Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.

Detalhes bibliográficos
Autor(a) principal: Pereira, Felicidade Mota
Data de Publicação: 2014
Outros Autores: Zarife, Maria Alice Sant’Anna, Reis, Eliana Almeida Gomes, Reis, Mitermayer Galvão dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7795
Resumo: Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
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spelling Pereira, Felicidade MotaZarife, Maria Alice Sant’AnnaReis, Eliana Almeida GomesReis, Mitermayer Galvão dos2014-06-04T19:18:47Z2014-06-04T19:18:47Z2014PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014.1678-9849https://www.arca.fiocruz.br/handle/icict/779510.1590/0037-8682-0222-2013engSociedade Brasileira de Medicina TropicalIndeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilLaboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Faculdade de Medicina da Bahia. Universidade Federal da Bahia. Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, BrasilINTRODUCTION: Hepatitis C virus (HCV) infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA) and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies. METHODS: A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA) at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA) were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany), the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA), the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA) and line probe assay (LiPA - Siemens, Tarrytown, NY, USA) genotyping for HCV diagnosis. RESULTS: Of these new samples, 38.2% (39/102) were positive, 57.8% (59/102) were negative and 3.9% (4/102) were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102) of the samples. RIBA results were positive in 58.1% (25/43), negative in 9.3% (4/43) and indeterminate in 32.6% (14/43) of the samples. The prevailing genotypes were 1 (78.3%, 18/23), 3 (17.4%, 4/23) and 2 (4.3%, 1/23). All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL). Of these samples, 71.4% (10/14) were reevaluated six months later. Eighty percent (8/10) of these samples remained indeterminate by RIBA, and 20% (2/10) were negative. CONCLUSIONS: In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA.Blood donorsHepatitis C virusAnti-HCVRIBA.HCV-RNADoadores de SangueHepacivirus/genéticaAnticorpos Anti-Hepatite C/sangueHepatite C/diagnósticoRNA Viral/sangueEnsaio de Imunoadsorção EnzimáticaAdultoFemininoGenótipoHepacivirus/imunologiaHumanosImmunoblottingMasculinoReação em Cadeia da PolimeraseProteínas Recombinantes/uso diagnósticoCarga Viralinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPereira FM Indeterminate RIBA....pdfPereira FM Indeterminate RIBA....pdfapplication/pdf372129https://www.arca.fiocruz.br/bitstream/icict/7795/1/Pereira%20FM%20Indeterminate%20RIBA....pdf5a18f73bcfe14c72f1a54fcf942a487aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7795/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPereira FM Indeterminate RIBA....pdf.txtPereira FM Indeterminate RIBA....pdf.txtExtracted texttext/plain26059https://www.arca.fiocruz.br/bitstream/icict/7795/5/Pereira%20FM%20Indeterminate%20RIBA....pdf.txtc61642725fd2ff090866eac9e3f3311fMD55THUMBNAILPereira FM Indeterminate RIBA....pdf.jpgPereira FM Indeterminate RIBA....pdf.jpgGenerated Thumbnailimage/jpeg2469https://www.arca.fiocruz.br/bitstream/icict/7795/4/Pereira%20FM%20Indeterminate%20RIBA....pdf.jpg49b6940e58a0409ce2573fefb85a51ddMD54icict/77952023-03-15 14:33:17.284oai:www.arca.fiocruz.br:icict/7795TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
title Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
spellingShingle Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
Pereira, Felicidade Mota
Blood donors
Hepatitis C virus
Anti-HCV
RIBA.
HCV-RNA
Doadores de Sangue
Hepacivirus/genética
Anticorpos Anti-Hepatite C/sangue
Hepatite C/diagnóstico
RNA Viral/sangue
Ensaio de Imunoadsorção Enzimática
Adulto
Feminino
Genótipo
Hepacivirus/imunologia
Humanos
Immunoblotting
Masculino
Reação em Cadeia da Polimerase
Proteínas Recombinantes/uso diagnóstico
Carga Viral
title_short Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
title_full Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
title_fullStr Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
title_full_unstemmed Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
title_sort Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
author Pereira, Felicidade Mota
author_facet Pereira, Felicidade Mota
Zarife, Maria Alice Sant’Anna
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
author_role author
author2 Zarife, Maria Alice Sant’Anna
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Pereira, Felicidade Mota
Zarife, Maria Alice Sant’Anna
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
dc.subject.en.pt_BR.fl_str_mv Blood donors
Hepatitis C virus
Anti-HCV
RIBA.
HCV-RNA
topic Blood donors
Hepatitis C virus
Anti-HCV
RIBA.
HCV-RNA
Doadores de Sangue
Hepacivirus/genética
Anticorpos Anti-Hepatite C/sangue
Hepatite C/diagnóstico
RNA Viral/sangue
Ensaio de Imunoadsorção Enzimática
Adulto
Feminino
Genótipo
Hepacivirus/imunologia
Humanos
Immunoblotting
Masculino
Reação em Cadeia da Polimerase
Proteínas Recombinantes/uso diagnóstico
Carga Viral
dc.subject.decs.pt_BR.fl_str_mv Doadores de Sangue
Hepacivirus/genética
Anticorpos Anti-Hepatite C/sangue
Hepatite C/diagnóstico
RNA Viral/sangue
Ensaio de Imunoadsorção Enzimática
Adulto
Feminino
Genótipo
Hepacivirus/imunologia
Humanos
Immunoblotting
Masculino
Reação em Cadeia da Polimerase
Proteínas Recombinantes/uso diagnóstico
Carga Viral
description Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-06-04T19:18:47Z
dc.date.available.fl_str_mv 2014-06-04T19:18:47Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7795
dc.identifier.issn.none.fl_str_mv 1678-9849
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0222-2013
identifier_str_mv PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014.
1678-9849
10.1590/0037-8682-0222-2013
url https://www.arca.fiocruz.br/handle/icict/7795
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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