Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.

Detalhes bibliográficos
Autor(a) principal: Stahl, Philipp
Data de Publicação: 2013
Outros Autores: Ruppert, Volker, Meyer, Thomas, Schmidt, Jorg, Campos, Marco Antonio, Gazzinelli, Ricardo Tostes, Maisch, Bernhard, Schwarz, Ralph T., Debierre-Grockiego, Francoise
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30195
Resumo: 2020-01-01
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spelling Stahl, PhilippRuppert, VolkerMeyer, ThomasSchmidt, JorgCampos, Marco AntonioGazzinelli, Ricardo TostesMaisch, BernhardSchwarz, Ralph T.Debierre-Grockiego, Francoise2018-11-26T16:06:06Z2018-11-26T16:06:06Z2013STAHL, Philipp et al. Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro. Apoptosis., v. 18, n. 6, p. 653-63, 20131360-8185https://www.arca.fiocruz.br/handle/icict/3019510.1007/s10495-013-0822-xengSpringerTrypanosoma cruzitripomastigotaAmastigotaCardiomiócitoTrypanosoma cruziTrypomastigoteAmastigoteCardiomyocyteSTAT3 GlycosylphosphatidylinositolTrypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, GermanyDepartment of Cardiology, Philipps University, Marburg, Germany/Department of Cardiology, Philipps University, Marburg, GermanyDepartment of Psychosomatic Medicine and Psychotherapy, Georg August University, 37075 Gottingen, Germany/ Department of Cardiology, Philipps University, Marburg, GermanyLaboratory of Parasitology. Institute for Virology. Philipps University. Marburg, GermanyFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil/Division of Infectious Diseases and Immunology. Department of Medicine. University of Massachusetts Medical School, Worcester, MA, USA/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilDepartment of Cardiology, Philipps University, Marburg, GermanyCNRS-UMR 8576, Unit of Structural and Functional Glycobiology. Univ Lille Nord de France, Lille, France/ Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, GermanyUniversite Francois Rabelais de Tours. UMR 1282 Infectiologie et Sante Publique. Tours, France/INRA, UMR 1282 Infectiologie et Sante Publique. Nouzilly, France/Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, GermanyThe haemoflagellate Trypanosoma cruzi is the causative agent of Chagas' disease that occurs in approximately 8 million people in Latin America. Patients infected with T. cruzi frequently suffer of cardiomegaly and may die of myocardial failure. Here we show that T. cruzi trypomastigotes (extracellular form) increased in vitro apoptosis of rat cardiomyocytes. Additionally, we demonstrated that amastigotes (intracellular form), for which a method for purification was established, were also able to induce cardiomyocyte apoptosis. Increase of apoptosis was associated with up-regulation of the apoptotic gene bax by trypomastigotes, while expression of the anti-apoptotic gene bcl-2 was down-regulated by amastigotes. The transcription factor STAT3 but not STAT1 was activated in cardiomyocytes by trypomastigotes. In addition, tlr7 gene expression was up-regulated in cardiomyocytes incubated with trypomastigotes, suggesting that this Toll-like receptor is involved in the intracellular recognition after host cell invasion by T. cruzi. Glycosylphosphatidylinositols purified from trypomastigotes did not induce cardiomyocyte apoptosis and STAT activation but down-regulated tlr7 gene expression. In conclusion, cardiomyopathy observed in Chagas' disease might be in part due to apoptosis of cardiomyocytes induced directly by the parasite.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30195/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALTrypomastigotes and amastigotes of Trypanosoma .pdfTrypomastigotes and amastigotes of Trypanosoma .pdfapplication/pdf292955https://www.arca.fiocruz.br/bitstream/icict/30195/2/Trypomastigotes%20and%20amastigotes%20of%20Trypanosoma%20.pdf69a42f280e222f0c1cbf009d0ec871a8MD52TEXTTrypomastigotes and amastigotes of Trypanosoma .pdf.txtTrypomastigotes and amastigotes of Trypanosoma .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
title Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
spellingShingle Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
Stahl, Philipp
Trypanosoma cruzi
tripomastigota
Amastigota
Cardiomiócito
Trypanosoma cruzi
Trypomastigote
Amastigote
Cardiomyocyte
STAT3 Glycosylphosphatidylinositol
title_short Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
title_full Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
title_fullStr Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
title_full_unstemmed Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
title_sort Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro.
author Stahl, Philipp
author_facet Stahl, Philipp
Ruppert, Volker
Meyer, Thomas
Schmidt, Jorg
Campos, Marco Antonio
Gazzinelli, Ricardo Tostes
Maisch, Bernhard
Schwarz, Ralph T.
Debierre-Grockiego, Francoise
author_role author
author2 Ruppert, Volker
Meyer, Thomas
Schmidt, Jorg
Campos, Marco Antonio
Gazzinelli, Ricardo Tostes
Maisch, Bernhard
Schwarz, Ralph T.
Debierre-Grockiego, Francoise
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Stahl, Philipp
Ruppert, Volker
Meyer, Thomas
Schmidt, Jorg
Campos, Marco Antonio
Gazzinelli, Ricardo Tostes
Maisch, Bernhard
Schwarz, Ralph T.
Debierre-Grockiego, Francoise
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
tripomastigota
Amastigota
Cardiomiócito
topic Trypanosoma cruzi
tripomastigota
Amastigota
Cardiomiócito
Trypanosoma cruzi
Trypomastigote
Amastigote
Cardiomyocyte
STAT3 Glycosylphosphatidylinositol
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Trypomastigote
Amastigote
Cardiomyocyte
STAT3 Glycosylphosphatidylinositol
description 2020-01-01
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-11-26T16:06:06Z
dc.date.available.fl_str_mv 2018-11-26T16:06:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv STAHL, Philipp et al. Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro. Apoptosis., v. 18, n. 6, p. 653-63, 2013
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30195
dc.identifier.issn.pt_BR.fl_str_mv 1360-8185
dc.identifier.doi.none.fl_str_mv 10.1007/s10495-013-0822-x
identifier_str_mv STAHL, Philipp et al. Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro. Apoptosis., v. 18, n. 6, p. 653-63, 2013
1360-8185
10.1007/s10495-013-0822-x
url https://www.arca.fiocruz.br/handle/icict/30195
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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