Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034 |
Resumo: | Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety. |
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Brazilian Journal of Microbiology |
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Evaluation of skimmed milk flocculation method for virus recovery from tomatoesAdenovirusFlocculationMurine norovirus-1NorovirusTomatoAbstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.Sociedade Brasileira de Microbiologia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034Brazilian Journal of Microbiology v.49 suppl.1 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2018.04.014info:eu-repo/semantics/openAccessMelgaço,Fabiana GilCorrêa,Adriana AbreuGanime,Ana CarolinaBrandão,Marcelo Luiz LimaMedeiros,Valéria de MelloRosas,Carla de OliveiraLopes,Silvia Maria dos ReisMiagostovich,Marize Pereiraeng2018-11-29T00:00:00Zoai:scielo:S1517-83822018000500034Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
title |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
spellingShingle |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes Melgaço,Fabiana Gil Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
title_short |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
title_full |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
title_fullStr |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
title_full_unstemmed |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
title_sort |
Evaluation of skimmed milk flocculation method for virus recovery from tomatoes |
author |
Melgaço,Fabiana Gil |
author_facet |
Melgaço,Fabiana Gil Corrêa,Adriana Abreu Ganime,Ana Carolina Brandão,Marcelo Luiz Lima Medeiros,Valéria de Mello Rosas,Carla de Oliveira Lopes,Silvia Maria dos Reis Miagostovich,Marize Pereira |
author_role |
author |
author2 |
Corrêa,Adriana Abreu Ganime,Ana Carolina Brandão,Marcelo Luiz Lima Medeiros,Valéria de Mello Rosas,Carla de Oliveira Lopes,Silvia Maria dos Reis Miagostovich,Marize Pereira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Melgaço,Fabiana Gil Corrêa,Adriana Abreu Ganime,Ana Carolina Brandão,Marcelo Luiz Lima Medeiros,Valéria de Mello Rosas,Carla de Oliveira Lopes,Silvia Maria dos Reis Miagostovich,Marize Pereira |
dc.subject.por.fl_str_mv |
Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
topic |
Adenovirus Flocculation Murine norovirus-1 Norovirus Tomato |
description |
Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2018.04.014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.49 suppl.1 2018 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122209821786112 |