Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes

Detalhes bibliográficos
Autor(a) principal: Spangenberg, Lucia
Data de Publicação: 2013
Outros Autores: Shigunov, Patrícia, Abud, Ana Paula Ressetti, Cofré, Axel Helmut Rulf, Stimamiglio, Marco Augusto, Kuligovski, Crisciele, Zych, Jaiesa, Schittini, Andressa V., Costa, Alexandre Dias Tavares, Rebelatto, Carmen Lúcia Kuniyoshi, Brofman, Paulo Roberto Slud, Goldenberg, Samuel, Correa, Alejandro, Naya, Hugo, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20681
Resumo: Institut Pasteur Montevideo. Unidad de Bioinformática. Montevideo, Uruguay.
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spelling Spangenberg, LuciaShigunov, PatríciaAbud, Ana Paula RessettiCofré, Axel Helmut RulfStimamiglio, Marco AugustoKuligovski, CriscieleZych, JaiesaSchittini, Andressa V.Costa, Alexandre Dias TavaresRebelatto, Carmen Lúcia KuniyoshiBrofman, Paulo Roberto SludGoldenberg, SamuelCorrea, AlejandroNaya, HugoDallagiovanna, Bruno2017-08-18T14:28:45Z2017-08-18T14:28:45Z2013SPANGENBERG, Lucia et al. Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes. Stem Cell Research, n. 11, p. 902-912, 2013.1876-7753https://www.arca.fiocruz.br/handle/icict/2068110.1016/j.scr.2013.06.002porElsevierDiferenciação CelularCell DifferentiationAdipocytesStem CellsDiferenciación CelularAdipocitosCélulas MadreAdipócitosCélulas-TroncoPolysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur Montevideo. Unidad de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Institut Pasteur Montevideo. Unidad de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Adipocyte stem cells (hASCs) can proliferate and self-renew and, due to their multipotent nature, they can differentiate into several tissue-specific lineages, making them ideal candidates for use in cell therapy. Most attempts to determine the mRNA profile of self-renewing or differentiating stem cells have made use of total RNA for gene expression analysis. Several lines of evidence suggest that self-renewal and differentiation are also dependent on the control of protein synthesis by posttranscriptional mechanisms. We used adipogenic differentiation as a model, to investigate the extent to which posttranscriptional regulation controlled gene expression in hASCs. We focused on the initial steps of differentiation and isolated both the total mRNA fraction and the subpopulation of mRNAs associated with translating ribosomes. We observed that adipogenesis is committed in the first days of induction and three days appears as the minimum time of induction necessary for efficient differentiation. RNA-seq analysis showed that a significant percentage of regulated mRNAs were posttranscriptionally controlled. Part of this regulation involves massive changes in transcript untranslated regions (UTR) length, with differential extension/reduction of the 3'UTR after induction. A slight correlation can be observed between the expression levels of differentially expressed genes and the 3'UTR length. When we considered association to polysomes, this correlation values increased. Changes in the half lives were related to the extension of the 3'UTR, with longer UTRs mainly stabilizing the transcripts. Thus, changes in the length of these extensions may be associated with changes in the ability to associate with polysomes or in half-life.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20681/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSpangenbergpoly.pdfSpangenbergpoly.pdfapplication/pdf1003715https://www.arca.fiocruz.br/bitstream/icict/20681/2/Spangenbergpoly.pdf4e042c381ca6191b3ccac780cc2def23MD52TEXTSpangenbergpoly.pdf.txtSpangenbergpoly.pdf.txtExtracted texttext/plain57766https://www.arca.fiocruz.br/bitstream/icict/20681/3/Spangenbergpoly.pdf.txt695274bb8575abf3609d030e916413bcMD53icict/206812021-12-23 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dc.title.pt_BR.fl_str_mv Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
title Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
spellingShingle Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
Spangenberg, Lucia
Diferenciação Celular
Cell Differentiation
Adipocytes
Stem Cells
Diferenciación Celular
Adipocitos
Células Madre
Adipócitos
Células-Tronco
title_short Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
title_full Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
title_fullStr Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
title_full_unstemmed Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
title_sort Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
author Spangenberg, Lucia
author_facet Spangenberg, Lucia
Shigunov, Patrícia
Abud, Ana Paula Ressetti
Cofré, Axel Helmut Rulf
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Zych, Jaiesa
Schittini, Andressa V.
Costa, Alexandre Dias Tavares
Rebelatto, Carmen Lúcia Kuniyoshi
Brofman, Paulo Roberto Slud
Goldenberg, Samuel
Correa, Alejandro
Naya, Hugo
Dallagiovanna, Bruno
author_role author
author2 Shigunov, Patrícia
Abud, Ana Paula Ressetti
Cofré, Axel Helmut Rulf
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Zych, Jaiesa
Schittini, Andressa V.
Costa, Alexandre Dias Tavares
Rebelatto, Carmen Lúcia Kuniyoshi
Brofman, Paulo Roberto Slud
Goldenberg, Samuel
Correa, Alejandro
Naya, Hugo
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Spangenberg, Lucia
Shigunov, Patrícia
Abud, Ana Paula Ressetti
Cofré, Axel Helmut Rulf
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Zych, Jaiesa
Schittini, Andressa V.
Costa, Alexandre Dias Tavares
Rebelatto, Carmen Lúcia Kuniyoshi
Brofman, Paulo Roberto Slud
Goldenberg, Samuel
Correa, Alejandro
Naya, Hugo
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Diferenciação Celular
topic Diferenciação Celular
Cell Differentiation
Adipocytes
Stem Cells
Diferenciación Celular
Adipocitos
Células Madre
Adipócitos
Células-Tronco
dc.subject.en.pt_BR.fl_str_mv Cell Differentiation
Adipocytes
Stem Cells
dc.subject.es.pt_BR.fl_str_mv Diferenciación Celular
Adipocitos
Células Madre
dc.subject.decs.pt_BR.fl_str_mv Adipócitos
Células-Tronco
description Institut Pasteur Montevideo. Unidad de Bioinformática. Montevideo, Uruguay.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-08-18T14:28:45Z
dc.date.available.fl_str_mv 2017-08-18T14:28:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SPANGENBERG, Lucia et al. Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes. Stem Cell Research, n. 11, p. 902-912, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20681
dc.identifier.issn.pt_BR.fl_str_mv 1876-7753
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.scr.2013.06.002
identifier_str_mv SPANGENBERG, Lucia et al. Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes. Stem Cell Research, n. 11, p. 902-912, 2013.
1876-7753
10.1016/j.scr.2013.06.002
url https://www.arca.fiocruz.br/handle/icict/20681
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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