A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers

Detalhes bibliográficos
Autor(a) principal: Deprá,Maríndia
Data de Publicação: 2004
Outros Autores: Sepel,Lenira Maria Nunes, Loreto,Élgion Lucio da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
Resumo: We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
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spelling A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markerstransgenic DrosophilapiggyBacGFPtransposable elementsWe describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012Genetics and Molecular Biology v.27 n.1 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000100012info:eu-repo/semantics/openAccessDeprá,MaríndiaSepel,Lenira Maria NunesLoreto,Élgion Lucio da Silvaeng2004-04-13T00:00:00Zoai:scielo:S1415-47572004000100012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-04-13T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
spellingShingle A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Deprá,Maríndia
transgenic Drosophila
piggyBac
GFP
transposable elements
title_short A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_full A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_fullStr A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_full_unstemmed A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_sort A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
author Deprá,Maríndia
author_facet Deprá,Maríndia
Sepel,Lenira Maria Nunes
Loreto,Élgion Lucio da Silva
author_role author
author2 Sepel,Lenira Maria Nunes
Loreto,Élgion Lucio da Silva
author2_role author
author
dc.contributor.author.fl_str_mv Deprá,Maríndia
Sepel,Lenira Maria Nunes
Loreto,Élgion Lucio da Silva
dc.subject.por.fl_str_mv transgenic Drosophila
piggyBac
GFP
transposable elements
topic transgenic Drosophila
piggyBac
GFP
transposable elements
description We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000100012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.1 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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