From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes

Detalhes bibliográficos
Autor(a) principal: Dutra, Heverton Leandro Carneiro
Data de Publicação: 2015
Outros Autores: Santos, Lilha Maria Barbosa dos, Caragata, Eric Pearce, Silva, Jéssica Barreto Lopes, Villela, Daniel Antunes Maciel, Freitas, Rafael Maciel de, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12809
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.
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spelling Dutra, Heverton Leandro CarneiroSantos, Lilha Maria Barbosa dosCaragata, Eric PearceSilva, Jéssica Barreto LopesVillela, Daniel Antunes MacielFreitas, Rafael Maciel deMoreira, Luciano Andrade2016-02-25T17:29:23Z2016-02-25T17:29:23Z2015DUTRA, Heverton Leandro Carneiro et al. From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes. PLoS Negl Trop Dis., vol. 9, n. 4, p.e0003689, 2015.1935-2735https://www.arca.fiocruz.br/handle/icict/1280910.1371/journal.pntd.0003689engPublic Library of ScienceFrom Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.Laboratório de Transmissores de Hematozoários. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.BACKGROUND: The symbiotic bacterium Wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. Wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. Additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where this bacterium has been deployed have limited comparison and analysis of Wolbachia's invasive potential. METHODOLOGY/PRINCIPAL FINDINGS: We examined key phenotypic effects of the wMel Wolbachia strain in laboratory Aedes aegypti mosquitoes with a Brazilian genetic background to characterize its invasive potential. We show that the wMel strain causes strong cytoplasmic incompatibility, a high rate of maternal transmission and has no evident detrimental effect on host fecundity or fertility. Next, to understand the effects of different urban landscapes on the likelihood of mosquito survival, we performed mark-release-recapture experiments using Wolbachia-uninfected Brazilian mosquitoes in two areas of Rio de Janeiro where Wolbachia will be deployed in the future. We characterized the mosquito populations in relation to the socio-demographic conditions at these sites, and at three other future release areas. We then constructed mathematical models using both the laboratory and field data, and used these to describe the influence of urban environmental conditions on the likelihood that the Wolbachia infection frequency could reach 100% following mosquito release. We predict successful invasion at all five field sites, however the conditions by which this occurs vary greatly between sites, and are strongly influenced by the size of the local mosquito population. CONCLUSIONS/SIGNIFICANCE: Through analysis of laboratory, field and mathematical data, we show that the wMel strain of Wolbachia possesses the characteristics required to spread effectively in different urban socio-demographic environments in Rio de Janeiro, including those where mosquito releases from the Eliminate Dengue Program will take place.Aedes/microbiologyDengue/prevention & controlDengue/transmissionFertility/physiologyPest Control, Biological/methodsWolbachia/geneticsWolbachia/physiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12809/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFrom lab to field.pdfFrom lab to field.pdfapplication/pdf5075968https://www.arca.fiocruz.br/bitstream/icict/12809/2/From%20lab%20to%20field.pdf9eebb5062e030ecb060b60313066aa65MD52TEXTFrom 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dc.title.pt_BR.fl_str_mv From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
title From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
spellingShingle From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
Dutra, Heverton Leandro Carneiro
Aedes/microbiology
Dengue/prevention & control
Dengue/transmission
Fertility/physiology
Pest Control, Biological/methods
Wolbachia/genetics
Wolbachia/physiology
title_short From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
title_full From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
title_fullStr From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
title_full_unstemmed From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
title_sort From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes
author Dutra, Heverton Leandro Carneiro
author_facet Dutra, Heverton Leandro Carneiro
Santos, Lilha Maria Barbosa dos
Caragata, Eric Pearce
Silva, Jéssica Barreto Lopes
Villela, Daniel Antunes Maciel
Freitas, Rafael Maciel de
Moreira, Luciano Andrade
author_role author
author2 Santos, Lilha Maria Barbosa dos
Caragata, Eric Pearce
Silva, Jéssica Barreto Lopes
Villela, Daniel Antunes Maciel
Freitas, Rafael Maciel de
Moreira, Luciano Andrade
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dutra, Heverton Leandro Carneiro
Santos, Lilha Maria Barbosa dos
Caragata, Eric Pearce
Silva, Jéssica Barreto Lopes
Villela, Daniel Antunes Maciel
Freitas, Rafael Maciel de
Moreira, Luciano Andrade
dc.subject.en.pt_BR.fl_str_mv Aedes/microbiology
Dengue/prevention & control
Dengue/transmission
Fertility/physiology
Pest Control, Biological/methods
Wolbachia/genetics
Wolbachia/physiology
topic Aedes/microbiology
Dengue/prevention & control
Dengue/transmission
Fertility/physiology
Pest Control, Biological/methods
Wolbachia/genetics
Wolbachia/physiology
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-02-25T17:29:23Z
dc.date.available.fl_str_mv 2016-02-25T17:29:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUTRA, Heverton Leandro Carneiro et al. From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes. PLoS Negl Trop Dis., vol. 9, n. 4, p.e0003689, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12809
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003689
identifier_str_mv DUTRA, Heverton Leandro Carneiro et al. From Lab to Field: The Influence of Urban Landscapes on the Invasive Potential of Wolbachia in Brazilian Aedes aegypti Mosquitoes. PLoS Negl Trop Dis., vol. 9, n. 4, p.e0003689, 2015.
1935-2735
10.1371/journal.pntd.0003689
url https://www.arca.fiocruz.br/handle/icict/12809
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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