Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Detalhes bibliográficos
Autor(a) principal: Gomes,Luciana I
Data de Publicação: 2009
Outros Autores: Marques,Letícia HS, Enk,Martin J, Coelho,Paulo Marcos Z, Rabello,Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800021
Resumo: A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
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spelling Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samplesschistosomiasismolecular diagnosisfaecal samplespolymerase chain reactionA previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.Instituto Oswaldo Cruz, Ministério da Saúde2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800021Memórias do Instituto Oswaldo Cruz v.104 n.8 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000800021info:eu-repo/semantics/openAccessGomes,Luciana IMarques,Letícia HSEnk,Martin JCoelho,Paulo Marcos ZRabello,Anaeng2020-04-25T17:50:36Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:35.505Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
spellingShingle Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
Gomes,Luciana I
schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
title_short Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_full Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_fullStr Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_full_unstemmed Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
title_sort Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
author Gomes,Luciana I
author_facet Gomes,Luciana I
Marques,Letícia HS
Enk,Martin J
Coelho,Paulo Marcos Z
Rabello,Ana
author_role author
author2 Marques,Letícia HS
Enk,Martin J
Coelho,Paulo Marcos Z
Rabello,Ana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gomes,Luciana I
Marques,Letícia HS
Enk,Martin J
Coelho,Paulo Marcos Z
Rabello,Ana
dc.subject.por.fl_str_mv schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
topic schistosomiasis
molecular diagnosis
faecal samples
polymerase chain reaction
dc.description.none.fl_txt_mv A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
description A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800021
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762009000800021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.104 n.8 2009
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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