Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31364 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparativa e Ambiental. Rio de Janeiro, RJ. Brasil. |
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Melgaço, Fabiana GilCorrêa, Adriana AbreuGanime, Ana CarolinaBrandão, Marcelo Luiz LimaMedeiros, Valéria de MelloRosas, Carla de OliveiraLopes, Silvia Maria dos ReisMiagostovich, Marize Pereira2019-01-31T15:47:34Z2019-01-31T15:47:34Z2018MELGAÇO, Fabiana Gil; et al. Evaluation of skimmed milk flocculation method for virus recovery from tomatoes, Brazilian Journal od Microbiology, v.495, p.34-39, 2018.1517-8382https://www.arca.fiocruz.br/handle/icict/3136410.1016/j.bjm.2018.04.0141678-4405engSociedade Brasileira de MicrobiologiaAdenovirusNorovirusTomatesNorovirus murino 1FloculaçãoAdenovirusFlocculationMurine norovirus-1NorovirusTomatoEvaluation of skimmed milk flocculation method for virus recovery from tomatoesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparativa e Ambiental. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Laboratório de Virologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparativa e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Imunologia. Laboratório de Vacinas Virais, Biofarmacêuticos e Cultura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Setor de Alimentos. Laboratório de Alimentos e Saneantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Setor de Alimentos. Laboratório de Alimentos e Saneantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Setor de Alimentos. Laboratório de Alimentos e Saneantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparativa e Ambiental. Rio de Janeiro, RJ. Brasil.This study aimed to evaluate the elution-concentration methodology based on skimmedmilk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanumlycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further moni-toring of field samples. Spiking experiments were performed to determine the success rateand efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1)used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%,2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differencesaccording to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of90 samples, obtained at commercial establishments in the metropolitan region of Rio deJaneiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed withno detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated thatthe skimmed milk flocculation method is suitable for recovering HAdV from tomatoes andhighlights the need for considering investigation in order to improve food safety.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31364/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfabianagil_melgaço_etal_IOC_2018.pdffabianagil_melgaço_etal_IOC_2018.pdfapplication/pdf855187https://www.arca.fiocruz.br/bitstream/icict/31364/2/fabianagil_melga%c3%a7o_etal_IOC_2018.pdf41fd433ec51e89d6fcd9ca9f4562bb1fMD52TEXTfabianagil_melgaço_etal_IOC_2018.pdf.txtfabianagil_melgaço_etal_IOC_2018.pdf.txtExtracted texttext/plain29088https://www.arca.fiocruz.br/bitstream/icict/31364/3/fabianagil_melga%c3%a7o_etal_IOC_2018.pdf.txt8c837408ff1622e1b43c9e0ed7a69090MD53icict/313642019-02-01 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| dc.title.pt_BR.fl_str_mv |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| title |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| spellingShingle |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes Melgaço, Fabiana Gil Adenovirus Norovirus Tomates Norovirus murino 1 Floculação Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
| title_short |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| title_full |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| title_fullStr |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| title_full_unstemmed |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| title_sort |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
| author |
Melgaço, Fabiana Gil |
| author_facet |
Melgaço, Fabiana Gil Corrêa, Adriana Abreu Ganime, Ana Carolina Brandão, Marcelo Luiz Lima Medeiros, Valéria de Mello Rosas, Carla de Oliveira Lopes, Silvia Maria dos Reis Miagostovich, Marize Pereira |
| author_role |
author |
| author2 |
Corrêa, Adriana Abreu Ganime, Ana Carolina Brandão, Marcelo Luiz Lima Medeiros, Valéria de Mello Rosas, Carla de Oliveira Lopes, Silvia Maria dos Reis Miagostovich, Marize Pereira |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Melgaço, Fabiana Gil Corrêa, Adriana Abreu Ganime, Ana Carolina Brandão, Marcelo Luiz Lima Medeiros, Valéria de Mello Rosas, Carla de Oliveira Lopes, Silvia Maria dos Reis Miagostovich, Marize Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Adenovirus Norovirus Tomates Norovirus murino 1 Floculação |
| topic |
Adenovirus Norovirus Tomates Norovirus murino 1 Floculação Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
| dc.subject.en.pt_BR.fl_str_mv |
Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparativa e Ambiental. Rio de Janeiro, RJ. Brasil. |
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2018 |
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2018 |
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2019-01-31T15:47:34Z |
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2019-01-31T15:47:34Z |
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MELGAÇO, Fabiana Gil; et al. Evaluation of skimmed milk flocculation method for virus recovery from tomatoes, Brazilian Journal od Microbiology, v.495, p.34-39, 2018. |
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https://www.arca.fiocruz.br/handle/icict/31364 |
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1517-8382 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.bjm.2018.04.014 |
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1678-4405 |
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MELGAÇO, Fabiana Gil; et al. Evaluation of skimmed milk flocculation method for virus recovery from tomatoes, Brazilian Journal od Microbiology, v.495, p.34-39, 2018. 1517-8382 10.1016/j.bjm.2018.04.014 1678-4405 |
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Sociedade Brasileira de Microbiologia |
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Sociedade Brasileira de Microbiologia |
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