Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major

Detalhes bibliográficos
Autor(a) principal: Menezes, J. P. B.
Data de Publicação: 2013
Outros Autores: Almeida, T. F., Petersen, Antonio Luis de Oliveira Almeida;, Guedes, C. E. S., Mota, M. S. V., Lima, J. G. B., Palma, L. C., Buck, G. A., Krieger, M. A., Probst, C. M., Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15005
Resumo: Texto completo: acesso restrito. p. 579–591
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spelling Menezes, J. P. B.Almeida, T. F.Petersen, Antonio Luis de Oliveira Almeida;Guedes, C. E. S.Mota, M. S. V.Lima, J. G. B.Palma, L. C.Buck, G. A.Krieger, M. A.Probst, C. M.Veras, Patrícia Sampaio TavaresMenezes, J. P. B.Almeida, T. F.Petersen, Antonio Luis de Oliveira Almeida;Guedes, C. E. S.Mota, M. S. V.Lima, J. G. B.Palma, L. C.Buck, G. A.Krieger, M. A.Probst, C. M.Veras, Patrícia Sampaio Tavares2014-05-20T16:44:07Z20131286-4579http://repositorio.ufba.br/ri/handle/ri/15005v. 15, n. 8-9Texto completo: acesso restrito. p. 579–591CBA macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. Employing a transcriptomic approach, we previously showed the up-regulation of the genes involved in the classical pathway of macrophage activation in resistant mice. However, microarray analyses do not evaluate changes in gene expression that occur after translation. To circumvent this analytical limitation, we employed a proteomics approach to increase our understanding of the modulations that occur during infection and identify novel targets for the control of Leishmania infection. To identify proteins whose expression changes in CBA macrophages infected with L. major or L. amazonensis, protein extracts were obtained and digested and the peptides were characterized using multi-dimensional liquid chromatography coupled with tandem mass spectrometry analyses. A total of 162 proteins were selected as potentially modulated. Using biological network analyses, these proteins were classified as primarily involved in cellular metabolism and grouped into cellular development biological networks. This study is the first to use a proteomics approach to describe the protein modulations involved in cellular metabolism during the initial events of Leishmania–macrophage interaction. Based on these findings, we hypothesize that these differentially expressed proteins likely play a pivotal role in determining the course of infection.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-05-20T16:44:07Z No. of bitstreams: 1 1-s2.0-S1286457913000865-main.pdf: 1459187 bytes, checksum: 8d7186a26071a6aedf0690a3c922fa6a (MD5)Made available in DSpace on 2014-05-20T16:44:07Z (GMT). No. of bitstreams: 1 1-s2.0-S1286457913000865-main.pdf: 1459187 bytes, checksum: 8d7186a26071a6aedf0690a3c922fa6a (MD5) Previous issue date: 2013http://dx.doi.org/10.1016/j.micinf.2013.04.005reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania amazonensisLeishmania majorProteomicsMacrophagesProteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania majorMicrobes and Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S1286457913000865-main.pdf1-s2.0-S1286457913000865-main.pdfapplication/pdf1459187https://repositorio.ufba.br/bitstream/ri/15005/1/1-s2.0-S1286457913000865-main.pdf8d7186a26071a6aedf0690a3c922fa6aMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15005/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1-s2.0-S1286457913000865-main.pdf.txt1-s2.0-S1286457913000865-main.pdf.txtExtracted texttext/plain61711https://repositorio.ufba.br/bitstream/ri/15005/3/1-s2.0-S1286457913000865-main.pdf.txt4c196f4fd0d7570f6ec439a9534e84ffMD53ri/150052022-07-05 