Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/29776 |
Resumo: | 2020-01-01 |
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Rocha, Eliseu Soares de OliveiraOliveira, Jaquelline Germano deSantos, João Rodrigues dosRodrigues, Gisele Olinto LibânioFigueiredo, Leandra BarcelosPessanha, José Eduardo MarquesProietti, Fernando AugustoFonseca, Flávio Guimarães daBonjardim, Claudio AntonioFerreira, Paulo Cesar PeregrinoKroon, Erna Geessien2018-10-29T14:03:07Z2018-10-29T14:03:07Z2013ROCHA, Eliseu Soares de Oliveira et al. Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. Journal of Virological Methods, v. 187, n. 1, p. 114-120, 20130166-0934https://www.arca.fiocruz.br/handle/icict/2977610.1016/j.jviromet.2012.09.012engElsevierDengue vírussorologiadiagnosticoELISADengue vírusSerologyDiagnosisELISARecombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilPrefeitura Municipal de Belo Horizonte. Secretaria Municipal de Saúde. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Virologia Comparada. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, BrazilDengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/29776/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALRecombinant envelope protein-based enzyme.pdfRecombinant envelope protein-based enzyme.pdfapplication/pdf4865958https://www.arca.fiocruz.br/bitstream/icict/29776/2/Recombinant%20envelope%20protein-based%20enzyme.pdf5aac9fef5952f9d2098eeb8affd95037MD52TEXTRecombinant envelope protein-based enzyme.pdf.txtRecombinant envelope protein-based enzyme.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| title |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| spellingShingle |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection Rocha, Eliseu Soares de Oliveira Dengue vírus sorologia diagnostico ELISA Dengue vírus Serology Diagnosis ELISA |
| title_short |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| title_full |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| title_fullStr |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| title_full_unstemmed |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| title_sort |
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection |
| author |
Rocha, Eliseu Soares de Oliveira |
| author_facet |
Rocha, Eliseu Soares de Oliveira Oliveira, Jaquelline Germano de Santos, João Rodrigues dos Rodrigues, Gisele Olinto Libânio Figueiredo, Leandra Barcelos Pessanha, José Eduardo Marques Proietti, Fernando Augusto Fonseca, Flávio Guimarães da Bonjardim, Claudio Antonio Ferreira, Paulo Cesar Peregrino Kroon, Erna Geessien |
| author_role |
author |
| author2 |
Oliveira, Jaquelline Germano de Santos, João Rodrigues dos Rodrigues, Gisele Olinto Libânio Figueiredo, Leandra Barcelos Pessanha, José Eduardo Marques Proietti, Fernando Augusto Fonseca, Flávio Guimarães da Bonjardim, Claudio Antonio Ferreira, Paulo Cesar Peregrino Kroon, Erna Geessien |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rocha, Eliseu Soares de Oliveira Oliveira, Jaquelline Germano de Santos, João Rodrigues dos Rodrigues, Gisele Olinto Libânio Figueiredo, Leandra Barcelos Pessanha, José Eduardo Marques Proietti, Fernando Augusto Fonseca, Flávio Guimarães da Bonjardim, Claudio Antonio Ferreira, Paulo Cesar Peregrino Kroon, Erna Geessien |
| dc.subject.other.pt_BR.fl_str_mv |
Dengue vírus sorologia diagnostico ELISA |
| topic |
Dengue vírus sorologia diagnostico ELISA Dengue vírus Serology Diagnosis ELISA |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue vírus Serology Diagnosis ELISA |
| description |
2020-01-01 |
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2013 |
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2013 |
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2018-10-29T14:03:07Z |
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2018-10-29T14:03:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ROCHA, Eliseu Soares de Oliveira et al. Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. Journal of Virological Methods, v. 187, n. 1, p. 114-120, 2013 |
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https://www.arca.fiocruz.br/handle/icict/29776 |
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0166-0934 |
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10.1016/j.jviromet.2012.09.012 |
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ROCHA, Eliseu Soares de Oliveira et al. Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. Journal of Virological Methods, v. 187, n. 1, p. 114-120, 2013 0166-0934 10.1016/j.jviromet.2012.09.012 |
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