Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1186/1465-9921-11-51 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8295 |
Resumo: | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, Brasil |
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Baqueiro, TianaRusso, MomtchiloSilva, Virgínia Maria Goes daMeirelles, ThaynaOliveira, Pablo Rafael SilveiraGomes, ElianeBarboza, RenatoLima, Ana Tereza CerqueiraFigueiredo, Camila Alexandrina VianaPontes-de-Carvalho, Lain CarlosNeves, Neuza Maria Alcântara2014-08-29T13:55:52Z2014-08-29T13:55:52Z2010BAQUEIRO, T. et al. Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. Respiratory Research, v. 11, p.51, 2010.1465-993Xhttps://www.arca.fiocruz.br/handle/icict/829510.1186/1465-9921-11-51porBioMed CentralRespiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, BrasilUniversidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, BrasilUniversidade de São Paulo. São Paulo. Instituto de Ciencias Biomedicas. Departamento de Imunologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, BrasilBACKGROUND: The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol. OBJECTIVES: This work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses. METHODS: Groups of mice of four different syngeneic strains were sensitized subcutaneously with 100 microg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 microg of BtE. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. A/J mice were also sensitized with a lower dose of BtE. RESULTS: Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Finally, immunization with a relatively low BtE dose (10 microg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses. CONCLUSIONS: The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE.Alérgenos/administração & dosagemAsma/imunologiaPyroglyphidae/imunologiaAdministração IntranasalAnimaisAntígenos de PlantasAsma/genéticaHiper-Reatividade Brônquica/imunologiaLíquido da Lavagem Broncoalveolar/citologiaCitocinas/metabolismoModelos Animais de DoençasRelação Dose-Resposta ImunológicaImunoglobulina E/sangueInjeções SubcutâneasMasculinoCamundongosCamundongos Endogâmicos C57BLCamundongos Endogâmicos BALB COvalbuminaEosinofilia Pulmonar/imunologiaRatosRatos WistarEspecificidade da EspécieFatores de Tempoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8295/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALBaqueiro Tiana Respiratory allergy to Blomia.....pdfBaqueiro Tiana Respiratory allergy to Blomia.....pdfapplication/pdf802715https://www.arca.fiocruz.br/bitstream/icict/8295/2/Baqueiro%20Tiana%20%20Respiratory%20allergy%20to%20Blomia.....pdf749b3089d3dd3840eeab6fa5f5ce3496MD52TEXTBaqueiro Tiana Respiratory allergy to Blomia.....pdf.txtBaqueiro Tiana Respiratory allergy to Blomia.....pdf.txtExtracted texttext/plain44293https://www.arca.fiocruz.br/bitstream/icict/8295/3/Baqueiro%20Tiana%20%20Respiratory%20allergy%20to%20Blomia.....pdf.txtdda76dfadd849c4367530efeb68eb6e4MD53icict/82952023-03-15 14:33:04.913oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| title |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| spellingShingle |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. Baqueiro, Tiana Alérgenos/administração & dosagem Asma/imunologia Pyroglyphidae/imunologia Administração Intranasal Animais Antígenos de Plantas Asma/genética Hiper-Reatividade Brônquica/imunologia Líquido da Lavagem Broncoalveolar/citologia Citocinas/metabolismo Modelos Animais de Doenças Relação Dose-Resposta Imunológica Imunoglobulina E/sangue Injeções Subcutâneas Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos BALB C Ovalbumina Eosinofilia Pulmonar/imunologia Ratos Ratos Wistar Especificidade da Espécie Fatores de Tempo |
| title_short |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| title_full |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| title_fullStr |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| title_full_unstemmed |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| title_sort |
Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. |
| author |
Baqueiro, Tiana |
| author_facet |
Baqueiro, Tiana Russo, Momtchilo Silva, Virgínia Maria Goes da Meirelles, Thayna Oliveira, Pablo Rafael Silveira Gomes, Eliane Barboza, Renato Lima, Ana Tereza Cerqueira Figueiredo, Camila Alexandrina Viana Pontes-de-Carvalho, Lain Carlos Neves, Neuza Maria Alcântara |
| author_role |
author |
| author2 |
Russo, Momtchilo Silva, Virgínia Maria Goes da Meirelles, Thayna Oliveira, Pablo Rafael Silveira Gomes, Eliane Barboza, Renato Lima, Ana Tereza Cerqueira Figueiredo, Camila Alexandrina Viana Pontes-de-Carvalho, Lain Carlos Neves, Neuza Maria Alcântara |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Baqueiro, Tiana Russo, Momtchilo Silva, Virgínia Maria Goes da Meirelles, Thayna Oliveira, Pablo Rafael Silveira Gomes, Eliane Barboza, Renato Lima, Ana Tereza Cerqueira Figueiredo, Camila Alexandrina Viana Pontes-de-Carvalho, Lain Carlos Neves, Neuza Maria Alcântara |
| dc.subject.decs.pt_BR.fl_str_mv |
Alérgenos/administração & dosagem Asma/imunologia Pyroglyphidae/imunologia Administração Intranasal Animais Antígenos de Plantas Asma/genética Hiper-Reatividade Brônquica/imunologia Líquido da Lavagem Broncoalveolar/citologia Citocinas/metabolismo Modelos Animais de Doenças Relação Dose-Resposta Imunológica Imunoglobulina E/sangue Injeções Subcutâneas Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos BALB C Ovalbumina Eosinofilia Pulmonar/imunologia Ratos Ratos Wistar Especificidade da Espécie Fatores de Tempo |
| topic |
Alérgenos/administração & dosagem Asma/imunologia Pyroglyphidae/imunologia Administração Intranasal Animais Antígenos de Plantas Asma/genética Hiper-Reatividade Brônquica/imunologia Líquido da Lavagem Broncoalveolar/citologia Citocinas/metabolismo Modelos Animais de Doenças Relação Dose-Resposta Imunológica Imunoglobulina E/sangue Injeções Subcutâneas Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos BALB C Ovalbumina Eosinofilia Pulmonar/imunologia Ratos Ratos Wistar Especificidade da Espécie Fatores de Tempo |
| description |
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil / Universidade Federal da Bahia. Nucleo de Tecnologia em Saude. Instituto Multidisciplinar em Saude. Vitoria da Conquista, BA, Brasil |
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2010 |
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2010 |
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2014-08-29T13:55:52Z |
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2014-08-29T13:55:52Z |
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info:eu-repo/semantics/article |
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BAQUEIRO, T. et al. Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. Respiratory Research, v. 11, p.51, 2010. |
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https://www.arca.fiocruz.br/handle/icict/8295 |
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1465-993X |
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10.1186/1465-9921-11-51 |
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BAQUEIRO, T. et al. Respiratory allergy to Blomia tropicalis: immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. Respiratory Research, v. 11, p.51, 2010. 1465-993X 10.1186/1465-9921-11-51 |
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BioMed Central |
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BioMed Central |
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