Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo

Fox, James M; Hilburn, Silva; Demontis, Maria-Antonietta; Brighty, David W; Grassi, Maria Fernanda Rios; Castro Filho, Bernardo Galvão; Taylor, Graham P; Martin, Fabiola

Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo

Detalhes bibliográficos
Autor(a) principal:	Fox, James M
Data de Publicação:	2016
Outros Autores:	Hilburn, Silva, Demontis, Maria-Antonietta, Brighty, David W, Grassi, Maria Fernanda Rios, Castro Filho, Bernardo Galvão, Taylor, Graham P, Martin, Fabiola
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/14305
Resumo:	University of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UK

Metadados do item

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spelling	Fox, James MHilburn, SilvaDemontis, Maria-AntoniettaBrighty, David WGrassi, Maria Fernanda RiosCastro Filho, Bernardo GalvãoTaylor, Graham PMartin, Fabiola2016-05-17T12:38:02Z2016-05-17T12:38:02Z2016FOX, J. M. et al. Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo. Viruses, v. 8, p. 80, 2016.1999-491510.3390/v8030080https://www.arca.fiocruz.br/handle/icict/14305engMDPILong Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UKImperial College. Section of Infectious Diseases. Department of Medicine. London, UKImperial College. Section of Infectious Diseases. Department of Medicine. London, UK / The National Centre for Human Retrovirology/HTLV Clinic. Imperial College Healthcare NHS Trust St Mary’s Hospital. London NY, UKUniversity of Dundee. Division of Cancer Research. Ninewells Hospital & Medical School. Dundee, UKFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilHTLV-Centre Bahian School of Medicine. Salvador, Bahia, BrasilImperial College. Section of Infectious Diseases. Department of Medicine. London, UK / The National Centre for Human Retrovirology/HTLV Clinic. Imperial College Healthcare NHS Trust St Mary’s Hospital. London NY, UKUniversity of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UKClonal expansion of human T-lymphotropic virus type-1 (HTLV-1) infected cells in vivo is well documented. Unlike human immunodeficiency virus type 1 (HIV-1), HTLV-1 plasma RNA is sparse. The contribution of the “mitotic” spread of HTLV-1 compared with infectious spread of the virus to HTLV-1 viral burden in established infection is uncertain. Since extrachromosomal long terminal repeat (LTR) DNA circles are indicators of viral replication in HIV-1 carriers with undetectable plasma HIV RNA, we hypothesised that HTLV-1 LTR circles could indicate reverse transcriptase (RT) usage and infectious activity. 1LTR and 2LTR DNA circles were measured in HTLV-1 cell lines and peripheral blood mononuclear cells (PBMC) of asymptomatic carriers (ACs) and patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) or adult T cell leukaemia/lymphoma (ATLL). 1LTR DNA circles were detected in 14/20 patients at a mean of 1.38/100 PBMC but did not differentiate disease status nor correlate with HTLV-1 DNA copies. 2LTR DNA circles were detected in 30/31 patients and at higher concentrations in patients with HTLV-1-associated diseases, independent of HTLV-1 DNA load. In an incident case the 2LTR DNA circle concentration increased 2.1 fold at the onset of HAM/TSP compared to baseline. Detectable and fluctuating levels of HTLV-1 DNA circles in patients indicate viral RT usage and virus replication. Our results indicate HTLV-1 viral replication capacity is maintained in chronic infection and may be associated with disease onset.Long terminal repeatLTR DNA circlesHuman T-lymphotropic virusHTLVViral replicationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14305/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFox JM Long terminal....pdfFox JM Long terminal....pdfapplication/pdf999970https://www.arca.fiocruz.br/bitstream/icict/14305/2/Fox%20JM%20Long%20terminal....pdfa45deb5982c560ac3c9469f34ec12755MD52TEXTFox JM Long terminal....pdf.txtFox JM Long terminal....pdf.txtExtracted texttext/plain55119https://www.arca.fiocruz.br/bitstream/icict/14305/3/Fox%20JM%20Long%20terminal....pdf.txt5eddb7e8e4e5261c5eec51c26d1ff971MD53icict/143052023-03-15 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dc.title.pt_BR.fl_str_mv	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
title	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
spellingShingle	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo Fox, James M Long terminal repeat LTR DNA circles Human T-lymphotropic virus HTLV Viral replication
title_short	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
title_full	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
title_fullStr	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
title_full_unstemmed	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
title_sort	Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo
author	Fox, James M
author_facet	Fox, James M Hilburn, Silva Demontis, Maria-Antonietta Brighty, David W Grassi, Maria Fernanda Rios Castro Filho, Bernardo Galvão Taylor, Graham P Martin, Fabiola
author_role	author
author2	Hilburn, Silva Demontis, Maria-Antonietta Brighty, David W Grassi, Maria Fernanda Rios Castro Filho, Bernardo Galvão Taylor, Graham P Martin, Fabiola
author2_role	author author author author author author author
dc.contributor.author.fl_str_mv	Fox, James M Hilburn, Silva Demontis, Maria-Antonietta Brighty, David W Grassi, Maria Fernanda Rios Castro Filho, Bernardo Galvão Taylor, Graham P Martin, Fabiola
dc.subject.en.pt_BR.fl_str_mv	Long terminal repeat LTR DNA circles Human T-lymphotropic virus HTLV Viral replication
topic	Long terminal repeat LTR DNA circles Human T-lymphotropic virus HTLV Viral replication
description	University of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UK
publishDate	2016
dc.date.accessioned.fl_str_mv	2016-05-17T12:38:02Z
dc.date.available.fl_str_mv	2016-05-17T12:38:02Z
dc.date.issued.fl_str_mv	2016
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	FOX, J. M. et al. Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo. Viruses, v. 8, p. 80, 2016.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/14305
dc.identifier.issn.none.fl_str_mv	1999-4915 10.3390/v8030080
identifier_str_mv	FOX, J. M. et al. Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo. Viruses, v. 8, p. 80, 2016. 1999-4915 10.3390/v8030080
url	https://www.arca.fiocruz.br/handle/icict/14305
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/14305/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/14305/2/Fox%20JM%20Long%20terminal....pdf https://www.arca.fiocruz.br/bitstream/icict/14305/3/Fox%20JM%20Long%20terminal....pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo

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