Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/14745 |
Resumo: | Texto completo: acesso restrito. p. 538–548 |
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Carvalho Filho, Edgar Marcelino deOliveira, Sergio CostaCardoso, Luciana SantosSouza, Robson da Paixão deGóes, Alfredo Miranda deOliveira, Ricardo RiccioAlcântara, Leda MariaAlmeida, M. C.Araujo, Maria Ilma Andrade SantosCarvalho Filho, Edgar Marcelino deOliveira, Sergio CostaCardoso, Luciana SantosSouza, Robson da Paixão deGóes, Alfredo Miranda deOliveira, Ricardo RiccioAlcântara, Leda MariaAlmeida, M. C.Araujo, Maria Ilma Andrade Santos2014-03-13T18:22:05Z20110272-4391http://repositorio.ufba.br/ri/handle/ri/14745v. 72, n. 6Texto completo: acesso restrito. p. 538–548Schistosoma mansoni infection is associated with a low prevalence of asthma and a less severe form of the disease. The mechanisms underlying this association may include the production of regulatory cells and cytokines. The aim of this study was to evaluate the immune response induced by the S. mansoni antigens, Sm22.6, PIII, and Sm29 and their ability to suppress allergen-specific IL-5 production by peripheral blood mononuclear cells (PBMC) from asthmatic individuals. PBMCs were stimulated in vitro with S. mansoni antigens in the presence or absence of antigen-1 of the mite Dermatophagoides pteronyssinus (Der p1). Cytokines were measured in PBMC supernatants by enzyme-linked immunosorbent assay (ELISA), and the phenotype of cells producing IL-10 was assessed using flow cytometry. High production of S. mansoni antigen-specific IL-10 was observed not only in cells of S. mansoni-infected individuals, but also in cells of noninfected asthmatic individuals. In the former group, the main cellular sources of IL-10 were CD4+ CD25+, and CD14+ cells. The levels of IFN-γ, IL-5, and IL-13 in the noninfected asthmatic group were ∼100 pg/ml in response to the antigens. Moreover, when S. mansoni antigens were added to cultures stimulated with Der p1, levels of IL-10 were increased (Der p1 = 234 ± 118; Der p1 + Sm22.6 = 1189 ± 595; Derp1 + PIII = 799 ± 331; Derp1 + Sm29 = 652 ± 288 pg/ml) with reduced levels of IL-5 (Der p1 = 286 ± 219; Der p1 + Sm22.6 = 93 ± 153; Derp1 + PIII = 132 ± 188; Derp1 + Sm29 = 96 ± 86 pg/ml). The S. mansoni antigens evaluated in the present study induced the production of the regulatory cytokine IL-10 and down-modulated the Th2 immune response that participates in the pathology of asthma. Drug Dev Res 72:538–548, 2011. © 2011 Wiley-Liss, Inc.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-02-25T11:59:18Z No. of bitstreams: 1 E. M. Carvalho.pdf: 158066 bytes, checksum: cfd891827908a4a8e15e9ec760f24234 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:22:05Z (GMT) No. of bitstreams: 1 E. M. Carvalho.pdf: 158066 bytes, checksum: cfd891827908a4a8e15e9ec760f24234 (MD5)Made available in DSpace on 2014-03-13T18:22:05Z (GMT). No. of bitstreams: 1 E. M. Carvalho.pdf: 158066 bytes, checksum: cfd891827908a4a8e15e9ec760f24234 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1002/ddr.20459reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAsthmaSchistosoma mansoni antigensInterleukin-10Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individualsDrug Development Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALE. M. Carvalho.pdfE. M. 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| dc.title.pt_BR.fl_str_mv |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| dc.title.alternative.pt_BR.fl_str_mv |
Drug Development Research |
| title |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| spellingShingle |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals Carvalho Filho, Edgar Marcelino de Asthma Schistosoma mansoni antigens Interleukin-10 |
| title_short |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| title_full |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| title_fullStr |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| title_full_unstemmed |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| title_sort |
Schistosoma mansoni antigens modulate allergic response in vitro in cells of asthmatic individuals |
| author |
Carvalho Filho, Edgar Marcelino de |
| author_facet |
Carvalho Filho, Edgar Marcelino de Oliveira, Sergio Costa Cardoso, Luciana Santos Souza, Robson da Paixão de Góes, Alfredo Miranda de Oliveira, Ricardo Riccio Alcântara, Leda Maria Almeida, M. C. Araujo, Maria Ilma Andrade Santos |
| author_role |
author |
| author2 |
Oliveira, Sergio Costa Cardoso, Luciana Santos Souza, Robson da Paixão de Góes, Alfredo Miranda de Oliveira, Ricardo Riccio Alcântara, Leda Maria Almeida, M. C. Araujo, Maria Ilma Andrade Santos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho Filho, Edgar Marcelino de Oliveira, Sergio Costa Cardoso, Luciana Santos Souza, Robson da Paixão de Góes, Alfredo Miranda de Oliveira, Ricardo Riccio Alcântara, Leda Maria Almeida, M. C. Araujo, Maria Ilma Andrade Santos Carvalho Filho, Edgar Marcelino de Oliveira, Sergio Costa Cardoso, Luciana Santos Souza, Robson da Paixão de Góes, Alfredo Miranda de Oliveira, Ricardo Riccio Alcântara, Leda Maria Almeida, M. C. Araujo, Maria Ilma Andrade Santos |
| dc.subject.por.fl_str_mv |
Asthma Schistosoma mansoni antigens Interleukin-10 |
| topic |
Asthma Schistosoma mansoni antigens Interleukin-10 |
| description |
Texto completo: acesso restrito. p. 538–548 |
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2011 |
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2011 |
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2014-03-13T18:22:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/14745 |
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0272-4391 |
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v. 72, n. 6 |
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0272-4391 v. 72, n. 6 |
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http://repositorio.ufba.br/ri/handle/ri/14745 |
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http://dx.doi.org/ 10.1002/ddr.20459 |
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