Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease

Detalhes bibliográficos
Autor(a) principal: Acquarone, Mariana
Data de Publicação: 2015
Outros Autores: Melo, Thiago M. de, Meireles, Fernanda, Moreira, Jordano Brito, Oliveira, Gabriel, Ferreira, Sergio T., Castro, Newton G., Tovar-Moll, Fernanda, Houzel, Jean-Christophe, Rehen, Stevens K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13845
Resumo: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.
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spelling Acquarone, MarianaMelo, Thiago M. deMeireles, FernandaMoreira, Jordano BritoOliveira, GabrielFerreira, Sergio T.Castro, Newton G.Tovar-Moll, FernandaHouzel, Jean-ChristopheRehen, Stevens K.2016-04-18T14:20:55Z2016-04-18T14:20:55Z2015ACQUARONE, Mariana; et al. Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease. Frontiers in Cellular Neuroscience, v.9, Article 97, 12p, Apr. 2015.https://www.arca.fiocruz.br/handle/icict/1384510.3389/fncel.2015.000971662-5102engFrontiers MediaMitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.Instituto D`Or de Pesquisa e Educação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil / Instituto D`Or de Pesquisa e Educação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil / Instituto D`Or de Pesquisa e Educação. Rio de Janeiro, RJ, Brasil.Parkinson’s disease (PD) is an incurable progressive neurodegenerative disorder. Clinical presentation of PD stems largely from the loss of dopaminergic neurons in the nigrostriatal dopaminergic pathway, motivating experimental strategies of replacement based on cell therapy. Transplantation of dopaminergic neurons derived from embryonic stem cells significantly improves motor functions in rodent and non-human primate models of PD. However, protocols to generate dopaminergic neurons from embryonic stem cells generally meet with low efficacy and high risk of teratoma formation upon transplantation. To address these issues, we have pre-treated undifferentiated mouse embryonic stem cells (mESCs) with the DNA alkylating agent mitomycin C (MMC) before transplantation. MMC treatment of cultures prevented tumorigenesis in a 12 week follow-up after mESCs were injected in nude mice. In 6-OH-dopamine-lesioned mice, intrastriatal injection of MMC-treated mESCs markedly improved motor function without tumor formation for as long as 15 months. Furthermore, we show that halting mitotic activity of undifferentiated mESCs induces a four-fold increase in dopamine release following in vitro differentiation. Our findings indicate that treating mESCs with MMC prior to intrastriatal transplant is an effective to strategy that could be further investigated as a novel alternative for treatment of PD.Embryonic stem cellStem cell therapyParkinson modelMitomycin CContrast-enhanced MRIMiceMitomicinaDoença de ParkinsonImagem por Ressonância MagnéticaCélulas-TroncoTerapiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13845/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgabriel_oliveira_etal_IOC_2015.pdfgabriel_oliveira_etal_IOC_2015.pdfapplication/pdf2045662https://www.arca.fiocruz.br/bitstream/icict/13845/2/gabriel_oliveira_etal_IOC_2015.pdf8b60ebdede62c9d97710ca34971463a1MD52TEXTgabriel_oliveira_etal_IOC_2015.pdf.txtgabriel_oliveira_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
title Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
spellingShingle Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
Acquarone, Mariana
Embryonic stem cell
Stem cell therapy
Parkinson model
Mitomycin C
Contrast-enhanced MRI
Mice
Mitomicina
Doença de Parkinson
Imagem por Ressonância Magnética
Células-Tronco
Terapia
title_short Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
title_full Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
title_fullStr Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
title_full_unstemmed Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
title_sort Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
author Acquarone, Mariana
author_facet Acquarone, Mariana
Melo, Thiago M. de
Meireles, Fernanda
Moreira, Jordano Brito
Oliveira, Gabriel
Ferreira, Sergio T.
Castro, Newton G.
Tovar-Moll, Fernanda
Houzel, Jean-Christophe
Rehen, Stevens K.
author_role author
author2 Melo, Thiago M. de
Meireles, Fernanda
Moreira, Jordano Brito
Oliveira, Gabriel
Ferreira, Sergio T.
Castro, Newton G.
Tovar-Moll, Fernanda
Houzel, Jean-Christophe
Rehen, Stevens K.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Acquarone, Mariana
Melo, Thiago M. de
Meireles, Fernanda
Moreira, Jordano Brito
Oliveira, Gabriel
Ferreira, Sergio T.
Castro, Newton G.
Tovar-Moll, Fernanda
Houzel, Jean-Christophe
Rehen, Stevens K.
dc.subject.en.pt_BR.fl_str_mv Embryonic stem cell
Stem cell therapy
Parkinson model
Mitomycin C
Contrast-enhanced MRI
Mice
topic Embryonic stem cell
Stem cell therapy
Parkinson model
Mitomycin C
Contrast-enhanced MRI
Mice
Mitomicina
Doença de Parkinson
Imagem por Ressonância Magnética
Células-Tronco
Terapia
dc.subject.decs.pt_BR.fl_str_mv Mitomicina
Doença de Parkinson
Imagem por Ressonância Magnética
Células-Tronco
Terapia
description Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biociências. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-18T14:20:55Z
dc.date.available.fl_str_mv 2016-04-18T14:20:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ACQUARONE, Mariana; et al. Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease. Frontiers in Cellular Neuroscience, v.9, Article 97, 12p, Apr. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13845
dc.identifier.doi.none.fl_str_mv 10.3389/fncel.2015.00097
dc.identifier.eissn.none.fl_str_mv 1662-5102
identifier_str_mv ACQUARONE, Mariana; et al. Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease. Frontiers in Cellular Neuroscience, v.9, Article 97, 12p, Apr. 2015.
10.3389/fncel.2015.00097
1662-5102
url https://www.arca.fiocruz.br/handle/icict/13845
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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