Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7795 |
Resumo: | Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil |
id |
CRUZ_98924d9f437d15edd5bc29a5dfbd2730 |
---|---|
oai_identifier_str |
oai:www.arca.fiocruz.br:icict/7795 |
network_acronym_str |
CRUZ |
network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
repository_id_str |
2135 |
spelling |
Pereira, Felicidade MotaZarife, Maria Alice Sant’AnnaReis, Eliana Almeida GomesReis, Mitermayer Galvão dos2014-06-04T19:18:47Z2014-06-04T19:18:47Z2014PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014.1678-9849https://www.arca.fiocruz.br/handle/icict/779510.1590/0037-8682-0222-2013engSociedade Brasileira de Medicina TropicalIndeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilLaboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Faculdade de Medicina da Bahia. Universidade Federal da Bahia. Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, BrasilINTRODUCTION: Hepatitis C virus (HCV) infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA) and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies. METHODS: A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA) at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA) were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany), the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA), the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA) and line probe assay (LiPA - Siemens, Tarrytown, NY, USA) genotyping for HCV diagnosis. RESULTS: Of these new samples, 38.2% (39/102) were positive, 57.8% (59/102) were negative and 3.9% (4/102) were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102) of the samples. RIBA results were positive in 58.1% (25/43), negative in 9.3% (4/43) and indeterminate in 32.6% (14/43) of the samples. The prevailing genotypes were 1 (78.3%, 18/23), 3 (17.4%, 4/23) and 2 (4.3%, 1/23). All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL). Of these samples, 71.4% (10/14) were reevaluated six months later. Eighty percent (8/10) of these samples remained indeterminate by RIBA, and 20% (2/10) were negative. CONCLUSIONS: In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA.Blood donorsHepatitis C virusAnti-HCVRIBA.HCV-RNADoadores de SangueHepacivirus/genéticaAnticorpos Anti-Hepatite C/sangueHepatite C/diagnósticoRNA Viral/sangueEnsaio de Imunoadsorção EnzimáticaAdultoFemininoGenótipoHepacivirus/imunologiaHumanosImmunoblottingMasculinoReação em Cadeia da PolimeraseProteínas Recombinantes/uso diagnósticoCarga Viralinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPereira FM Indeterminate RIBA....pdfPereira FM Indeterminate RIBA....pdfapplication/pdf372129https://www.arca.fiocruz.br/bitstream/icict/7795/1/Pereira%20FM%20Indeterminate%20RIBA....pdf5a18f73bcfe14c72f1a54fcf942a487aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7795/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPereira FM Indeterminate RIBA....pdf.txtPereira FM Indeterminate RIBA....pdf.txtExtracted texttext/plain26059https://www.arca.fiocruz.br/bitstream/icict/7795/5/Pereira%20FM%20Indeterminate%20RIBA....pdf.txtc61642725fd2ff090866eac9e3f3311fMD55THUMBNAILPereira FM Indeterminate RIBA....pdf.jpgPereira FM Indeterminate RIBA....pdf.jpgGenerated Thumbnailimage/jpeg2469https://www.arca.fiocruz.br/bitstream/icict/7795/4/Pereira%20FM%20Indeterminate%20RIBA....pdf.jpg49b6940e58a0409ce2573fefb85a51ddMD54icict/77952023-03-15 14:33:17.284oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
title |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
spellingShingle |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Pereira, Felicidade Mota Blood donors Hepatitis C virus Anti-HCV RIBA. HCV-RNA Doadores de Sangue Hepacivirus/genética Anticorpos Anti-Hepatite C/sangue Hepatite C/diagnóstico RNA Viral/sangue Ensaio de Imunoadsorção Enzimática Adulto Feminino Genótipo Hepacivirus/imunologia Humanos Immunoblotting Masculino Reação em Cadeia da Polimerase Proteínas Recombinantes/uso diagnóstico Carga Viral |
title_short |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
title_full |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
title_fullStr |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
title_full_unstemmed |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
title_sort |
Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. |
author |
Pereira, Felicidade Mota |
author_facet |
Pereira, Felicidade Mota Zarife, Maria Alice Sant’Anna Reis, Eliana Almeida Gomes Reis, Mitermayer Galvão dos |
author_role |
author |
author2 |
Zarife, Maria Alice Sant’Anna Reis, Eliana Almeida Gomes Reis, Mitermayer Galvão dos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pereira, Felicidade Mota Zarife, Maria Alice Sant’Anna Reis, Eliana Almeida Gomes Reis, Mitermayer Galvão dos |
dc.subject.en.pt_BR.fl_str_mv |
Blood donors Hepatitis C virus Anti-HCV RIBA. HCV-RNA |
topic |
Blood donors Hepatitis C virus Anti-HCV RIBA. HCV-RNA Doadores de Sangue Hepacivirus/genética Anticorpos Anti-Hepatite C/sangue Hepatite C/diagnóstico RNA Viral/sangue Ensaio de Imunoadsorção Enzimática Adulto Feminino Genótipo Hepacivirus/imunologia Humanos Immunoblotting Masculino Reação em Cadeia da Polimerase Proteínas Recombinantes/uso diagnóstico Carga Viral |
dc.subject.decs.pt_BR.fl_str_mv |
Doadores de Sangue Hepacivirus/genética Anticorpos Anti-Hepatite C/sangue Hepatite C/diagnóstico RNA Viral/sangue Ensaio de Imunoadsorção Enzimática Adulto Feminino Genótipo Hepacivirus/imunologia Humanos Immunoblotting Masculino Reação em Cadeia da Polimerase Proteínas Recombinantes/uso diagnóstico Carga Viral |
description |
Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-06-04T19:18:47Z |
dc.date.available.fl_str_mv |
2014-06-04T19:18:47Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7795 |
dc.identifier.issn.none.fl_str_mv |
1678-9849 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0037-8682-0222-2013 |
identifier_str_mv |
PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014. 1678-9849 10.1590/0037-8682-0222-2013 |
url |
https://www.arca.fiocruz.br/handle/icict/7795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/7795/1/Pereira%20FM%20Indeterminate%20RIBA....pdf https://www.arca.fiocruz.br/bitstream/icict/7795/2/license.txt https://www.arca.fiocruz.br/bitstream/icict/7795/5/Pereira%20FM%20Indeterminate%20RIBA....pdf.txt https://www.arca.fiocruz.br/bitstream/icict/7795/4/Pereira%20FM%20Indeterminate%20RIBA....pdf.jpg |
bitstream.checksum.fl_str_mv |
5a18f73bcfe14c72f1a54fcf942a487a 7d48279ffeed55da8dfe2f8e81f3b81f c61642725fd2ff090866eac9e3f3311f 49b6940e58a0409ce2573fefb85a51dd |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
_version_ |
1813009180076802048 |