Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18034 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
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Almeida, Tarcísio Vila Verde Santana deFernandes, Jamille SouzaLopes, Diego MotaAndrade, Lorena SantanaOliveira, Sérgio CostaCarvalho Filho, Edgar MarcelinoAraujo, Maria IlmaCruz, Álvaro ACardoso, Luciana Santos2017-03-14T14:18:50Z2017-03-14T14:18:50Z2017ALMEIDA, T. V. V. S. et al. Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma. Acta Tropica, v. 166, p. 268–279, 2017.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/1803410.1016/j.actatropica.2016.12.002Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilInstituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Universidade Federal de Minas Gerias. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / UFBA. ProAR—Núcleo de Excelência em Asma. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / UFBA. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilAsthma is a chronic disease characterized by airway inflammation, obstruction and hyperresponsiveness. Severe asthma affects a small proportion of subjects but results in most of the morbidity, costs and mortality associated with the disease. Studies have suggested that Schistosoma mansoni infection reduces the severity of asthma and prevent atopy. Objective: We evaluated the ability of S. mansoni antigens, Sm29 and Sm29TSP-2 to modulate lymphocyteactivation status in response to the allergen of the mite Dermatophagoides pteronyssinus (Der p1) in cellcultures of individuals with asthma.Methods: Thirty four patients were enrolled in this study: seventeen patients with severe asthma (SAgroup), seventeen patients with mild asthma (MA group) and six controls with no asthma. Peripheralblood mononuclear cells (PBMC) were obtained and stimulated with Sm29 and Sm29TSP-2 in the pres-ence or absence of Der p1. The expression of surface markers and cytokines on lymphocytes was evaluatedby flow cytometry and the levels of IL-10 in the culture supernatant were determined by ELISA.Results: The addition of Sm29 and Sm29TSP-2 antigens to PBMC cultures from both groups of subjectswith asthma stimulated with Der p1 reduced the frequency of CD4+CD25lowcells whereas and increasedfrequency of CD4+CD25highpopulation was observed compared to unstimulated cultures. Moreover, cul-tures stimulated with Sm29TSP-2 showed a reduction in the frequency of T cells expressing CD69, IFN- ,TNF and TGF- in the MA group and an increase in the frequency of CD4+TSLPR+T cells in the SA group.The addition of Sm29 to the cultures reduced the frequency of CD4+CD69+and CD4+IL-5+T cells in all asth-matic groups, and reduced the frequency of CD4+T cells expressing IL-13 in the MA group. The culturesstimulated with Sm29 and Sm29TSP-2 showed an increase in the level of IL-10 in the supernatants.Conclusion: These results suggest that the addition of Sm29 and Sm29TSP-2 to the cells cultures fromsubjects with asthma reduced cell activation markers and altered the cytokine production pattern in away that can potentialy control the inflammatory response associated with asthmaengElsevierAsmaAntígenos de Schistosoma mansoniLinfócitosSm29Sm29TSP-2aAsthmaSchistosoma mansoni antigensLymphocytesSm29Sm29TSP-2aSchistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthmainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18034/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlmeida TVS Scgistosoma mansoni antigens....pdfAlmeida TVS Scgistosoma mansoni antigens....pdfapplication/pdf2217438https://www.arca.fiocruz.br/bitstream/icict/18034/2/Almeida%20TVS%20Scgistosoma%20mansoni%20antigens....pdf7ec6c80f61a253f1cf85fedd25c91066MD52TEXTAlmeida TVS Scgistosoma mansoni antigens....pdf.txtAlmeida TVS Scgistosoma mansoni antigens....pdf.txtExtracted texttext/plain77480https://www.arca.fiocruz.br/bitstream/icict/18034/3/Almeida%20TVS%20Scgistosoma%20mansoni%20antigens....pdf.txt854f03f033b24021d34d8b5c4abb31ebMD53icict/180342023-03-15 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| dc.title.pt_BR.fl_str_mv |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| title |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| spellingShingle |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma Almeida, Tarcísio Vila Verde Santana de Asma Antígenos de Schistosoma mansoni Linfócitos Sm29 Sm29TSP-2a Asthma Schistosoma mansoni antigens Lymphocytes Sm29 Sm29TSP-2a |
| title_short |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| title_full |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| title_fullStr |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| title_full_unstemmed |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| title_sort |
Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma |
| author |
Almeida, Tarcísio Vila Verde Santana de |
| author_facet |
Almeida, Tarcísio Vila Verde Santana de Fernandes, Jamille Souza Lopes, Diego Mota Andrade, Lorena Santana Oliveira, Sérgio Costa Carvalho Filho, Edgar Marcelino Araujo, Maria Ilma Cruz, Álvaro A Cardoso, Luciana Santos |
| author_role |
author |
| author2 |
Fernandes, Jamille Souza Lopes, Diego Mota Andrade, Lorena Santana Oliveira, Sérgio Costa Carvalho Filho, Edgar Marcelino Araujo, Maria Ilma Cruz, Álvaro A Cardoso, Luciana Santos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida, Tarcísio Vila Verde Santana de Fernandes, Jamille Souza Lopes, Diego Mota Andrade, Lorena Santana Oliveira, Sérgio Costa Carvalho Filho, Edgar Marcelino Araujo, Maria Ilma Cruz, Álvaro A Cardoso, Luciana Santos |
| dc.subject.other.pt_BR.fl_str_mv |
Asma Antígenos de Schistosoma mansoni Linfócitos Sm29 Sm29TSP-2a |
| topic |
Asma Antígenos de Schistosoma mansoni Linfócitos Sm29 Sm29TSP-2a Asthma Schistosoma mansoni antigens Lymphocytes Sm29 Sm29TSP-2a |
| dc.subject.en.pt_BR.fl_str_mv |
Asthma Schistosoma mansoni antigens Lymphocytes Sm29 Sm29TSP-2a |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
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2017 |
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2017-03-14T14:18:50Z |
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2017-03-14T14:18:50Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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ALMEIDA, T. V. V. S. et al. Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma. Acta Tropica, v. 166, p. 268–279, 2017. |
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https://www.arca.fiocruz.br/handle/icict/18034 |
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0001-706X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2016.12.002 |
| identifier_str_mv |
ALMEIDA, T. V. V. S. et al. Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma. Acta Tropica, v. 166, p. 268–279, 2017. 0001-706X 10.1016/j.actatropica.2016.12.002 |
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Elsevier |
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Elsevier |
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