Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles

Detalhes bibliográficos
Autor(a) principal: Costa, Jonas Henrique
Data de Publicação: 2021
Outros Autores: Bazioli, Jaqueline Moraes, Barbosa, Luidy Darllan, Santos Júnior, Pedro Luis Theodoro dos, Reis, Flavia Coelho Garcia dos, Klimeck, Tabata, Crnkovic, Camila Manoel, Berlinck, Roberto G. S., Sussulini, Alessandra, Rodrigues, Marcio Lourenço, Fill, Taícia Pacheco
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46363
https://doi.org/10.1128/mBio.03393-20
Resumo: Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.
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spelling Costa, Jonas HenriqueBazioli, Jaqueline MoraesBarbosa, Luidy DarllanSantos Júnior, Pedro Luis Theodoro dosReis, Flavia Coelho Garcia dosKlimeck, TabataCrnkovic, Camila ManoelBerlinck, Roberto G. S.Sussulini, AlessandraRodrigues, Marcio LourençoFill, Taícia Pacheco2021-03-15T16:48:54Z2021-03-15T16:48:54Z2021COSTA, Jonas Henrique et al. Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles. mBio, v.12, n.1, p. 1-16, 2021.2150-7511https://www.arca.fiocruz.br/handle/icict/46363https://doi.org/10.1128/mBio.03393-20porAmerican Society for MicrobiologyPenicillium digitatumTryptoquialanineFungiExtracellular VesiclesHerbicidesHost-Pathogen InteractionsHongosVesículas ExtracelularesInteracciones Huésped-PatógenoChampignonsVésicules extracellulairesInteractions hôte-pathogèneFungosVesículas ExtracelularesHerbicidasInterações Hospedeiro-PatógenoPenicilliumPhytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesiclesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Campinas. Instituto de Química. Campinas, SP, Brasil.Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. / Universidade de Campinas. Faculdade de Ciências Farmacêuticas. Campinas, SP, Brasil.Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Tecnologia Bioquímico-Farmacêutica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química de São Carlos. São Paulo, SP, Brasil.Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.Penicillium digitatum is the most aggressive pathogen of citrus fruits. Tryptoquialanines are major indole alkaloids produced by P. digitatum. It is unknown if tryptoquialanines are involved in the damage of citrus fruits caused by P. digitatum. To investigate the pathogenic roles of tryptoquialanines, we initially asked if Tryptoquialanines could affect the germination of Citrus sinensis seeds. Exposure of the citrus seeds to tryptoquialanine A resulted in a complete inhibition of germination and an altered metabolic response. Since this phytotoxic effect requires the extracellular export of tryptoquialanine A, we investigated the mechanisms of extracellular delivery of this alkaloid in P. digitatum. We detected extracellular vesicles (EVs) released by P. digitatum both in culture and during infection of citrus fruits. Compositional analysis of EVs produced during infection revealed the presence of a complex cargo, which included tryptoquialanines and the mycotoxin fungisporin. The EVs also presented phytotoxicity activity in vitro and caused damage to the tissues of citrus seeds. Through molecular networking, it was observed that the metabolites present in the P. digitatum EVs are produced in all of its possible hosts. Our results reveal a novel phytopathogenic role of P. digitatum EVs and tryptoquialanine A, implying that this alkaloid is exported in EVs during plant infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/46363/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmBio-2021-Costa-e03393-ok.pdfmBio-2021-Costa-e03393-ok.pdfapplication/pdf3506989https://www.arca.fiocruz.br/bitstream/icict/46363/2/mBio-2021-Costa-e03393-ok.pdf1dc18dbd216ff2c17607bd78ddc77e38MD52TEXTmBio-2021-Costa-e03393-ok.pdf.txtmBio-2021-Costa-e03393-ok.pdf.txtExtracted texttext/plain62994https://www.arca.fiocruz.br/bitstream/icict/46363/3/mBio-2021-Costa-e03393-ok.pdf.txt9b2e3a151df54901dfeecd07295abc94MD53icict/463632022-01-12 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dc.title.pt_BR.fl_str_mv Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
title Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
spellingShingle Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
Costa, Jonas Henrique
Penicillium digitatum
Tryptoquialanine
Fungi
Extracellular Vesicles
Herbicides
Host-Pathogen Interactions
Hongos
Vesículas Extracelulares
Interacciones Huésped-Patógeno
Champignons
Vésicules extracellulaires
Interactions hôte-pathogène
Fungos
Vesículas Extracelulares
Herbicidas
Interações Hospedeiro-Patógeno
Penicillium
title_short Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
title_full Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
title_fullStr Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
title_full_unstemmed Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
title_sort Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles
author Costa, Jonas Henrique
author_facet Costa, Jonas Henrique
Bazioli, Jaqueline Moraes
Barbosa, Luidy Darllan
Santos Júnior, Pedro Luis Theodoro dos
Reis, Flavia Coelho Garcia dos
Klimeck, Tabata
Crnkovic, Camila Manoel
Berlinck, Roberto G. S.
Sussulini, Alessandra
Rodrigues, Marcio Lourenço
Fill, Taícia Pacheco
author_role author
author2 Bazioli, Jaqueline Moraes
Barbosa, Luidy Darllan
Santos Júnior, Pedro Luis Theodoro dos
Reis, Flavia Coelho Garcia dos
Klimeck, Tabata
Crnkovic, Camila Manoel
Berlinck, Roberto G. S.
Sussulini, Alessandra
Rodrigues, Marcio Lourenço
Fill, Taícia Pacheco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Jonas Henrique
Bazioli, Jaqueline Moraes
Barbosa, Luidy Darllan
Santos Júnior, Pedro Luis Theodoro dos
Reis, Flavia Coelho Garcia dos
Klimeck, Tabata
Crnkovic, Camila Manoel
Berlinck, Roberto G. S.
Sussulini, Alessandra
Rodrigues, Marcio Lourenço
Fill, Taícia Pacheco
dc.subject.other.pt_BR.fl_str_mv Penicillium digitatum
Tryptoquialanine
topic Penicillium digitatum
Tryptoquialanine
Fungi
Extracellular Vesicles
Herbicides
Host-Pathogen Interactions
Hongos
Vesículas Extracelulares
Interacciones Huésped-Patógeno
Champignons
Vésicules extracellulaires
Interactions hôte-pathogène
Fungos
Vesículas Extracelulares
Herbicidas
Interações Hospedeiro-Patógeno
Penicillium
dc.subject.en.pt_BR.fl_str_mv Fungi
Extracellular Vesicles
Herbicides
Host-Pathogen Interactions
dc.subject.es.pt_BR.fl_str_mv Hongos
Vesículas Extracelulares
Interacciones Huésped-Patógeno
dc.subject.fr.pt_BR.fl_str_mv Champignons
Vésicules extracellulaires
Interactions hôte-pathogène
dc.subject.decs.pt_BR.fl_str_mv Fungos
Vesículas Extracelulares
Herbicidas
Interações Hospedeiro-Patógeno
Penicillium
description Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-03-15T16:48:54Z
dc.date.available.fl_str_mv 2021-03-15T16:48:54Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Jonas Henrique et al. Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles. mBio, v.12, n.1, p. 1-16, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46363
dc.identifier.issn.pt_BR.fl_str_mv 2150-7511
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/mBio.03393-20
identifier_str_mv COSTA, Jonas Henrique et al. Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles. mBio, v.12, n.1, p. 1-16, 2021.
2150-7511
url https://www.arca.fiocruz.br/handle/icict/46363
https://doi.org/10.1128/mBio.03393-20
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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