Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51851 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
| id |
CRUZ_a44530e09c0c2a6cf0592b060ce84eb8 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/51851 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Falavinha, Bruna CristinaBarisón, María JuliaRebelatto, Carmen Lúcia KuniyoshiMarcon, Bruna HilzendegerAguiar, Alessandra de MeloSilva, Evelin Brandão daStimamiglio, Marco AugustoShigunov, Patrícia2022-03-24T12:26:41Z2022-03-24T12:26:41Z2022FALAVINHA, Bruna Cristina et al. Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells. Stem Cells International, p. 1–14, 2022.1687-9678https://www.arca.fiocruz.br/handle/icict/5185110.1155/2022/4930932porHindawiInterleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Escola de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Dysfunctions in adipose tissue cells are responsible for several obesity-related metabolic diseases. Understanding the process of adipocyte formation is thus fundamental for understanding these diseases. The adipocyte differentiation of adipose-derived stem/stromal cells (ADSCs) showed a reduction in the mRNA level of the interleukin 21 receptor (IL21R) during this process. Although the receptor has been associated with metabolic diseases, few studies have examined its function in stem cells. In this study, we used confocal immunofluorescence assays to determine that IL21R colocalizes with mitochondrial protein ATP5B, ALDH4A1, and the nucleus of human ADSCs. We demonstrated that silencing and overexpression of IL21R did not affect the cell proliferation and mitochondrial activity of ADSCs. However, IL21R silencing did reduce ADSC adipogenic capacity. Further studies are needed to understand the mechanism involved between IL21R and the adipogenic differentiation process.AdipocytesMesenchymal Stem CellsReceptors, Interleukin-21AdipocitosCélulas Madre MesenquimatosasReceptores de Interleucina-21AdipocytesCellules souches mésenchymateusesRécepteurs à l'interleukine-21AdipócitosCélulas-Tronco MesenquimaisReceptores de Interleucina-21info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/51851/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSCI2022-4930932OK.pdfSCI2022-4930932OK.pdfapplication/pdf5412500https://www.arca.fiocruz.br/bitstream/icict/51851/2/SCI2022-4930932OK.pdf73d170e7471687fb92f5b4c9aeaaf526MD52TEXTSCI2022-4930932OK.pdf.txtSCI2022-4930932OK.pdf.txtExtracted texttext/plain52232https://www.arca.fiocruz.br/bitstream/icict/51851/3/SCI2022-4930932OK.pdf.txt8525dff47f7de47d024a626a8f7a4897MD53icict/518512022-03-25 02:00:10.809oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-25T05:00:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| title |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| spellingShingle |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells Falavinha, Bruna Cristina Adipocytes Mesenchymal Stem Cells Receptors, Interleukin-21 Adipocitos Células Madre Mesenquimatosas Receptores de Interleucina-21 Adipocytes Cellules souches mésenchymateuses Récepteurs à l'interleukine-21 Adipócitos Células-Tronco Mesenquimais Receptores de Interleucina-21 |
| title_short |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| title_full |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| title_fullStr |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| title_full_unstemmed |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| title_sort |
Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells |
| author |
Falavinha, Bruna Cristina |
| author_facet |
Falavinha, Bruna Cristina Barisón, María Julia Rebelatto, Carmen Lúcia Kuniyoshi Marcon, Bruna Hilzendeger Aguiar, Alessandra de Melo Silva, Evelin Brandão da Stimamiglio, Marco Augusto Shigunov, Patrícia |
| author_role |
author |
| author2 |
Barisón, María Julia Rebelatto, Carmen Lúcia Kuniyoshi Marcon, Bruna Hilzendeger Aguiar, Alessandra de Melo Silva, Evelin Brandão da Stimamiglio, Marco Augusto Shigunov, Patrícia |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Falavinha, Bruna Cristina Barisón, María Julia Rebelatto, Carmen Lúcia Kuniyoshi Marcon, Bruna Hilzendeger Aguiar, Alessandra de Melo Silva, Evelin Brandão da Stimamiglio, Marco Augusto Shigunov, Patrícia |
| dc.subject.en.pt_BR.fl_str_mv |
Adipocytes Mesenchymal Stem Cells Receptors, Interleukin-21 |
| topic |
Adipocytes Mesenchymal Stem Cells Receptors, Interleukin-21 Adipocitos Células Madre Mesenquimatosas Receptores de Interleucina-21 Adipocytes Cellules souches mésenchymateuses Récepteurs à l'interleukine-21 Adipócitos Células-Tronco Mesenquimais Receptores de Interleucina-21 |
| dc.subject.es.pt_BR.fl_str_mv |
Adipocitos Células Madre Mesenquimatosas Receptores de Interleucina-21 |
| dc.subject.fr.pt_BR.fl_str_mv |
Adipocytes Cellules souches mésenchymateuses Récepteurs à l'interleukine-21 |
| dc.subject.decs.pt_BR.fl_str_mv |
Adipócitos Células-Tronco Mesenquimais Receptores de Interleucina-21 |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
| publishDate |
2022 |
| dc.date.accessioned.fl_str_mv |
2022-03-24T12:26:41Z |
| dc.date.available.fl_str_mv |
2022-03-24T12:26:41Z |
| dc.date.issued.fl_str_mv |
2022 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
FALAVINHA, Bruna Cristina et al. Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells. Stem Cells International, p. 1–14, 2022. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/51851 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1687-9678 |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2022/4930932 |
| identifier_str_mv |
FALAVINHA, Bruna Cristina et al. Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells. Stem Cells International, p. 1–14, 2022. 1687-9678 10.1155/2022/4930932 |
| url |
https://www.arca.fiocruz.br/handle/icict/51851 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Hindawi |
| publisher.none.fl_str_mv |
Hindawi |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/51851/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/51851/2/SCI2022-4930932OK.pdf https://www.arca.fiocruz.br/bitstream/icict/51851/3/SCI2022-4930932OK.pdf.txt |
| bitstream.checksum.fl_str_mv |
783568c2893d2e25a99990b126be1772 73d170e7471687fb92f5b4c9aeaaf526 8525dff47f7de47d024a626a8f7a4897 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324692020363264 |