Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46290 |
Resumo: | Program for Technological Development of Tools for Health (PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology in Health and Investigative Medicine. |
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Santos, Catiule de OliveiraCosta, Sidnei FerroSouza, Fabiana SantanaMendes, Jessica Mariane FerreiraPinheiro, Cristiane Garboggini Melo deMoreira, Diogo Rodrigo de MagalhãesSilva, Luciano KalabricLima, Valeria Marçal Felix deOliveira, Geraldo Gileno de Sá2021-03-08T12:26:01Z2021-03-08T12:26:01Z2021SANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021.1932-6203https://www.arca.fiocruz.br/handle/icict/4629010.1371/journal.pone.0239171Program for Technological Development of Tools for Health (PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology in Health and Investigative Medicine.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Bahia, Brasil.São Paulo State University. School of Veterinary Medicine. Department of Clinical Medicine, Surgery and Animal Reproduction. Araçatuba, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil.São Paulo State University. School of Veterinary Medicine. Department of Clinical Medicine, Surgery and Animal Reproduction. Araçatuba, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil.rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.engPublic Library of ScienceLeishmanioseCãesInterleucina-10Leishmania infantumTécnicas In VitroLeishmaniasisDogsInterleukin-10Leishmania infantumIn Vitro TechniquesBlocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/46290/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALSantos, Oliveira Catiule, Blocking IL....pdfSantos, Oliveira Catiule, Blocking IL....pdfapplication/pdf1082565https://www.arca.fiocruz.br/bitstream/icict/46290/2/Santos%2c%20Oliveira%20Catiule%2c%20Blocking%20IL....pdfc855b5de224848bba899f9491f134bfeMD52TEXTSantos, Oliveira Catiule, Blocking IL....pdf.txtSantos, Oliveira Catiule, Blocking IL....pdf.txtExtracted texttext/plain59919https://www.arca.fiocruz.br/bitstream/icict/46290/3/Santos%2c%20Oliveira%20Catiule%2c%20Blocking%20IL....pdf.txtf72e3290bde50a05625bd11131d5f92eMD53icict/462902023-03-15 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| dc.title.pt_BR.fl_str_mv |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| title |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| spellingShingle |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum Santos, Catiule de Oliveira Leishmaniose Cães Interleucina-10 Leishmania infantum Técnicas In Vitro Leishmaniasis Dogs Interleukin-10 Leishmania infantum In Vitro Techniques |
| title_short |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| title_full |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| title_fullStr |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| title_full_unstemmed |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| title_sort |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
| author |
Santos, Catiule de Oliveira |
| author_facet |
Santos, Catiule de Oliveira Costa, Sidnei Ferro Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de Oliveira, Geraldo Gileno de Sá |
| author_role |
author |
| author2 |
Costa, Sidnei Ferro Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de Oliveira, Geraldo Gileno de Sá |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Catiule de Oliveira Costa, Sidnei Ferro Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de Oliveira, Geraldo Gileno de Sá |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Cães Interleucina-10 Leishmania infantum Técnicas In Vitro |
| topic |
Leishmaniose Cães Interleucina-10 Leishmania infantum Técnicas In Vitro Leishmaniasis Dogs Interleukin-10 Leishmania infantum In Vitro Techniques |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Dogs Interleukin-10 Leishmania infantum In Vitro Techniques |
| description |
Program for Technological Development of Tools for Health (PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology in Health and Investigative Medicine. |
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2021 |
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2021-03-08T12:26:01Z |
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2021-03-08T12:26:01Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46290 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0239171 |
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SANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021. 1932-6203 10.1371/journal.pone.0239171 |
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