14:04:16.165oai:repositorio.ufba.br:ri/15005VGVybW8gZGUgTGljZW4/YSwgbj9vIGV4Y2x1c2l2bywgcGFyYSBvIGRlcD9zaXRvIG5vIFJlcG9zaXQ/cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZCQS4KCiBQZWxvIHByb2Nlc3NvIGRlIHN1Ym1pc3M/byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldSByZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIAplc3NlIHRlcm1vIGRlIGxpY2VuP2EsIGNvbmNlZGUgYW8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSAKbyBkaXJlaXRvIGRlIG1hbnRlciB1bWEgYz9waWEgZW0gc2V1IHJlcG9zaXQ/cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2YT8/by4gCkVzc2VzIHRlcm1vcywgbj9vIGV4Y2x1c2l2b3MsIG1hbnQ/bSBvcyBkaXJlaXRvcyBkZSBhdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIApjb21vIHBhcnRlIGRvIGFjZXJ2byBpbnRlbGVjdHVhbCBkZXNzYSBVbml2ZXJzaWRhZGUuCgogUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVpPz9vLCBlc3NlIHRlcm1vIGRlIGxpY2VuP2EgCmVudGVuZGUgcXVlOgoKIE1hbnRlbmRvIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCByZXBhc3NhZG9zIGEgdGVyY2Vpcm9zLCBlbSBjYXNvIGRlIHB1YmxpY2E/P2VzLCBvIHJlcG9zaXQ/cmlvCnBvZGUgcmVzdHJpbmdpciBvIGFjZXNzbyBhbyB0ZXh0byBpbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1hPz9lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHU/P28gY2llbnQ/ZmljYSBjb20gCmFzIHJlc3RyaT8/ZXMgaW1wb3N0YXMgcGVsb3MgZWRpdG9yZXMgZGUgcGVyaT9kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2E/P2VzIHNlbSBpbmljaWF0aXZhcyBxdWUgc2VndWVtIGEgcG9sP3RpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVwP3NpdG9zIApjb21wdWxzP3Jpb3MgbmVzc2UgcmVwb3NpdD9yaW8gbWFudD9tIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudD9tIGFjZXNzbyBpcnJlc3RyaXRvIAphbyBtZXRhZGFkb3MgZSB0ZXh0byBjb21wbGV0by4gQXNzaW0sIGEgYWNlaXRhPz9vIGRlc3NlIHRlcm1vIG4/byBuZWNlc3NpdGEgZGUgY29uc2VudGltZW50bwogcG9yIHBhcnRlIGRlIGF1dG9yZXMvZGV0ZW50b3JlcyBkb3MgZGlyZWl0b3MsIHBvciBlc3RhcmVtIGVtIGluaWNpYXRpdmFzIGRlIGFjZXNzbyBhYmVydG8uCg==Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:16Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
dc.title.alternative.pt_BR.fl_str_mv Microbes and Infection
title Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
spellingShingle Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
Menezes, J. P. B.
Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
title_short Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
title_full Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
title_fullStr Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
title_full_unstemmed Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
title_sort Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major
author Menezes, J. P. B.
author_facet Menezes, J. P. B.
Almeida, T. F.
Petersen, Antonio Luis de Oliveira Almeida;
Guedes, C. E. S.
Mota, M. S. V.
Lima, J. G. B.
Palma, L. C.
Buck, G. A.
Krieger, M. A.
Probst, C. M.
Veras, Patrícia Sampaio Tavares
author_role author
author2 Almeida, T. F.
Petersen, Antonio Luis de Oliveira Almeida;
Guedes, C. E. S.
Mota, M. S. V.
Lima, J. G. B.
Palma, L. C.
Buck, G. A.
Krieger, M. A.
Probst, C. M.
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes, J. P. B.
Almeida, T. F.
Petersen, Antonio Luis de Oliveira Almeida;
Guedes, C. E. S.
Mota, M. S. V.
Lima, J. G. B.
Palma, L. C.
Buck, G. A.
Krieger, M. A.
Probst, C. M.
Veras, Patrícia Sampaio Tavares
Menezes, J. P. B.
Almeida, T. F.
Petersen, Antonio Luis de Oliveira Almeida;
Guedes, C. E. S.
Mota, M. S. V.
Lima, J. G. B.
Palma, L. C.
Buck, G. A.
Krieger, M. A.
Probst, C. M.
Veras, Patrícia Sampaio Tavares
dc.subject.por.fl_str_mv Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
topic Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
description Texto completo: acesso restrito. p. 579–591
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-20T16:44:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15005
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.number.pt_BR.fl_str_mv v. 15, n. 8-9
identifier_str_mv 1286-4579
v. 15, n. 8-9
url http://repositorio.ufba.br/ri/handle/ri/15005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.micinf.2013.04.005
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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