Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells

Detalhes bibliográficos
Autor(a) principal: Alvarez, Luis Carlos Salazar
Data de Publicação: 2021
Outros Autores: Lizcano, Omaira Vera, Barros, Dayanne Kamylla Alves da Silva, Silva, Djane Clarys Baia da, Monteiro, Wuelton Marcelo, Pimenta, Paulo Filemon Paolluci, Lacerda, Marcus Vinicius Guimarães de, Costa, Fabio Trindade Maranhão, Lopes, Stefanie Costa Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51771
Resumo: Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brazil/Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.
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spelling Alvarez, Luis Carlos SalazarLizcano, Omaira VeraBarros, Dayanne Kamylla Alves da SilvaSilva, Djane Clarys Baia daMonteiro, Wuelton MarceloPimenta, Paulo Filemon PaolluciLacerda, Marcus Vinicius Guimarães deCosta, Fabio Trindade MaranhãoLopes, Stefanie Costa Pinto2022-03-21T15:40:16Z2022-03-21T15:40:16Z2021ALVAREZ, Luis Carlos Salazar et al. Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells. Front Cell Infect Microbiol., v. 11, 614985, 2021. doi: 10.3389/fcimb.2021.614985.2235-2988https://www.arca.fiocruz.br/handle/icict/5177110.3389/fcimb.2021.614985engFrontiers Media ASPlasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brazil/Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/ Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil/ Grupo de Investigación QUIBIO. Facultad de Ciencias Básicas. Universidad Santiago de Cali. Cali, Colombia.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil.Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil.In a Plasmodium vivax infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the ex vivo cytoadhesive capacity of P. vivax gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). Ex-vivo cytoadhesion assays were performed using a total of 44 P. vivax isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte P. vivax adheres ex vivo to bone marrow endothelial cells. Moreover, P. vivax gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a P. vivax reservoir and the potential impact on parasite transmission to the vector.MalariaPlasmodium vivaxbone marrowcytoadhesion; gametocytes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51771/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPlasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells..pdfPlasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells..pdfapplication/pdf3575510https://www.arca.fiocruz.br/bitstream/icict/51771/2/Plasmodium%20vivax%20Gametocytes%20Adherence%20to%20Bone%20Marrow%20Endothelial%20Cells..pdf390e81c3b1f54e2db2407513185a0de4MD52icict/517712022-03-21 12:40:16.041oai:www.arca.fiocruz.br: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Repositório 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dc.title.pt_BR.fl_str_mv Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
title Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
spellingShingle Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
Alvarez, Luis Carlos Salazar
Malaria
Plasmodium vivax
bone marrow
cytoadhesion; gametocytes.
title_short Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
title_full Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
title_fullStr Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
title_full_unstemmed Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
title_sort Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
author Alvarez, Luis Carlos Salazar
author_facet Alvarez, Luis Carlos Salazar
Lizcano, Omaira Vera
Barros, Dayanne Kamylla Alves da Silva
Silva, Djane Clarys Baia da
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolluci
Lacerda, Marcus Vinicius Guimarães de
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
author_role author
author2 Lizcano, Omaira Vera
Barros, Dayanne Kamylla Alves da Silva
Silva, Djane Clarys Baia da
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolluci
Lacerda, Marcus Vinicius Guimarães de
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alvarez, Luis Carlos Salazar
Lizcano, Omaira Vera
Barros, Dayanne Kamylla Alves da Silva
Silva, Djane Clarys Baia da
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolluci
Lacerda, Marcus Vinicius Guimarães de
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
dc.subject.en.pt_BR.fl_str_mv Malaria
Plasmodium vivax
bone marrow
cytoadhesion; gametocytes.
topic Malaria
Plasmodium vivax
bone marrow
cytoadhesion; gametocytes.
description Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brazil/Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-03-21T15:40:16Z
dc.date.available.fl_str_mv 2022-03-21T15:40:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVAREZ, Luis Carlos Salazar et al. Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells. Front Cell Infect Microbiol., v. 11, 614985, 2021. doi: 10.3389/fcimb.2021.614985.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51771
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2021.614985
identifier_str_mv ALVAREZ, Luis Carlos Salazar et al. Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells. Front Cell Infect Microbiol., v. 11, 614985, 2021. doi: 10.3389/fcimb.2021.614985.
2235-2988
10.3389/fcimb.2021.614985
url https://www.arca.fiocruz.br/handle/icict/51771
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media AS
publisher.none.fl_str_mv Frontiers Media AS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51771/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51771/2/Plasmodium%20vivax%20Gametocytes%20Adherence%20to%20Bone%20Marrow%20Endothelial%20Cells..pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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