CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/9164 |
Resumo: | The transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC. |
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CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cellsTranscriptional regulationCardiac progenitorCited2Isl1Embryonic stem cellDifferentiationThe transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC.ElsevierSapientiaPacheco-Leyva, IvetteMatias, Ana CatarinaOliveira, Daniel V.Santos, JoãoNascimento, RitaGuerreiro, EduardaMichell, Anna C.van De Vrugt, Annebel M.Machado-Oliveira, GiselaFerreira, GuilhermeDomian, IbrahimBragança, José2017-04-07T15:55:34Z2016-122016-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/9164eng2213-6711AUT: JEB02317;https://doi.org/10.1016/j.stemcr.2016.10.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:20:34Zoai:sapientia.ualg.pt:10400.1/9164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:01:11.105801Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
title |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
spellingShingle |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells Pacheco-Leyva, Ivette Transcriptional regulation Cardiac progenitor Cited2 Isl1 Embryonic stem cell Differentiation |
title_short |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
title_full |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
title_fullStr |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
title_full_unstemmed |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
title_sort |
CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells |
author |
Pacheco-Leyva, Ivette |
author_facet |
Pacheco-Leyva, Ivette Matias, Ana Catarina Oliveira, Daniel V. Santos, João Nascimento, Rita Guerreiro, Eduarda Michell, Anna C. van De Vrugt, Annebel M. Machado-Oliveira, Gisela Ferreira, Guilherme Domian, Ibrahim Bragança, José |
author_role |
author |
author2 |
Matias, Ana Catarina Oliveira, Daniel V. Santos, João Nascimento, Rita Guerreiro, Eduarda Michell, Anna C. van De Vrugt, Annebel M. Machado-Oliveira, Gisela Ferreira, Guilherme Domian, Ibrahim Bragança, José |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Pacheco-Leyva, Ivette Matias, Ana Catarina Oliveira, Daniel V. Santos, João Nascimento, Rita Guerreiro, Eduarda Michell, Anna C. van De Vrugt, Annebel M. Machado-Oliveira, Gisela Ferreira, Guilherme Domian, Ibrahim Bragança, José |
dc.subject.por.fl_str_mv |
Transcriptional regulation Cardiac progenitor Cited2 Isl1 Embryonic stem cell Differentiation |
topic |
Transcriptional regulation Cardiac progenitor Cited2 Isl1 Embryonic stem cell Differentiation |
description |
The transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12 2016-12-01T00:00:00Z 2017-04-07T15:55:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/9164 |
url |
http://hdl.handle.net/10400.1/9164 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2213-6711 AUT: JEB02317; https://doi.org/10.1016/j.stemcr.2016.10.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133240237228032 |