Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Flávia Guimarães
Data de Publicação: 2006
Outros Autores: Oliveira,Sabrina Barbosa, Rocha,Bruno Coelho, Moreira,Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700008
Resumo: The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
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spelling Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable elementtransgenic mosquitoespiggyBacmalariaThe technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.Instituto Oswaldo Cruz, Ministério da Saúde2006-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700008Memórias do Instituto Oswaldo Cruz v.101 n.7 2006reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762006000700008info:eu-repo/semantics/openAccessRodrigues,Flávia GuimarãesOliveira,Sabrina BarbosaRocha,Bruno CoelhoMoreira,Luciano Andradeeng2020-04-25T17:49:40Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:14:00.457Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
spellingShingle Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
Rodrigues,Flávia Guimarães
transgenic mosquitoes
piggyBac
malaria
title_short Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_full Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_fullStr Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_full_unstemmed Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
title_sort Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
author Rodrigues,Flávia Guimarães
author_facet Rodrigues,Flávia Guimarães
Oliveira,Sabrina Barbosa
Rocha,Bruno Coelho
Moreira,Luciano Andrade
author_role author
author2 Oliveira,Sabrina Barbosa
Rocha,Bruno Coelho
Moreira,Luciano Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Rodrigues,Flávia Guimarães
Oliveira,Sabrina Barbosa
Rocha,Bruno Coelho
Moreira,Luciano Andrade
dc.subject.por.fl_str_mv transgenic mosquitoes
piggyBac
malaria
topic transgenic mosquitoes
piggyBac
malaria
dc.description.none.fl_txt_mv The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
description The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
publishDate 2006
dc.date.none.fl_str_mv 2006-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700008
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762006000700008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.101 n.7 2006
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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