M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity

Detalhes bibliográficos
Autor(a) principal: Schwarz, Marcos Gustavo Araujo
Data de Publicação: 2021
Outros Autores: Luzes, Bianca Gallart Cinelli, Correa, Paloma Rezende, Gonçalves, Antônio José da Silva, Machado, Lucas de Almeida, Guimarães, Ana Carolina Ramos, Lima, Leila Mendonça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46653
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Schwarz, Marcos Gustavo AraujoLuzes, Bianca Gallart CinelliCorrea, Paloma RezendeGonçalves, Antônio José da SilvaMachado, Lucas de AlmeidaGuimarães, Ana Carolina RamosLima, Leila Mendonça2021-04-12T18:46:49Z2021-04-12T18:46:49Z2021SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021.2235-2988https://www.arca.fiocruz.br/handle/icict/4665310.3389/fcimb.2021.658888engFrontiers MediaPerda do Terminal NM. bovis BCG MoreauDodecina menos estávelLigação de flavina inferiorM. bovis BCG MoreauN-Terminal LossDodecinLower FlavinBinding CapacityM. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Tuberculosis still remains a concerning health problem worldwide. Its etiologic agent, Mycobacterium tuberculosis, continues to be the focus of research to unravel new prophylactic and therapeutic strategies against this disease. The only vaccine in use against tuberculosis is based on the in vitro attenuated strain, M. bovis BCG. Dodecin is a dodecameric complex important for flavin homeostasis in Archea and Eubacteria, and the M. tuberculosis protein is described as thermo- and halostable. M. bovis BCG Moreau, the Brazilian vaccine strain, has a single nucleotide polymorphism in the dodecin start codon, leading to a predicted loss of seven amino acids at the protein N-terminal end. In this work we aimed to characterize the effect of this mutation in the BCG Moreau protein features. Our recombinant protein assays show that the predicted BCG homolog is less thermostable than M.tb’s but maintains its dodecamerization ability, although with a lower riboflavin-binding capacity. These data are corroborated by structural analysis after comparative modeling, showing that the predicted BCG dodecin complex has a lower interaction energy among its monomers and also a distinct electrostatic surface near the flavin binding pocket. However, western blotting assays with the native proteins were unable to detect significant differences between the BCG Moreau and M.tb orthologs, indicating that other factors may be modulating protein structure/function in the bacterial context.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46653/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosSchwarz_LeilaMLima_etalIOC_2021.pdfMarcosSchwarz_LeilaMLima_etalIOC_2021.pdfapplication/pdf2002622https://www.arca.fiocruz.br/bitstream/icict/46653/2/MarcosSchwarz_LeilaMLima_etalIOC_2021.pdf4830706ef183aa1ff60a0ec7db5fcd74MD52TEXTMarcosSchwarz_LeilaMLima_etalIOC_2021.pdf.txtMarcosSchwarz_LeilaMLima_etalIOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
title M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
spellingShingle M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
Schwarz, Marcos Gustavo Araujo
Perda do Terminal N
M. bovis BCG Moreau
Dodecina menos estável
Ligação de flavina inferior
M. bovis BCG Moreau
N-Terminal Loss
Dodecin
Lower Flavin
Binding Capacity
title_short M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
title_full M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
title_fullStr M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
title_full_unstemmed M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
title_sort M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
author Schwarz, Marcos Gustavo Araujo
author_facet Schwarz, Marcos Gustavo Araujo
Luzes, Bianca Gallart Cinelli
Correa, Paloma Rezende
Gonçalves, Antônio José da Silva
Machado, Lucas de Almeida
Guimarães, Ana Carolina Ramos
Lima, Leila Mendonça
author_role author
author2 Luzes, Bianca Gallart Cinelli
Correa, Paloma Rezende
Gonçalves, Antônio José da Silva
Machado, Lucas de Almeida
Guimarães, Ana Carolina Ramos
Lima, Leila Mendonça
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schwarz, Marcos Gustavo Araujo
Luzes, Bianca Gallart Cinelli
Correa, Paloma Rezende
Gonçalves, Antônio José da Silva
Machado, Lucas de Almeida
Guimarães, Ana Carolina Ramos
Lima, Leila Mendonça
dc.subject.other.pt_BR.fl_str_mv Perda do Terminal N
M. bovis BCG Moreau
Dodecina menos estável
Ligação de flavina inferior
topic Perda do Terminal N
M. bovis BCG Moreau
Dodecina menos estável
Ligação de flavina inferior
M. bovis BCG Moreau
N-Terminal Loss
Dodecin
Lower Flavin
Binding Capacity
dc.subject.en.pt_BR.fl_str_mv M. bovis BCG Moreau
N-Terminal Loss
Dodecin
Lower Flavin
Binding Capacity
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-04-12T18:46:49Z
dc.date.available.fl_str_mv 2021-04-12T18:46:49Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46653
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2021.658888
identifier_str_mv SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021.
2235-2988
10.3389/fcimb.2021.658888
url https://www.arca.fiocruz.br/handle/icict/46653
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/46653/2/MarcosSchwarz_LeilaMLima_etalIOC_2021.pdf
https://www.arca.fiocruz.br/bitstream/icict/46653/3/MarcosSchwarz_LeilaMLima_etalIOC_2021.pdf.txt
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