Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Detalhes bibliográficos
Autor(a) principal: Gomes, Luciana Inácia
Data de Publicação: 2009
Outros Autores: Marques, Letícia Helena dos Santos, Enk, Martin Johannes, Coelho, Paulo Marcos Zech, Rabello, Ana Lucia Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58794
https://doi.org/10.1590/s0074-02762009000800021
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
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spelling Gomes, Luciana InáciaMarques, Letícia Helena dos SantosEnk, Martin JohannesCoelho, Paulo Marcos ZechRabello, Ana Lucia Teles2023-05-29T13:53:33Z2023-05-29T13:53:33Z2009GOMES, Luciana Inácia et al. Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Mem Inst Oswaldo Cruz, v.104, n. 8, p. 1194-1196, 2009. doi: 10.1590/s0074-02762009000800021.0074-0276https://www.arca.fiocruz.br/handle/icict/58794https://doi.org/10.1590/s0074-02762009000800021engMinistério da SaúdeFurther evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, BrasilA previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settingsschistosomiasismolecular diagnosisfaecal samplespolymerase chain reactioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALindex.pdfindex.pdfapplication/pdf94312https://www.arca.fiocruz.br/bitstream/icict/58794/2/index.pdf55f2399af47f7898de0a86f2aedaec8dMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58794/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/587942023-05-29 10:53:34.941oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-29T13:53:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
spellingShingle Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
Gomes, Luciana Inácia
schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
title_short Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_full Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_fullStr Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_full_unstemmed Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_sort Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
author Gomes, Luciana Inácia
author_facet Gomes, Luciana Inácia
Marques, Letícia Helena dos Santos
Enk, Martin Johannes
Coelho, Paulo Marcos Zech
Rabello, Ana Lucia Teles
author_role author
author2 Marques, Letícia Helena dos Santos
Enk, Martin Johannes
Coelho, Paulo Marcos Zech
Rabello, Ana Lucia Teles
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Luciana Inácia
Marques, Letícia Helena dos Santos
Enk, Martin Johannes
Coelho, Paulo Marcos Zech
Rabello, Ana Lucia Teles
dc.subject.en.en_US.fl_str_mv schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
topic schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2023-05-29T13:53:33Z
dc.date.available.fl_str_mv 2023-05-29T13:53:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, Luciana Inácia et al. Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Mem Inst Oswaldo Cruz, v.104, n. 8, p. 1194-1196, 2009. doi: 10.1590/s0074-02762009000800021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58794
dc.identifier.issn.en_US.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1590/s0074-02762009000800021
identifier_str_mv GOMES, Luciana Inácia et al. Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Mem Inst Oswaldo Cruz, v.104, n. 8, p. 1194-1196, 2009. doi: 10.1590/s0074-02762009000800021.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/58794
https://doi.org/10.1590/s0074-02762009000800021
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Ministério da Saúde
publisher.none.fl_str_mv Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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