The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis

Detalhes bibliográficos
Autor(a) principal: Silva, Everton Fagonde da
Data de Publicação: 2007
Outros Autores: Medeiros, Marco Alberto, McBride, Alan John Alexander, Matsunaga, Jim, Esteves, Gabriela dos Santos, Ramos, João Gabriel Rosa, Santos, Cleiton Silva, Croda, Júlio Henrique Rosa, Homma, Akira, Dellagostin, Odir Antonio, Haake, David A., Reis, Mitermayer Galvão dos, Ko, Albert Icksang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23765
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
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spelling Silva, Everton Fagonde daMedeiros, Marco AlbertoMcBride, Alan John AlexanderMatsunaga, JimEsteves, Gabriela dos SantosRamos, João Gabriel RosaSantos, Cleiton SilvaCroda, Júlio Henrique RosaHomma, AkiraDellagostin, Odir AntonioHaake, David A.Reis, Mitermayer Galvão dosKo, Albert Icksang2017-12-22T16:52:56Z2017-12-22T16:52:56Z2007SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/2376510.1016/j.vaccine.2007.05.053engElsevierhttps://www.arca.fiocruz.br/handle/icict/5818LeptospiroseVacinaProteína recombinanteImunidadeAnticorposHamstersLeptospirosisSubunit vaccineLeptospiral immunoglobulin-like proteinRecombinant proteinImmunityAntibodiesHamstersThe terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. NY, USA.Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23765/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSilva EF The terminal portion....pdfSilva EF The terminal portion....pdfapplication/pdf424129https://www.arca.fiocruz.br/bitstream/icict/23765/2/Silva%20EF%20The%20terminal%20portion....pdf7ad58b6cf7caca6e9128dbbff1f6c196MD52TEXTSilva EF The terminal portion....pdf.txtSilva EF The terminal portion....pdf.txtExtracted texttext/plain51350https://www.arca.fiocruz.br/bitstream/icict/23765/3/Silva%20EF%20The%20terminal%20portion....pdf.txtca78d48e7005a07989a6daaa59054163MD53icict/237652023-03-15 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dc.title.pt_BR.fl_str_mv The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
title The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
spellingShingle The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
Silva, Everton Fagonde da
Leptospirose
Vacina
Proteína recombinante
Imunidade
Anticorpos
Hamsters
Leptospirosis
Subunit vaccine
Leptospiral immunoglobulin-like protein
Recombinant protein
Immunity
Antibodies
Hamsters
title_short The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
title_full The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
title_fullStr The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
title_full_unstemmed The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
title_sort The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
author Silva, Everton Fagonde da
author_facet Silva, Everton Fagonde da
Medeiros, Marco Alberto
McBride, Alan John Alexander
Matsunaga, Jim
Esteves, Gabriela dos Santos
Ramos, João Gabriel Rosa
Santos, Cleiton Silva
Croda, Júlio Henrique Rosa
Homma, Akira
Dellagostin, Odir Antonio
Haake, David A.
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
author_role author
author2 Medeiros, Marco Alberto
McBride, Alan John Alexander
Matsunaga, Jim
Esteves, Gabriela dos Santos
Ramos, João Gabriel Rosa
Santos, Cleiton Silva
Croda, Júlio Henrique Rosa
Homma, Akira
Dellagostin, Odir Antonio
Haake, David A.
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Everton Fagonde da
Medeiros, Marco Alberto
McBride, Alan John Alexander
Matsunaga, Jim
Esteves, Gabriela dos Santos
Ramos, João Gabriel Rosa
Santos, Cleiton Silva
Croda, Júlio Henrique Rosa
Homma, Akira
Dellagostin, Odir Antonio
Haake, David A.
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
dc.subject.other.pt_BR.fl_str_mv Leptospirose
Vacina
Proteína recombinante
Imunidade
Anticorpos
Hamsters
topic Leptospirose
Vacina
Proteína recombinante
Imunidade
Anticorpos
Hamsters
Leptospirosis
Subunit vaccine
Leptospiral immunoglobulin-like protein
Recombinant protein
Immunity
Antibodies
Hamsters
dc.subject.en.pt_BR.fl_str_mv Leptospirosis
Subunit vaccine
Leptospiral immunoglobulin-like protein
Recombinant protein
Immunity
Antibodies
Hamsters
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2017-12-22T16:52:56Z
dc.date.available.fl_str_mv 2017-12-22T16:52:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23765
dc.identifier.issn.pt_BR.fl_str_mv 0264-410X
dc.identifier.doi.none.fl_str_mv 10.1016/j.vaccine.2007.05.053
identifier_str_mv SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007.
0264-410X
10.1016/j.vaccine.2007.05.053
url https://www.arca.fiocruz.br/handle/icict/23765
dc.language.iso.fl_str_mv eng
language eng
dc.relation.isversionof.pt_BR.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5818
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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