Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15185 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Tavares, NatáliaAfonso, Lilian MariaSuarez, MarthaAmpuero, MarianaPrates, Deboraci BritoSantos, Théo AraújoBarral Netto, ManoelDosReis, George AlexandreBorges, Valéria de MatosBrodskyn, Claudia Ida2016-08-02T18:42:20Z2016-08-02T18:42:20Z2016TAVARES, N. et al. Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites. The Journal of Immunology, v. 196, p. 1865-1873, 2016.0022-1767https://www.arca.fiocruz.br/handle/icict/1518510.4049/jimmunol.1502224engAmerican Association of Immunologists, Inc.Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilNeutrophils mediate early responses against pathogens, and they become activated during endothelial transmigration toward the inflammatory site. In the current study, human neutrophils were activated in vitro with immobilized extracellular matrix proteins, such as fibronectin (FN), collagen, and laminin. Neutrophil activation by FN, but not other extracellular matrix proteins, induces the release of the granules' contents, measured as matrix metalloproteinase 9 and neutrophil elastase activity in culture supernatant, as well as reactive oxygen species production. Upon contact with Leishmania amazonensis-infected macrophages, these FN-activated neutrophils reduce the parasite burden through a mechanism independent of cell contact. The release of granule proteases, such as myeloperoxidase, neutrophil elastase, and matrix metalloproteinase 9, activates macrophages through TLRs, leading to the production of inflammatory mediators, TNF-α and leukotriene B4 (LTB4), which are involved in parasite killing by infected macrophages. The pharmacological inhibition of degranulation reverted this effect, abolishing LTB4 and TNF production. Together, these results suggest that FN-driven degranulation of neutrophils induces the production of LTB4 and TNF by infected macrophages, leading to the control of Leishmania infection.LeishmaniaLeishmania mexicanaLeishmaniasis cutaneousCell degranulationFibronectinsHumansMacrophagesMicroscopy electronNeutrophilsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15185/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTavares Degranulation... 2016.pdfTavares Degranulation... 2016.pdfapplication/pdf1492596https://www.arca.fiocruz.br/bitstream/icict/15185/2/Tavares%20Degranulation...%202016.pdfa4024e14fec7c89c81c19a5f752e5411MD52TEXTTavares Degranulation... 2016.pdf.txtTavares Degranulation... 2016.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| title |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| spellingShingle |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites Tavares, Natália Leishmania Leishmania mexicana Leishmaniasis cutaneous Cell degranulation Fibronectins Humans Macrophages Microscopy electron Neutrophils |
| title_short |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| title_full |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| title_fullStr |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| title_full_unstemmed |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| title_sort |
Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites |
| author |
Tavares, Natália |
| author_facet |
Tavares, Natália Afonso, Lilian Maria Suarez, Martha Ampuero, Mariana Prates, Deboraci Brito Santos, Théo Araújo Barral Netto, Manoel DosReis, George Alexandre Borges, Valéria de Matos Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Afonso, Lilian Maria Suarez, Martha Ampuero, Mariana Prates, Deboraci Brito Santos, Théo Araújo Barral Netto, Manoel DosReis, George Alexandre Borges, Valéria de Matos Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Tavares, Natália Afonso, Lilian Maria Suarez, Martha Ampuero, Mariana Prates, Deboraci Brito Santos, Théo Araújo Barral Netto, Manoel DosReis, George Alexandre Borges, Valéria de Matos Brodskyn, Claudia Ida |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Leishmania mexicana Leishmaniasis cutaneous Cell degranulation Fibronectins Humans Macrophages Microscopy electron Neutrophils |
| topic |
Leishmania Leishmania mexicana Leishmaniasis cutaneous Cell degranulation Fibronectins Humans Macrophages Microscopy electron Neutrophils |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
| publishDate |
2016 |
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2016-08-02T18:42:20Z |
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2016-08-02T18:42:20Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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TAVARES, N. et al. Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites. The Journal of Immunology, v. 196, p. 1865-1873, 2016. |
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https://www.arca.fiocruz.br/handle/icict/15185 |
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0022-1767 |
| dc.identifier.doi.none.fl_str_mv |
10.4049/jimmunol.1502224 |
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TAVARES, N. et al. Degranulating Neutrophils Promote Leukotriene B4 Production by Infected Macrophages To Kill Leishmania amazonensis Parasites. The Journal of Immunology, v. 196, p. 1865-1873, 2016. 0022-1767 10.4049/jimmunol.1502224 |
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American Association of Immunologists, Inc. |
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American Association of Immunologists, Inc. |
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