The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)

Silva, Éverton Fagonde; Medeiros, Marco Alberto; McBride, Alan John Alexander; Matsunaga, Jim; Esteves, Gabriela S.; Ramos, João G. R; Santos, Cleiton Silva; Croda, Julio Henrique Rosa; Homma, Akira; Dellagostin, Odir Antonio; Haake, David A.; Reis, Mitermayer Galvão dos; Ko, Albert Icksang

The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)

Detalhes bibliográficos
Autor(a) principal:	Silva, Éverton Fagonde
Data de Publicação:	2007
Outros Autores:	Medeiros, Marco Alberto, McBride, Alan John Alexander, Matsunaga, Jim, Esteves, Gabriela S., Ramos, João G. R, Santos, Cleiton Silva, Croda, Julio Henrique Rosa, Homma, Akira, Dellagostin, Odir Antonio, Haake, David A., Reis, Mitermayer Galvão dos, Ko, Albert Icksang
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/5818
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.

Metadados do item

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repository_id_str	2135
spelling	Silva, Éverton FagondeMedeiros, Marco AlbertoMcBride, Alan John AlexanderMatsunaga, JimEsteves, Gabriela S.Ramos, João G. RSantos, Cleiton SilvaCroda, Julio Henrique RosaHomma, AkiraDellagostin, Odir AntonioHaake, David A.Reis, Mitermayer Galvão dosKo, Albert Icksang2012-11-15T02:21:11Z2012-11-15T02:21:11Z2007SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, n. 33, p. 6277-6286, Aug. 2007.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/5818enghttps://www.arca.fiocruz.br/handle/icict/23765The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RGS, Brasil.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis.LeptospirosisSubunit vaccineLeptospiral immunoglobulin-like proteinRecombinant proteinImmunityAntibodiesHamstersProteínas de Bactérias/ImunologiaVacinas BacterianasModelos Animais de DoençasLeptospira/ImunologiaMesocricetus/imunologiaAnimaisNãoNão Publicadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva Everton.pdfSilva Everton.pdfapplication/pdf745194https://www.arca.fiocruz.br/bitstream/icict/5818/1/Silva%20Everton.pdf6f189173f7d221a3ffb0273d9674affdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5818/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTSilva Everton.pdf.txtSilva Everton.pdf.txtExtracted texttext/plain56733https://www.arca.fiocruz.br/bitstream/icict/5818/5/Silva%20Everton.pdf.txt88a9fa22ad06b70471b8495f05589d03MD55THUMBNAILSilva Everton.pdf.jpgSilva Everton.pdf.jpgGenerated Thumbnailimage/jpeg2393https://www.arca.fiocruz.br/bitstream/icict/5818/4/Silva%20Everton.pdf.jpg8736ca765aa0a6017b108f977dfaf610MD54icict/58182021-07-30 16:27:30.893oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-07-30T19:27:30Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
title	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
spellingShingle	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint) Silva, Éverton Fagonde Leptospirosis Subunit vaccine Leptospiral immunoglobulin-like protein Recombinant protein Immunity Antibodies Hamsters Proteínas de Bactérias/Imunologia Vacinas Bacterianas Modelos Animais de Doenças Leptospira/Imunologia Mesocricetus/imunologia Animais
title_short	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
title_full	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
title_fullStr	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
title_full_unstemmed	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
title_sort	The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)
author	Silva, Éverton Fagonde
author_facet	Silva, Éverton Fagonde Medeiros, Marco Alberto McBride, Alan John Alexander Matsunaga, Jim Esteves, Gabriela S. Ramos, João G. R Santos, Cleiton Silva Croda, Julio Henrique Rosa Homma, Akira Dellagostin, Odir Antonio Haake, David A. Reis, Mitermayer Galvão dos Ko, Albert Icksang
author_role	author
author2	Medeiros, Marco Alberto McBride, Alan John Alexander Matsunaga, Jim Esteves, Gabriela S. Ramos, João G. R Santos, Cleiton Silva Croda, Julio Henrique Rosa Homma, Akira Dellagostin, Odir Antonio Haake, David A. Reis, Mitermayer Galvão dos Ko, Albert Icksang
author2_role	author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Silva, Éverton Fagonde Medeiros, Marco Alberto McBride, Alan John Alexander Matsunaga, Jim Esteves, Gabriela S. Ramos, João G. R Santos, Cleiton Silva Croda, Julio Henrique Rosa Homma, Akira Dellagostin, Odir Antonio Haake, David A. Reis, Mitermayer Galvão dos Ko, Albert Icksang
dc.subject.en.pt_BR.fl_str_mv	Leptospirosis Subunit vaccine Leptospiral immunoglobulin-like protein Recombinant protein Immunity Antibodies Hamsters
topic	Leptospirosis Subunit vaccine Leptospiral immunoglobulin-like protein Recombinant protein Immunity Antibodies Hamsters Proteínas de Bactérias/Imunologia Vacinas Bacterianas Modelos Animais de Doenças Leptospira/Imunologia Mesocricetus/imunologia Animais
dc.subject.decs.pt_BR.fl_str_mv	Proteínas de Bactérias/Imunologia Vacinas Bacterianas Modelos Animais de Doenças Leptospira/Imunologia Mesocricetus/imunologia Animais
description	Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate	2007
dc.date.issued.fl_str_mv	2007
dc.date.accessioned.fl_str_mv	2012-11-15T02:21:11Z
dc.date.available.fl_str_mv	2012-11-15T02:21:11Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, n. 33, p. 6277-6286, Aug. 2007.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/5818
dc.identifier.issn.none.fl_str_mv	0264-410X
identifier_str_mv	SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, n. 33, p. 6277-6286, Aug. 2007. 0264-410X
url	https://www.arca.fiocruz.br/handle/icict/5818
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.hasversion.pt_BR.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/23765
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis (preprint)

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