Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34244 |
Resumo: | Inclui Retraction: Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens, February 16, 2022. |
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Silva, Aurea Virginia Andrade daFigueiredo, Fabiano BorgesMenezes, Rodrigo CaldasMendes-Junior, Arthur AugustoMiranda, Luisa Helena Monteiro deCupolillo, ElisaPorrozzi, RenatoMorgado, Fernanda Nazaré2019-07-18T14:31:06Z2019-07-18T14:31:06Z2018SILVA, Aurea Virginia Andrade da et al. Morphophysiological changes in the splenic extracellular matrix of Leishmania infantumnaturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens. Plos Neglected Tropical Diseases, p. 1-16, Apr. 2018.1935-2727https://www.arca.fiocruz.br/handle/icict/3424410.1371/journal.pntd.00064451935-2735Inclui Retraction: Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens, February 16, 2022.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmaniose. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-graduação Stricto Sensu em Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmaniose. Rio de Janeiro, RJ, Brasil.The spleen is one of the main affected organs in canine visceral leishmaniasis (CVL). Disorganization of the splenic white pulp (SWP) has been associated with immunosuppression and disease progression. This study aims to assess structural and cellular changes in the splenic extracellular matrix of dogs with CVL, correlating these changes with the parasite load and clinical signs. Splenic fragments were collected from 41 naturally infected animals for parasite load quantification by quantitative PCR, histopathological analysis and immunohistochemistry for CD3+, CD4+, and CD8+ T cells; CD21+ B cells; Ki-67+, IFN-γ+, and IL-10+ cells; and the MMP-9 and ADAM-10 enzymes. Laminin, collagen and fibronectin deposition were also evaluated. The animals were grouped according to the level of SWP organization. SWP disorganization was accompanied by a reduction in the quantity of lymphoid follicles/mm2 (p > 0.0001). Animals with moderate to intense SWP disorganization showed more clinical signs (p = 0.021), higher laminin (p = 0.045) and collagen deposition (p = 0.036), higher MMP-9 expression (p = 0.035) and lower numbers of CD4+ T cells (p = 0.027) in the spleen than the animals with organized SWP. These data suggest that splenic structure and function are drastically altered and compromised during CVL.engPublic Library of ScienceMorphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania infantumDogsSpleeninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| title |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| spellingShingle |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens Silva, Aurea Virginia Andrade da Leishmania infantum Dogs Spleen |
| title_short |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| title_full |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| title_fullStr |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| title_full_unstemmed |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| title_sort |
Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens |
| author |
Silva, Aurea Virginia Andrade da |
| author_facet |
Silva, Aurea Virginia Andrade da Figueiredo, Fabiano Borges Menezes, Rodrigo Caldas Mendes-Junior, Arthur Augusto Miranda, Luisa Helena Monteiro de Cupolillo, Elisa Porrozzi, Renato Morgado, Fernanda Nazaré |
| author_role |
author |
| author2 |
Figueiredo, Fabiano Borges Menezes, Rodrigo Caldas Mendes-Junior, Arthur Augusto Miranda, Luisa Helena Monteiro de Cupolillo, Elisa Porrozzi, Renato Morgado, Fernanda Nazaré |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Aurea Virginia Andrade da Figueiredo, Fabiano Borges Menezes, Rodrigo Caldas Mendes-Junior, Arthur Augusto Miranda, Luisa Helena Monteiro de Cupolillo, Elisa Porrozzi, Renato Morgado, Fernanda Nazaré |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania infantum Dogs Spleen |
| topic |
Leishmania infantum Dogs Spleen |
| description |
Inclui Retraction: Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens, February 16, 2022. |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018 |
| dc.date.accessioned.fl_str_mv |
2019-07-18T14:31:06Z |
| dc.date.available.fl_str_mv |
2019-07-18T14:31:06Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SILVA, Aurea Virginia Andrade da et al. Morphophysiological changes in the splenic extracellular matrix of Leishmania infantumnaturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens. Plos Neglected Tropical Diseases, p. 1-16, Apr. 2018. |
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https://www.arca.fiocruz.br/handle/icict/34244 |
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1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0006445 |
| dc.identifier.eissn.none.fl_str_mv |
1935-2735 |
| identifier_str_mv |
SILVA, Aurea Virginia Andrade da et al. Morphophysiological changes in the splenic extracellular matrix of Leishmania infantumnaturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens. Plos Neglected Tropical Diseases, p. 1-16, Apr. 2018. 1935-2727 10.1371/journal.pntd.0006445 1935-2735 |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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