CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells

Detalhes bibliográficos
Autor(a) principal: Pacheco-Leyva, Ivette
Data de Publicação: 2016
Outros Autores: Matias, Ana Catarina, Oliveira, Daniel V., Santos, João, Nascimento, Rita, Guerreiro, Eduarda, Michell, Anna C., van De Vrugt, Annebel M., Machado-Oliveira, Gisela, Ferreira, Guilherme, Domian, Ibrahim, Bragança, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/9164
Resumo: The transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC.
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spelling CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cellsTranscriptional regulationCardiac progenitorCited2Isl1Embryonic stem cellDifferentiationThe transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC.ElsevierSapientiaPacheco-Leyva, IvetteMatias, Ana CatarinaOliveira, Daniel V.Santos, JoãoNascimento, RitaGuerreiro, EduardaMichell, Anna C.van De Vrugt, Annebel M.Machado-Oliveira, GiselaFerreira, GuilhermeDomian, IbrahimBragança, José2017-04-07T15:55:34Z2016-122016-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/9164eng2213-6711AUT: JEB02317;https://doi.org/10.1016/j.stemcr.2016.10.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:20:34Zoai:sapientia.ualg.pt:10400.1/9164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:01:11.105801Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
title CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
spellingShingle CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
Pacheco-Leyva, Ivette
Transcriptional regulation
Cardiac progenitor
Cited2
Isl1
Embryonic stem cell
Differentiation
title_short CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
title_full CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
title_fullStr CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
title_full_unstemmed CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
title_sort CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
author Pacheco-Leyva, Ivette
author_facet Pacheco-Leyva, Ivette
Matias, Ana Catarina
Oliveira, Daniel V.
Santos, João
Nascimento, Rita
Guerreiro, Eduarda
Michell, Anna C.
van De Vrugt, Annebel M.
Machado-Oliveira, Gisela
Ferreira, Guilherme
Domian, Ibrahim
Bragança, José
author_role author
author2 Matias, Ana Catarina
Oliveira, Daniel V.
Santos, João
Nascimento, Rita
Guerreiro, Eduarda
Michell, Anna C.
van De Vrugt, Annebel M.
Machado-Oliveira, Gisela
Ferreira, Guilherme
Domian, Ibrahim
Bragança, José
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Pacheco-Leyva, Ivette
Matias, Ana Catarina
Oliveira, Daniel V.
Santos, João
Nascimento, Rita
Guerreiro, Eduarda
Michell, Anna C.
van De Vrugt, Annebel M.
Machado-Oliveira, Gisela
Ferreira, Guilherme
Domian, Ibrahim
Bragança, José
dc.subject.por.fl_str_mv Transcriptional regulation
Cardiac progenitor
Cited2
Isl1
Embryonic stem cell
Differentiation
topic Transcriptional regulation
Cardiac progenitor
Cited2
Isl1
Embryonic stem cell
Differentiation
description The transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2016-12-01T00:00:00Z
2017-04-07T15:55:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/9164
url http://hdl.handle.net/10400.1/9164
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2213-6711
AUT: JEB02317;
https://doi.org/10.1016/j.stemcr.2016.10.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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