Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45538 |
Resumo: | 1 Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, Brazil, 2 D’Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil, 3 Gonc¸alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil, 4 Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil, 5 Department of Pharmacy, Federal University of Paraíba, João Pessoa, Brazil |
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Cavalcante, Bruno R. R.França, Luciana S. AragãoSampaio, Gabriela L. A.Nonaka, Carolina K. V.Oliveira, Moisés S.Campos, Gúbio S.Sardi, Silvia I.Dias, Beatriz Rocha SimõesMenezes, Juliana Perrone Bezerra de.Rocha, Vinícius P. C.Rossi, Erik A.Paredes, Bruno D.Martins, Gabriele L. S.Allahdadi, Kyan J.Peixoto, Laisla R.Barbosa Filho, José M.Souza, Bruno S. F.Soares, Milena Botelho Pereira2021-01-08T11:20:03Z2021-01-08T11:20:03Z2020CAVALCANTE, Bruno R. R. et al. Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells. Frontiers in Cellular and Infection Microbiology, p. 1-13, 2020.2235-2988https://www.arca.fiocruz.br/handle/icict/4553810.3389/fcimb.2020.5583241 Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, Brazil, 2 D’Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil, 3 Gonc¸alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil, 4 Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil, 5 Department of Pharmacy, Federal University of Paraíba, João Pessoa, BrazilBrazilian National Council for Scientific and Technological Development (CNPq), DECIT/MS (grant number 443909/2018-0), INCT-REGENERA (grant number 465656/2014‐5) and the Coordination for the Improvement of Higher Education Personnel (CAPES/Zika Fast track).Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil.Universidade Federal da Paraíba. Centro de Ciências da Saúde. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.Universidade Federal da Paraíba. Centro de Ciências da Saúde. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto D'Or de Ensino e Pesquisa. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Zika virus (ZIKV), a member of the Flaviviridae family, was brought into the spotlight due to its widespread and increased pathogenicity, including Guillain-Barré syndrome and microcephaly. Neural progenitor cells (NPCs), which are multipotent cells capable of differentiating into the major neural phenotypes, are very susceptible to ZIKV infection. Given the complications of ZIKV infection and potential harm to public health, effective treatment options are urgently needed. Betulinic acid (BA), an abundant terpenoid of the lupane group, displays several biological activities, including neuroprotective effects. Here we demonstrate that Sox2+ NPCs, which are highly susceptible to ZIKV when compared to their neuronal counterparts, are protected against ZIKV-induced cell death when treated with BA. Similarly, the population of Sox2+ and Casp3+ NPCs found in ZIKVinfected cerebral organoids was significantly higher in the presence of BA than in untreated controls. Moreover, well-preserved structures were found in BA-treated organoids in contrast to ZIKV-infected controls. Bioinformatics analysis indicated Akt pathway activation by BA treatment. This was confirmed by phosphorylated Akt analysis, both in BA-treated NPCs and brain organoids, as shown by immunoblotting and immunofluorescence analyses, respectively. Taken together, these data suggest a neuroprotective role of BA in ZIKV-infected NPCs.engFrontiers MediaZika vírusCélulas progenitorasÁcido betulínicoApoptoseNeuroproteçãoZika virusNeural progenitor cellsBetulinic acidApoptosisNeuroprotectionBetulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45538/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCavalcante Bruno RR Betulinic Acid....pdfCavalcante Bruno RR Betulinic Acid....pdfapplication/pdf2278090https://www.arca.fiocruz.br/bitstream/icict/45538/2/Cavalcante%20Bruno%20RR%20Betulinic%20Acid....pdf087c30b1a530fd5890dd0ddbc53b497aMD52TEXTCavalcante Bruno RR Betulinic Acid....pdf.txtCavalcante Bruno RR Betulinic Acid....pdf.txtExtracted texttext/plain58719https://www.arca.fiocruz.br/bitstream/icict/45538/3/Cavalcante%20Bruno%20RR%20Betulinic%20Acid....pdf.txtcd30a9cbbb86268772661d322eee5dc6MD53icict/455382023-03-15 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| dc.title.pt_BR.fl_str_mv |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| title |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| spellingShingle |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells Cavalcante, Bruno R. R. Zika vírus Células progenitoras Ácido betulínico Apoptose Neuroproteção Zika virus Neural progenitor cells Betulinic acid Apoptosis Neuroprotection |
| title_short |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| title_full |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| title_fullStr |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| title_full_unstemmed |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| title_sort |
Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells |
| author |
Cavalcante, Bruno R. R. |
| author_facet |
Cavalcante, Bruno R. R. França, Luciana S. Aragão Sampaio, Gabriela L. A. Nonaka, Carolina K. V. Oliveira, Moisés S. Campos, Gúbio S. Sardi, Silvia I. Dias, Beatriz Rocha Simões Menezes, Juliana Perrone Bezerra de. Rocha, Vinícius P. C. Rossi, Erik A. Paredes, Bruno D. Martins, Gabriele L. S. Allahdadi, Kyan J. Peixoto, Laisla R. Barbosa Filho, José M. Souza, Bruno S. F. Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
França, Luciana S. Aragão Sampaio, Gabriela L. A. Nonaka, Carolina K. V. Oliveira, Moisés S. Campos, Gúbio S. Sardi, Silvia I. Dias, Beatriz Rocha Simões Menezes, Juliana Perrone Bezerra de. Rocha, Vinícius P. C. Rossi, Erik A. Paredes, Bruno D. Martins, Gabriele L. S. Allahdadi, Kyan J. Peixoto, Laisla R. Barbosa Filho, José M. Souza, Bruno S. F. Soares, Milena Botelho Pereira |
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author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cavalcante, Bruno R. R. França, Luciana S. Aragão Sampaio, Gabriela L. A. Nonaka, Carolina K. V. Oliveira, Moisés S. Campos, Gúbio S. Sardi, Silvia I. Dias, Beatriz Rocha Simões Menezes, Juliana Perrone Bezerra de. Rocha, Vinícius P. C. Rossi, Erik A. Paredes, Bruno D. Martins, Gabriele L. S. Allahdadi, Kyan J. Peixoto, Laisla R. Barbosa Filho, José M. Souza, Bruno S. F. Soares, Milena Botelho Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Zika vírus Células progenitoras Ácido betulínico Apoptose Neuroproteção |
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Zika vírus Células progenitoras Ácido betulínico Apoptose Neuroproteção Zika virus Neural progenitor cells Betulinic acid Apoptosis Neuroprotection |
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Zika virus Neural progenitor cells Betulinic acid Apoptosis Neuroprotection |
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1 Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, Brazil, 2 D’Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil, 3 Gonc¸alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil, 4 Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil, 5 Department of Pharmacy, Federal University of Paraíba, João Pessoa, Brazil |
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2020 |
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2021-01-08T11:20:03Z |
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2021-01-08T11:20:03Z |
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CAVALCANTE, Bruno R. R. et al. Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells. Frontiers in Cellular and Infection Microbiology, p. 1-13, 2020. |
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10.3389/fcimb.2020.558324 |
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CAVALCANTE, Bruno R. R. et al. Betulinic Acid Exerts Cytoprotective Activity on Zika Virus-Infected Neural Progenitor Cells. Frontiers in Cellular and Infection Microbiology, p. 1-13, 2020. 2235-2988 10.3389/fcimb.2020.558324 |
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