M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46653 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. |
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Schwarz, Marcos Gustavo AraujoLuzes, Bianca Gallart CinelliCorrea, Paloma RezendeGonçalves, Antônio José da SilvaMachado, Lucas de AlmeidaGuimarães, Ana Carolina RamosLima, Leila Mendonça2021-04-12T18:46:49Z2021-04-12T18:46:49Z2021SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021.2235-2988https://www.arca.fiocruz.br/handle/icict/4665310.3389/fcimb.2021.658888engFrontiers MediaPerda do Terminal NM. bovis BCG MoreauDodecina menos estávelLigação de flavina inferiorM. bovis BCG MoreauN-Terminal LossDodecinLower FlavinBinding CapacityM. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Tuberculosis still remains a concerning health problem worldwide. Its etiologic agent, Mycobacterium tuberculosis, continues to be the focus of research to unravel new prophylactic and therapeutic strategies against this disease. The only vaccine in use against tuberculosis is based on the in vitro attenuated strain, M. bovis BCG. Dodecin is a dodecameric complex important for flavin homeostasis in Archea and Eubacteria, and the M. tuberculosis protein is described as thermo- and halostable. M. bovis BCG Moreau, the Brazilian vaccine strain, has a single nucleotide polymorphism in the dodecin start codon, leading to a predicted loss of seven amino acids at the protein N-terminal end. In this work we aimed to characterize the effect of this mutation in the BCG Moreau protein features. Our recombinant protein assays show that the predicted BCG homolog is less thermostable than M.tb’s but maintains its dodecamerization ability, although with a lower riboflavin-binding capacity. These data are corroborated by structural analysis after comparative modeling, showing that the predicted BCG dodecin complex has a lower interaction energy among its monomers and also a distinct electrostatic surface near the flavin binding pocket. However, western blotting assays with the native proteins were unable to detect significant differences between the BCG Moreau and M.tb orthologs, indicating that other factors may be modulating protein structure/function in the bacterial context.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46653/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosSchwarz_LeilaMLima_etalIOC_2021.pdfMarcosSchwarz_LeilaMLima_etalIOC_2021.pdfapplication/pdf2002622https://www.arca.fiocruz.br/bitstream/icict/46653/2/MarcosSchwarz_LeilaMLima_etalIOC_2021.pdf4830706ef183aa1ff60a0ec7db5fcd74MD52TEXTMarcosSchwarz_LeilaMLima_etalIOC_2021.pdf.txtMarcosSchwarz_LeilaMLima_etalIOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
title |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
spellingShingle |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity Schwarz, Marcos Gustavo Araujo Perda do Terminal N M. bovis BCG Moreau Dodecina menos estável Ligação de flavina inferior M. bovis BCG Moreau N-Terminal Loss Dodecin Lower Flavin Binding Capacity |
title_short |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
title_full |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
title_fullStr |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
title_full_unstemmed |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
title_sort |
M. bovis BCG Moreau N-Terminal Loss Leads to a Less Stable Dodecin With Lower Flavin Binding Capacity |
author |
Schwarz, Marcos Gustavo Araujo |
author_facet |
Schwarz, Marcos Gustavo Araujo Luzes, Bianca Gallart Cinelli Correa, Paloma Rezende Gonçalves, Antônio José da Silva Machado, Lucas de Almeida Guimarães, Ana Carolina Ramos Lima, Leila Mendonça |
author_role |
author |
author2 |
Luzes, Bianca Gallart Cinelli Correa, Paloma Rezende Gonçalves, Antônio José da Silva Machado, Lucas de Almeida Guimarães, Ana Carolina Ramos Lima, Leila Mendonça |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Schwarz, Marcos Gustavo Araujo Luzes, Bianca Gallart Cinelli Correa, Paloma Rezende Gonçalves, Antônio José da Silva Machado, Lucas de Almeida Guimarães, Ana Carolina Ramos Lima, Leila Mendonça |
dc.subject.other.pt_BR.fl_str_mv |
Perda do Terminal N M. bovis BCG Moreau Dodecina menos estável Ligação de flavina inferior |
topic |
Perda do Terminal N M. bovis BCG Moreau Dodecina menos estável Ligação de flavina inferior M. bovis BCG Moreau N-Terminal Loss Dodecin Lower Flavin Binding Capacity |
dc.subject.en.pt_BR.fl_str_mv |
M. bovis BCG Moreau N-Terminal Loss Dodecin Lower Flavin Binding Capacity |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-04-12T18:46:49Z |
dc.date.available.fl_str_mv |
2021-04-12T18:46:49Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/46653 |
dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
dc.identifier.doi.none.fl_str_mv |
10.3389/fcimb.2021.658888 |
identifier_str_mv |
SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium tuberculosis and M. bovis BCG Moreau Fumarate Reductase Operons Produce Different Polypeptides That May Be Related to Non-canonical Functions. Front. Microbiol., v. 11, Article 624121, 15p, Jan. 2021. 2235-2988 10.3389/fcimb.2021.658888 |
url |
https://www.arca.fiocruz.br/handle/icict/46653 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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