In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9

Detalhes bibliográficos
Autor(a) principal: Silva, Rodrigo Nunes Rodrigues da
Data de Publicação: 2016
Outros Autores: Silva, João Hermínio Martins da, Singh, Balwan, Jiang, Jianlin, Meyer, Esmeralda V. S., Santos, Fátima, Banic, Dalma Maria, Moreno, Alberto, Galinski, Mary R., Ferreira, Joseli Oliveira, Lima Junior, Josué da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14428
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Rodrigo Nunes Rodrigues daSilva, João Hermínio Martins daSingh, BalwanJiang, JianlinMeyer, Esmeralda V. S.Santos, FátimaBanic, Dalma MariaMoreno, AlbertoGalinski, Mary R.Ferreira, Joseli OliveiraLima Junior, Josué da Costa2016-06-02T12:56:12Z2016-06-03T12:34:16Z2016-06-02T12:56:12Z2016-06-03T12:34:16Z2016SILVA, Rodrigo Nunes Rodrigues da; et al. In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9. Plos One, v.11, n.1, e0146951, 18p, Jan. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1442810.1371/journal.pone.0146951engPublic Library of ScienceEpitopo de célula linear BMaláriaPlasmodium vivaxVacinaProteína 9 de Superfície de MerozoitoPlasmodium vivaxMalariaVaccinelinear B cell epitopeMerozoite Surface Protein-9In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Grupo de Modelagem Computacional. Fortaleza, CE, Brasil.Emory University. Yerkes National Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA.Emory University. Yerkes National Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA.Emory University. Environmental Health and Safety Office. Atlanta, GA, USA.Fundação Nacional de Saúde. Departamento de Entomologia. Laboratório central. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Referência Nacional de Simulídeos e Oncocercose. Rio de Janeiro, RJ, Brasil.Emory University. Yerkes National Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA / Emory University. Emory University School of Medicine. Department of Medicine. Division of Infectious Diseases. Atlanta, GA, USA.Emory University. Yerkes National Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA / Emory University. Emory University School of Medicine. Department of Medicine. Division of Infectious Diseases. Atlanta, GA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Synthetic peptide vaccines provide the advantages of safety, stability and low cost. The success of this approach is highly dependent on efficient epitope identification and synthetic strategies for efficacious delivery. In malaria, the Merozoite Surface Protein-9 of Plasmodium vivax (PvMSP9) has been considered a vaccine candidate based on the evidence that specific antibodies were able to inhibit merozoite invasion and recombinant proteins were highly immunogenic in mice and humans. However the identities of linear B-cell epitopes within PvMSP9 as targets of functional antibodies remain undefined. We used several publicly- available algorithms for in silico analyses and prediction of relevant B cell epitopes within PMSP9.We show that the tandem repeat sequence EAAPENAEPVHENA (PvMSP9E795-A808) present at the C-terminal region is a promising target for antibodies, given its high combined score to be a linear epitope and located in a putative intrinsically unstructured region of the native protein. To confirm the predictive value of the computational approach, plasma samples from 545 naturally exposed individuals were screened for IgG reactivity against the recombinant PvMSP9-RIRII729-972 and a synthetic peptide representing the predicted B cell epitope PvMSP9E795-A808. 316 individuals (58%) were responders to the full repetitive region PvMSP9-RIRII, of which 177 (56%) also presented total IgG reactivity against the synthetic peptide, confirming it validity as a B cell epitope. The reactivity indexes of anti-PvMSP9-RIRII and anti-PvMSP9E795-A808 antibodies were correlated. Interestingly, a potential role in the acquisition of protective immunity was associated with the linear epitope, since the IgG1 subclass against PvMSP9E795-A808 was the prevalent subclass and this directly correlated with time elapsed since the last malaria episode; however this was not observed in the antibody responses against the full PvMSP9-RIRII. In conclusion, our findings identified and experimentally confirmed the potential of PvMSP9E795-A808 as an immunogenic linear B cell epitope within the P. vivax malaria vaccine candidate PvMSP9 and support its inclusion in future subunit vaccines.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALdalma_banic_etal_IOC_2016.PDFapplication/pdf1194916https://www.arca.fiocruz.br/bitstream/icict/14428/1/dalma_banic_etal_IOC_2016.PDFb3b7e7ecb81de1803e07ac4391fc1c17MD51LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14428/2/license.txt5a560609d32a3863062d77ff32785d58MD52TEXTdalma_banic_etal_IOC_2016.PDF.txtdalma_banic_etal_IOC_2016.PDF.txtExtracted texttext/plain71841https://www.arca.fiocruz.br/bitstream/icict/14428/3/dalma_banic_etal_IOC_2016.PDF.txtcbfc014558710de94008a6e051979623MD53icict/144282022-06-24 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dc.title.pt_BR.fl_str_mv In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
title In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
spellingShingle In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
Silva, Rodrigo Nunes Rodrigues da
Epitopo de célula linear B
Malária
Plasmodium vivax
Vacina
Proteína 9 de Superfície de Merozoito
Plasmodium vivax
Malaria
Vaccine
linear B cell epitope
Merozoite Surface Protein-9
title_short In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
title_full In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
title_fullStr In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
title_full_unstemmed In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
title_sort In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
author Silva, Rodrigo Nunes Rodrigues da
author_facet Silva, Rodrigo Nunes Rodrigues da
Silva, João Hermínio Martins da
Singh, Balwan
Jiang, Jianlin
Meyer, Esmeralda V. S.
Santos, Fátima
Banic, Dalma Maria
Moreno, Alberto
Galinski, Mary R.
Ferreira, Joseli Oliveira
Lima Junior, Josué da Costa
author_role author
author2 Silva, João Hermínio Martins da
Singh, Balwan
Jiang, Jianlin
Meyer, Esmeralda V. S.
Santos, Fátima
Banic, Dalma Maria
Moreno, Alberto
Galinski, Mary R.
Ferreira, Joseli Oliveira
Lima Junior, Josué da Costa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Rodrigo Nunes Rodrigues da
Silva, João Hermínio Martins da
Singh, Balwan
Jiang, Jianlin
Meyer, Esmeralda V. S.
Santos, Fátima
Banic, Dalma Maria
Moreno, Alberto
Galinski, Mary R.
Ferreira, Joseli Oliveira
Lima Junior, Josué da Costa
dc.subject.other.pt_BR.fl_str_mv Epitopo de célula linear B
Malária
Plasmodium vivax
Vacina
Proteína 9 de Superfície de Merozoito
topic Epitopo de célula linear B
Malária
Plasmodium vivax
Vacina
Proteína 9 de Superfície de Merozoito
Plasmodium vivax
Malaria
Vaccine
linear B cell epitope
Merozoite Surface Protein-9
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Malaria
Vaccine
linear B cell epitope
Merozoite Surface Protein-9
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-02T12:56:12Z
2016-06-03T12:34:16Z
dc.date.available.fl_str_mv 2016-06-02T12:56:12Z
2016-06-03T12:34:16Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Rodrigo Nunes Rodrigues da; et al. In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9. Plos One, v.11, n.1, e0146951, 18p, Jan. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14428
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0146951
identifier_str_mv SILVA, Rodrigo Nunes Rodrigues da; et al. In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9. Plos One, v.11, n.1, e0146951, 18p, Jan. 2016.
1932-6203
10.1371/journal.pone.0146951
url https://www.arca.fiocruz.br/handle/icict/14428
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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