DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells

Detalhes bibliográficos
Autor(a) principal: Inácio, José Manuel
Data de Publicação: 2021
Outros Autores: von Gilsa Lopes, João, Silva, Ana Mafalda, Cristo, Fernando, Marques, Sara, Futschik, Matthias E., Belo, José António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120670
Resumo: Funding: This work was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BIM-MED/3363/2014) and Scientific Employment Stimulus to JI (Norma Transitória 8189/2018), predoctoral fellowship to JG (FCT; PD/BD/136919/2018) and postdoctoral fellowship to FC (DAI/ 2019/08/SAICTPAC/0047/2015), and iNOVA4Health-UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e a Tecnologia/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.
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spelling DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cellscardiac differentiationcardiac progenitor cellcardiomyocyte proliferationDand5embryonic stem cellsDevelopmental BiologyCell BiologyFunding: This work was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BIM-MED/3363/2014) and Scientific Employment Stimulus to JI (Norma Transitória 8189/2018), predoctoral fellowship to JG (FCT; PD/BD/136919/2018) and postdoctoral fellowship to FC (DAI/ 2019/08/SAICTPAC/0047/2015), and iNOVA4Health-UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e a Tecnologia/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.Deciphering the clues of a regenerative mechanism for the mammalian adult heart would save millions of lives in the near future. Heart failure due to cardiomyocyte loss is still one of the significant health burdens worldwide. Here, we show the potential of a single molecule, DAND5, in mouse pluripotent stem cell-derived cardiomyocytes specification and proliferation. Dand5 loss-of-function generated the double of cardiac beating foci compared to the wild-type cells. The early formation of cardiac progenitor cells and the increased proliferative capacity of Dand5 KO mESC-derived cardiomyocytes contribute to the observed higher number of derived cardiac cells. Transcriptional profiling sequencing and quantitative RT-PCR assays showed an upregulation of early cardiac gene networks governing cardiomyocyte differentiation, cell cycling, and cardiac regenerative pathways but reduced levels of genes involved in cardiomyocyte maturation. These findings prompt DAND5 as a key driver for the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems with further clinical application purposes.iNOVA4Health - pólo NMSCentro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNInácio, José Manuelvon Gilsa Lopes, JoãoSilva, Ana MafaldaCristo, FernandoMarques, SaraFutschik, Matthias E.Belo, José António2021-07-07T22:18:52Z2021-04-132021-04-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120670eng2296-634XPURE: 29658321https://doi.org/10.3389/fcell.2021.629430info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:03:08Zoai:run.unl.pt:10362/120670Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:24.854760Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
title DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
spellingShingle DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
Inácio, José Manuel
cardiac differentiation
cardiac progenitor cell
cardiomyocyte proliferation
Dand5
embryonic stem cells
Developmental Biology
Cell Biology
title_short DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
title_full DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
title_fullStr DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
title_full_unstemmed DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
title_sort DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells
author Inácio, José Manuel
author_facet Inácio, José Manuel
von Gilsa Lopes, João
Silva, Ana Mafalda
Cristo, Fernando
Marques, Sara
Futschik, Matthias E.
Belo, José António
author_role author
author2 von Gilsa Lopes, João
Silva, Ana Mafalda
Cristo, Fernando
Marques, Sara
Futschik, Matthias E.
Belo, José António
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv iNOVA4Health - pólo NMS
Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Inácio, José Manuel
von Gilsa Lopes, João
Silva, Ana Mafalda
Cristo, Fernando
Marques, Sara
Futschik, Matthias E.
Belo, José António
dc.subject.por.fl_str_mv cardiac differentiation
cardiac progenitor cell
cardiomyocyte proliferation
Dand5
embryonic stem cells
Developmental Biology
Cell Biology
topic cardiac differentiation
cardiac progenitor cell
cardiomyocyte proliferation
Dand5
embryonic stem cells
Developmental Biology
Cell Biology
description Funding: This work was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BIM-MED/3363/2014) and Scientific Employment Stimulus to JI (Norma Transitória 8189/2018), predoctoral fellowship to JG (FCT; PD/BD/136919/2018) and postdoctoral fellowship to FC (DAI/ 2019/08/SAICTPAC/0047/2015), and iNOVA4Health-UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e a Tecnologia/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-07T22:18:52Z
2021-04-13
2021-04-13T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120670
url http://hdl.handle.net/10362/120670
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-634X
PURE: 29658321
https://doi.org/10.3389/fcell.2021.629430
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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