Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins

Detalhes bibliográficos
Autor(a) principal: Menezes, Juliana Perrone Bezerra de
Data de Publicação: 2017
Outros Autores: Koushik, Amrita, Das, Satarupa, Guven, Can, Siegel, Ariel, Silva, Maria Fernanda Laranjeira, Losert, Wolfgang, Andrews, Norma W
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18177
Resumo: National Institutes of Health grant R01 AI67979 to NWA. WL and SD were supported by National Institutes of Health grant R01 GM085574. JPBN was supported by a Ciências sem Fronteiras fellowship (CNPq).
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spelling Menezes, Juliana Perrone Bezerra deKoushik, AmritaDas, SatarupaGuven, CanSiegel, ArielSilva, Maria Fernanda LaranjeiraLosert, WolfgangAndrews, Norma W2017-03-27T17:11:00Z2017-03-27T17:11:00Z2017MENEZES, J. P. B. et al. Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins. Cellular Microbiology, v. 19, p. e12668, 2017.1462-5814https://www.arca.fiocruz.br/handle/icict/1817710.1111/cmi.12668National Institutes of Health grant R01 AI67979 to NWA. WL and SD were supported by National Institutes of Health grant R01 GM085574. JPBN was supported by a Ciências sem Fronteiras fellowship (CNPq).University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Physics. Maryland, USAUniversity of Maryland. Department of Physics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Physics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USALeishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-healing skin lesions to fatal visceralizing disease. As the host cells of choice for all species of Leishmania, macrophages are critical for the establishment of infections. How macrophages contribute to parasite homing to specific tissues and how parasites modulate macrophage function are still poorly understood. In this study, we show that Leishmania amazonensis infection inhibits macrophage roaming motility. The reduction in macrophage speed is not dependent on particle load or on factors released by infected macrophages. L. amazonensis-infected macrophages also show reduced directional migration in response to the chemokine MCP-1. We found that infected macrophages have lower levels of total paxillin, phosphorylated paxillin, and phosphorylated focal adhesion kinase when compared to noninfected macrophages, indicating abnormalities in the formation of signaling adhesion complexes that regulate motility. Analysis of the dynamics of actin polymerization at peripheral sites also revealed a markedly enhanced F-actin turnover frequency in L. amazonensis-infected macrophages. Thus, Leishmania infection inhibits macrophage motility by altering actin dynamics and impairing the expression of proteins that function in plasma membrane-extracellular matrix interactions.engWileyLeishmaniaMacrófagosLeishmania amazonensisInfecçãoProteínasPeleLeishmaniaMacrophagesLeishmania amazonensisinfectionProteinsSkinLeishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18177/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMenezes JPB Leishmania infection....pdfMenezes JPB Leishmania 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dc.title.pt_BR.fl_str_mv Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
title Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
spellingShingle Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
Menezes, Juliana Perrone Bezerra de
Leishmania
Macrófagos
Leishmania amazonensis
Infecção
Proteínas
Pele
Leishmania
Macrophages
Leishmania amazonensis
infection
Proteins
Skin
title_short Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
title_full Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
title_fullStr Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
title_full_unstemmed Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
title_sort Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
author Menezes, Juliana Perrone Bezerra de
author_facet Menezes, Juliana Perrone Bezerra de
Koushik, Amrita
Das, Satarupa
Guven, Can
Siegel, Ariel
Silva, Maria Fernanda Laranjeira
Losert, Wolfgang
Andrews, Norma W
author_role author
author2 Koushik, Amrita
Das, Satarupa
Guven, Can
Siegel, Ariel
Silva, Maria Fernanda Laranjeira
Losert, Wolfgang
Andrews, Norma W
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes, Juliana Perrone Bezerra de
Koushik, Amrita
Das, Satarupa
Guven, Can
Siegel, Ariel
Silva, Maria Fernanda Laranjeira
Losert, Wolfgang
Andrews, Norma W
dc.subject.other.pt_BR.fl_str_mv Leishmania
Macrófagos
Leishmania amazonensis
Infecção
Proteínas
Pele
topic Leishmania
Macrófagos
Leishmania amazonensis
Infecção
Proteínas
Pele
Leishmania
Macrophages
Leishmania amazonensis
infection
Proteins
Skin
dc.subject.en.pt_BR.fl_str_mv Leishmania
Macrophages
Leishmania amazonensis
infection
Proteins
Skin
description National Institutes of Health grant R01 AI67979 to NWA. WL and SD were supported by National Institutes of Health grant R01 GM085574. JPBN was supported by a Ciências sem Fronteiras fellowship (CNPq).
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-03-27T17:11:00Z
dc.date.available.fl_str_mv 2017-03-27T17:11:00Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENEZES, J. P. B. et al. Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins. Cellular Microbiology, v. 19, p. e12668, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18177
dc.identifier.issn.pt_BR.fl_str_mv 1462-5814
dc.identifier.doi.none.fl_str_mv 10.1111/cmi.12668
identifier_str_mv MENEZES, J. P. B. et al. Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins. Cellular Microbiology, v. 19, p. e12668, 2017.
1462-5814
10.1111/cmi.12668
url https://www.arca.fiocruz.br/handle/icict/18177
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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