Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

Detalhes bibliográficos
Autor(a) principal: Melgaço,Fabiana Gil
Data de Publicação: 2018
Outros Autores: Corrêa,Adriana Abreu, Ganime,Ana Carolina, Brandão,Marcelo Luiz Lima, Medeiros,Valéria de Mello, Rosas,Carla de Oliveira, Lopes,Silvia Maria dos Reis, Miagostovich,Marize Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034
Resumo: Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.
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spelling Evaluation of skimmed milk flocculation method for virus recovery from tomatoesAdenovirusFlocculationMurine norovirus-1NorovirusTomatoAbstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.Sociedade Brasileira de Microbiologia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034Brazilian Journal of Microbiology v.49 suppl.1 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2018.04.014info:eu-repo/semantics/openAccessMelgaço,Fabiana GilCorrêa,Adriana AbreuGanime,Ana CarolinaBrandão,Marcelo Luiz LimaMedeiros,Valéria de MelloRosas,Carla de OliveiraLopes,Silvia Maria dos ReisMiagostovich,Marize Pereiraeng2018-11-29T00:00:00Zoai:scielo:S1517-83822018000500034Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
title Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
spellingShingle Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
Melgaço,Fabiana Gil
Adenovirus
Flocculation
Murine norovirus-1
Norovirus
Tomato
title_short Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
title_full Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
title_fullStr Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
title_full_unstemmed Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
title_sort Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
author Melgaço,Fabiana Gil
author_facet Melgaço,Fabiana Gil
Corrêa,Adriana Abreu
Ganime,Ana Carolina
Brandão,Marcelo Luiz Lima
Medeiros,Valéria de Mello
Rosas,Carla de Oliveira
Lopes,Silvia Maria dos Reis
Miagostovich,Marize Pereira
author_role author
author2 Corrêa,Adriana Abreu
Ganime,Ana Carolina
Brandão,Marcelo Luiz Lima
Medeiros,Valéria de Mello
Rosas,Carla de Oliveira
Lopes,Silvia Maria dos Reis
Miagostovich,Marize Pereira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melgaço,Fabiana Gil
Corrêa,Adriana Abreu
Ganime,Ana Carolina
Brandão,Marcelo Luiz Lima
Medeiros,Valéria de Mello
Rosas,Carla de Oliveira
Lopes,Silvia Maria dos Reis
Miagostovich,Marize Pereira
dc.subject.por.fl_str_mv Adenovirus
Flocculation
Murine norovirus-1
Norovirus
Tomato
topic Adenovirus
Flocculation
Murine norovirus-1
Norovirus
Tomato
description Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2018.04.014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.49 suppl.1 2018
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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