpg
bitstream.checksum.fl_str_mv 1e7f45367b3b80614777ec4e05de6ef7
e095249ac7cacefbfe39684dfe45e706
efa83bfcf5bcf962068e384c3f9fd5d3
4a7cfd35e6f9140755a41e6bb047b190
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1822792144775020544

Registros relacionados