Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Flávia Guimarães
Data de Publicação: 2006
Outros Autores: Oliveira, Sabrina Barbosa, Rocha, Bruno Coelho, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6578
Resumo: TDR/WHO (grant A20741) Fapemig research scholarship
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spelling Rodrigues, Flávia GuimarãesOliveira, Sabrina BarbosaRocha, Bruno CoelhoMoreira, Luciano Andrade2013-06-11T16:23:05Z2013-06-11T16:23:05Z2006RODRIGUES, Flávia Guimarães et al. Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element. Mem. Inst. Oswaldo Cruz. 2006, vol.101, n.7 pp. 755-7570074-0276https://www.arca.fiocruz.br/handle/icict/657810.1590/S0074-02762006000700008TDR/WHO (grant A20741) Fapemig research scholarshipFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilThe technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.engGermline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable elementinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletransgenic mosquitoespiggyBacmalariainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL104.pdf104.pdfapplication/pdf3056009https://www.arca.fiocruz.br/bitstream/icict/6578/1/104.pdfd944861d9f7d5a0e96023b3e179e5328MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6578/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT104.pdf.txt104.pdf.txtExtracted texttext/plain13538https://www.arca.fiocruz.br/bitstream/icict/6578/5/104.pdf.txt121d585650daba39d337e311bbba9409MD55THUMBNAIL104.pdf.jpg104.pdf.jpgGenerated Thumbnailimage/jpeg2391https://www.arca.fiocruz.br/bitstream/icict/6578/4/104.pdf.jpge9d0fc38cc6d1205102233ebc9fd4062MD54icict/65782018-05-18 09:22:11.894oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-18T12:22:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
spellingShingle Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
Rodrigues, Flávia Guimarães
transgenic mosquitoes
piggyBac
malaria
title_short Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_full Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_fullStr Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_full_unstemmed Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_sort Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
author Rodrigues, Flávia Guimarães
author_facet Rodrigues, Flávia Guimarães
Oliveira, Sabrina Barbosa
Rocha, Bruno Coelho
Moreira, Luciano Andrade
author_role author
author2 Oliveira, Sabrina Barbosa
Rocha, Bruno Coelho
Moreira, Luciano Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Flávia Guimarães
Oliveira, Sabrina Barbosa
Rocha, Bruno Coelho
Moreira, Luciano Andrade
dc.subject.en.pt_BR.fl_str_mv transgenic mosquitoes
piggyBac
malaria
topic transgenic mosquitoes
piggyBac
malaria
description TDR/WHO (grant A20741) Fapemig research scholarship
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-06-11T16:23:05Z
dc.date.available.fl_str_mv 2013-06-11T16:23:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUES, Flávia Guimarães et al. Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element. Mem. Inst. Oswaldo Cruz. 2006, vol.101, n.7 pp. 755-757
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6578
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000700008
identifier_str_mv RODRIGUES, Flávia Guimarães et al. Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element. Mem. Inst. Oswaldo Cruz. 2006, vol.101, n.7 pp. 755-757
0074-0276
10.1590/S0074-02762006000700008
url https://www.arca.fiocruz.br/handle/icict/6578
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instacron_str FIOCRUZ
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