Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids

Detalhes bibliográficos
Autor(a) principal: Cortes, S
Data de Publicação: 2018
Outros Autores: Wendt, A Albuquerque, Maia, C, Carvalho, M, Lima, I A, Freitas, Luiz Antonio Rodrigues de, dosSantos, Washington Luis Conrado, Campino, L
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30651
Resumo: Fundação para a Ciência e a Tecnologia (FCT), Ministério da Ciência, Tecnologia e Ensino Superior to the project PTDC/CVT/112371/2009 and GHTM (UID/Multi/04413/2013) and Rede CYTED (RIMLEV Propuesta P210RT0656). S. Cortes and C. Maia have the support of FCT, through the Investigator Starting Grants IF/0773/2015 and IF/01302/2015, respectively.
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spelling Cortes, SWendt, A AlbuquerqueMaia, CCarvalho, MLima, I AFreitas, Luiz Antonio Rodrigues dedosSantos, Washington Luis ConradoCampino, L2018-12-18T12:33:03Z2018-12-18T12:33:03Z2018CORTES, L. et al. Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids. Parasitology, 2018.0031-1820https://www.arca.fiocruz.br/handle/icict/3065110.1017/S0031182018001993Fundação para a Ciência e a Tecnologia (FCT), Ministério da Ciência, Tecnologia e Ensino Superior to the project PTDC/CVT/112371/2009 and GHTM (UID/Multi/04413/2013) and Rede CYTED (RIMLEV Propuesta P210RT0656). S. Cortes and C. Maia have the support of FCT, through the Investigator Starting Grants IF/0773/2015 and IF/01302/2015, respectively.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Global Health and Tropical Medicine. Lisbon, Portugal.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Global Health and Tropical Medicine. Lisbon, Portugal.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Global Health and Tropical Medicine. Lisbon, Portugal.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Global Health and Tropical Medicine. Lisbon, Portugal / Clinilab. Anatomia Patológica. Grupo Galenus. Lisbon, Portugal.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Global Health and Tropical Medicine. Lisbon, Portugal.The clinical manifestation and course of Leishmania infections depend on factors such as species, virulence and host-immunity. Although trypanosomatids are considered to have clonal propagation, genetic hybridization has produced successful natural hybrid lineages. Hybrids displaying strong selective advantages may have an impact on pathogenesis and the eco-epidemiology of leishmaniasis. Thus, characterization of phenotypic properties of Leishmania hybrids could bring significant insight into the biology, infectivity, pathogenicity and transmission dynamics of these atypical strains. The present study focuses on phenotypic features and survival capacity of Leishmania infantum/Leishmania major hybrid isolates as compared with representative putative parental species, L. infantum and L. major. In vitro assays (growth kinetics, susceptibility to different conditions) and in vivo infection (parasite detection and histopathological alterations) showed that hybrids present higher growth capacity and decreased susceptibility to reactive oxygen species. Furthermore, evaluation of infected spleen tissue suggests that hybrids induce a stronger immune reaction than their putative parents, leading to the development of white pulp hyperplasia in B-lymphocyte compartments. Overall, these hybrids have shown high plasticity in terms of their general behaviour within the different phenotypic parameters, suggesting that they might have acquired genetic features conferring different mechanisms to evade host cells.engCambridge University PressComportamento in vitroComportamento in vivoHíbridos de LeishmaniaL. infantum / L. principais híbridosFenótipoIn vitro behaviourIn vivo behaviourLeishmania hybridsL. infantum/L. major hybridsPhenotypeElucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybridsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30651/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCortes S. Elucidating in vitro...2018.pdfCortes S. 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dc.title.pt_BR.fl_str_mv Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
title Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
spellingShingle Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
Cortes, S
Comportamento in vitro
Comportamento in vivo
Híbridos de Leishmania
L. infantum / L. principais híbridos
Fenótipo
In vitro behaviour
In vivo behaviour
Leishmania hybrids
L. infantum/L. major hybrids
Phenotype
title_short Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
title_full Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
title_fullStr Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
title_full_unstemmed Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
title_sort Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids
author Cortes, S
author_facet Cortes, S
Wendt, A Albuquerque
Maia, C
Carvalho, M
Lima, I A
Freitas, Luiz Antonio Rodrigues de
dosSantos, Washington Luis Conrado
Campino, L
author_role author
author2 Wendt, A Albuquerque
Maia, C
Carvalho, M
Lima, I A
Freitas, Luiz Antonio Rodrigues de
dosSantos, Washington Luis Conrado
Campino, L
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cortes, S
Wendt, A Albuquerque
Maia, C
Carvalho, M
Lima, I A
Freitas, Luiz Antonio Rodrigues de
dosSantos, Washington Luis Conrado
Campino, L
dc.subject.other.pt_BR.fl_str_mv Comportamento in vitro
Comportamento in vivo
Híbridos de Leishmania
L. infantum / L. principais híbridos
Fenótipo
topic Comportamento in vitro
Comportamento in vivo
Híbridos de Leishmania
L. infantum / L. principais híbridos
Fenótipo
In vitro behaviour
In vivo behaviour
Leishmania hybrids
L. infantum/L. major hybrids
Phenotype
dc.subject.en.pt_BR.fl_str_mv In vitro behaviour
In vivo behaviour
Leishmania hybrids
L. infantum/L. major hybrids
Phenotype
description Fundação para a Ciência e a Tecnologia (FCT), Ministério da Ciência, Tecnologia e Ensino Superior to the project PTDC/CVT/112371/2009 and GHTM (UID/Multi/04413/2013) and Rede CYTED (RIMLEV Propuesta P210RT0656). S. Cortes and C. Maia have the support of FCT, through the Investigator Starting Grants IF/0773/2015 and IF/01302/2015, respectively.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-18T12:33:03Z
dc.date.available.fl_str_mv 2018-12-18T12:33:03Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CORTES, L. et al. Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids. Parasitology, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30651
dc.identifier.issn.pt_BR.fl_str_mv 0031-1820
dc.identifier.doi.none.fl_str_mv 10.1017/S0031182018001993
identifier_str_mv CORTES, L. et al. Elucidating in vitro and in vivo phenotypic behaviour of L. infantum/L. major natural hybrids. Parasitology, 2018.
0031-1820
10.1017/S0031182018001993
url https://www.arca.fiocruz.br/handle/icict/30651
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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