Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR

Detalhes bibliográficos
Autor(a) principal: Chagas Júnior, Adenizar Delgado das
Data de Publicação: 2012
Outros Autores: Silva, Caroline Luane Rabelo da, Soares, Luciane Marieta, Santos, Cleiton Silva, Silva, Carlos D. C. M, Athanazio, Daniel Abensur, Reis, Mitermayer Galvão dos, McBride, Flávia Weykamp da Cruz, McBride, Alan John Alexander
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5846
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
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spelling Chagas Júnior, Adenizar Delgado dasSilva, Caroline Luane Rabelo daSoares, Luciane MarietaSantos, Cleiton SilvaSilva, Carlos D. C. MAthanazio, Daniel AbensurReis, Mitermayer Galvão dosMcBride, Flávia Weykamp da CruzMcBride, Alan John Alexander2012-11-21T17:37:31Z2012-11-21T17:37:31Z2012CHAGAS JUNIOR, A.D. et al. Detection and Quantification of Leptospira interrogans in Hamster and and rat kidney samples: immunofluorescent imprints versus real-time PCR. PLoS One, v. 7, n. 2, p. e32712, 2012.1932-620310.1371/journal.pone.0032712https://www.arca.fiocruz.br/handle/icict/5846engDetection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFederal University of Bahia. Health Sciences Institute. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilA major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM) for the detection and quantification of leptospires in kidney samples from the rat and hamster experimental models of leptospirosis. Using a virulent strain of Leptospira interrogans serovar Copenhageni, a chronic infection was established in the rat model, which were euthanized 28 days post-infection, while the hamster model simulated an acute infection and the hamsters were euthanized eight days after inoculation. Leptospires in the kidney samples were detected using culture isolation, qPCR and the IM, and quantified using qPCR and the IM. In both the acute and chronic infection models, the correlation between quantification by qPCR and the IM was found to be positive and statistically significant (P<0.05). Therefore, this study demonstrates that the IM is a viable alternative for not only the detection but also the quantification of leptospires, particularly when the use of qPCR is not feasible.Rim/metabolismoLeptospira interrogans/metabolismoLeptospirose/microbiologiaMicroscopia de Fluorescência/métodosReação em Cadeia da Polimerase em Tempo Real/métodosAnimaisCricetinaeFemininoInfecçãoLeptospirose/patologiaMesocricetusRatosRatos WistarAnálise de Sequência de DNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALChagas Junior AD Detection and quantification....pdfChagas Junior AD Detection and quantification....pdfapplication/pdf178080https://www.arca.fiocruz.br/bitstream/icict/5846/1/Chagas%20Junior%20AD%20Detection%20and%20quantification....pdfb2561b9825462940327c01c8b52fc3cdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5846/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTChagas Junior AD Detection and quantification....pdf.txtChagas Junior AD Detection and quantification....pdf.txtExtracted texttext/plain24913https://www.arca.fiocruz.br/bitstream/icict/5846/5/Chagas%20Junior%20AD%20Detection%20and%20quantification....pdf.txt6bedbc94a4f9b7ea9e5ddb72cfeb2a56MD55THUMBNAILChagas Junior AD Detection and quantification....pdf.jpgChagas Junior AD Detection and quantification....pdf.jpgGenerated Thumbnailimage/jpeg2461https://www.arca.fiocruz.br/bitstream/icict/5846/4/Chagas%20Junior%20AD%20Detection%20and%20quantification....pdf.jpga386aeea0581c0a61051f12b4ccb01a9MD54icict/58462023-03-15 14:33:01.267oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
title Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
spellingShingle Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
Chagas Júnior, Adenizar Delgado das
Rim/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/microbiologia
Microscopia de Fluorescência/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Animais
Cricetinae
Feminino
Infecção
Leptospirose/patologia
Mesocricetus
Ratos
Ratos Wistar
Análise de Sequência de DNA
title_short Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
title_full Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
title_fullStr Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
title_full_unstemmed Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
title_sort Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR
author Chagas Júnior, Adenizar Delgado das
author_facet Chagas Júnior, Adenizar Delgado das
Silva, Caroline Luane Rabelo da
Soares, Luciane Marieta
Santos, Cleiton Silva
Silva, Carlos D. C. M
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia Weykamp da Cruz
McBride, Alan John Alexander
author_role author
author2 Silva, Caroline Luane Rabelo da
Soares, Luciane Marieta
Santos, Cleiton Silva
Silva, Carlos D. C. M
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia Weykamp da Cruz
McBride, Alan John Alexander
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chagas Júnior, Adenizar Delgado das
Silva, Caroline Luane Rabelo da
Soares, Luciane Marieta
Santos, Cleiton Silva
Silva, Carlos D. C. M
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia Weykamp da Cruz
McBride, Alan John Alexander
dc.subject.decs.pt_BR.fl_str_mv Rim/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/microbiologia
Microscopia de Fluorescência/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Animais
Cricetinae
Feminino
Infecção
Leptospirose/patologia
Mesocricetus
Ratos
Ratos Wistar
Análise de Sequência de DNA
topic Rim/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/microbiologia
Microscopia de Fluorescência/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Animais
Cricetinae
Feminino
Infecção
Leptospirose/patologia
Mesocricetus
Ratos
Ratos Wistar
Análise de Sequência de DNA
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
publishDate 2012
dc.date.accessioned.fl_str_mv 2012-11-21T17:37:31Z
dc.date.available.fl_str_mv 2012-11-21T17:37:31Z
dc.date.issued.fl_str_mv 2012
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAGAS JUNIOR, A.D. et al. Detection and Quantification of Leptospira interrogans in Hamster and and rat kidney samples: immunofluorescent imprints versus real-time PCR. PLoS One, v. 7, n. 2, p. e32712, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5846
dc.identifier.issn.none.fl_str_mv 1932-6203
10.1371/journal.pone.0032712
identifier_str_mv CHAGAS JUNIOR, A.D. et al. Detection and Quantification of Leptospira interrogans in Hamster and and rat kidney samples: immunofluorescent imprints versus real-time PCR. PLoS One, v. 7, n. 2, p. e32712, 2012.
1932-6203
10.1371/journal.pone.0032712
url https://www.arca.fiocruz.br/handle/icict/5846
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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