High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/38911 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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Barreto, Emiliano O.Carvalho, Vinícius F.Oliveira, Michelle S.Bertho, Alvaro L.Barja-Fidalgo, Thereza C.Cordeiro, Renato S. B.Martins, Marco A.Silva, Patrícia M. R. e2019-12-31T18:13:22Z2019-12-31T18:13:22Z2006BARRETO, Emiliano O. et al. High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes. Life Sciences, v. 79, p. 1194-1202, 2006.0024-3205https://www.arca.fiocruz.br/handle/icict/3891110.1016/j.lfs.2006.03.032engUniversitas Negeri SemarangDiabetesMastócitosApoptoseGlucocorticóidesMicrofilamento de actinaDiabetesMast cellsApoptosisActin microfilamentGlucorticoidsHigh levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Farmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Mast cell number and reactivity were shown to be down-regulated under diabetic conditions. Since the balance between globular and filamentous actin plays a pivotal role in the activity of secretory cells, we investigated whether an imbalance in that system could underlie the hyporesponsiveness of mast cells in diabetes. The apoptotic state was also evaluated. By means of rhodamine/phalloidine staining of F-actin, we noted that diabetic mast cells exhibited an increase in fluorescence intensity and reduction in cellular size, when compared with cells from normal animals, in parallel with elevation in the percentage of cells developing apoptosis. The levels of Bax, a pro-apoptotic member of Bcl-2 family, appeared increased at baseline in mast cells from diabetic rats compared with normal cells. These phenomena correlated with reduction in histamine and PGD2 release following antigen challenge in vitro. The steroid antagonist RU 486 abolished the reduction of histamine secretion from diabetic mast cells. We conclude that hyporesponsiveness of mast cells noted in diabetes may be accounted for by reduction in actin filament plasticity, in clear association with the rise in the percentage of cells undergoing apoptosis. In addition, the refractoriness of diabetic mast cells to antigen in vitro seems to be dependent on glucocorticoids.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/38911/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEmilianoBarreto_etal_IOC_2006.pdfEmilianoBarreto_etal_IOC_2006.pdfapplication/pdf458212https://www.arca.fiocruz.br/bitstream/icict/38911/2/EmilianoBarreto_etal_IOC_2006.pdfe9aae2b07e337f975cfe1755124fd360MD52TEXTEmilianoBarreto_etal_IOC_2006.pdf.txtEmilianoBarreto_etal_IOC_2006.pdf.txtExtracted texttext/plain39584https://www.arca.fiocruz.br/bitstream/icict/38911/3/EmilianoBarreto_etal_IOC_2006.pdf.txt33b75ef57d56f4a9cff29f185773cd72MD53icict/389112020-01-01 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| dc.title.pt_BR.fl_str_mv |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| title |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| spellingShingle |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes Barreto, Emiliano O. Diabetes Mastócitos Apoptose Glucocorticóides Microfilamento de actina Diabetes Mast cells Apoptosis Actin microfilament Glucorticoids |
| title_short |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| title_full |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| title_fullStr |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| title_full_unstemmed |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| title_sort |
High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes |
| author |
Barreto, Emiliano O. |
| author_facet |
Barreto, Emiliano O. Carvalho, Vinícius F. Oliveira, Michelle S. Bertho, Alvaro L. Barja-Fidalgo, Thereza C. Cordeiro, Renato S. B. Martins, Marco A. Silva, Patrícia M. R. e |
| author_role |
author |
| author2 |
Carvalho, Vinícius F. Oliveira, Michelle S. Bertho, Alvaro L. Barja-Fidalgo, Thereza C. Cordeiro, Renato S. B. Martins, Marco A. Silva, Patrícia M. R. e |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barreto, Emiliano O. Carvalho, Vinícius F. Oliveira, Michelle S. Bertho, Alvaro L. Barja-Fidalgo, Thereza C. Cordeiro, Renato S. B. Martins, Marco A. Silva, Patrícia M. R. e |
| dc.subject.other.pt_BR.fl_str_mv |
Diabetes Mastócitos Apoptose Glucocorticóides Microfilamento de actina |
| topic |
Diabetes Mastócitos Apoptose Glucocorticóides Microfilamento de actina Diabetes Mast cells Apoptosis Actin microfilament Glucorticoids |
| dc.subject.en.pt_BR.fl_str_mv |
Diabetes Mast cells Apoptosis Actin microfilament Glucorticoids |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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2006 |
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2006 |
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2019-12-31T18:13:22Z |
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2019-12-31T18:13:22Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BARRETO, Emiliano O. et al. High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes. Life Sciences, v. 79, p. 1194-1202, 2006. |
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https://www.arca.fiocruz.br/handle/icict/38911 |
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0024-3205 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.lfs.2006.03.032 |
| identifier_str_mv |
BARRETO, Emiliano O. et al. High levels of filamentous actin and apoptosis correlate with mast cell refractoriness under alloxan-evoked diabetes. Life Sciences, v. 79, p. 1194-1202, 2006. 0024-3205 10.1016/j.lfs.2006.03.032 |
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Universitas Negeri Semarang |
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Universitas Negeri Semarang |
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