Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells

Detalhes bibliográficos
Autor(a) principal: Araujo, Glauber de S.
Data de Publicação: 2012
Outros Autores: Fonseca, Fernanda L., Pontes, Bruno, Torres, Andre, Cordero, Radames J. B., Zancopé-Oliveira, Rosely M., Casadevall, Arturo, Viana, Nathan B., Nimrichter, Leonardo, Rodrigues, Marcio L., Garcia, Eloi S., Souza, Wanderley de, Frases, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29890
Resumo: Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.
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spelling Araujo, Glauber de S.Fonseca, Fernanda L.Pontes, BrunoTorres, AndreCordero, Radames J. B.Zancopé-Oliveira, Rosely M.Casadevall, ArturoViana, Nathan B.Nimrichter, LeonardoRodrigues, Marcio L.Garcia, Eloi S.Souza, Wanderley deFrases, Susana2018-11-06T13:17:29Z2018-11-06T13:17:29Z2012ARAUJO, Glauber de S. et al. Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells. Plos One, v. 7, n. 1, e29561, p. 1-11, Jan. 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/2989010.1371/journal.pone.0029561engBioMed CentralCryptococcus patogénicoCryptococcus não patogênicoCélulas FagocíticasProteçãoPhagocytic CellsCryptococcus spp.ProtectionPathogenic Cryptococcus spp.Non-Pathogenic Cryptococcus spp.Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. LPO-COPEA. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ. Brasil.Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ. Brasil.Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. LPO-COPEA. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Física. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Capsule production is common among bacterial species, but relatively rare in eukaryotic microorganisms. Members of the fungal Cryptococcus genus are known to produce capsules, which are major determinants of virulence in the highly pathogenic species Cryptococcus neoformans and Cryptococcus gattii. Although the lack of virulence of many species of the Cryptococcus genus can be explained solely by the lack of mammalian thermotolerance, it is uncertain whether the capsules from these organisms are comparable to those of the pathogenic cryptococci. In this study, we compared the characteristic of the capsule from the non-pathogenic environmental yeast Cryptococcus liquefaciens with that of C. neoformans. Microscopic observations revealed that C. liquefaciens has a capsule visible in India ink preparations that was also efficiently labeled by three antibodies generated to specific C. neoformans capsular antigens. Capsular polysaccharides of C. liquefaciens were incorporated onto the cell surface of acapsular C. neoformans mutant cells. Polysaccharide composition determinations in combination with confocal microscopy revealed that C. liquefaciens capsule consisted of mannose, xylose, glucose, glucuronic acid, galactose and N-acetylglucosamine. Physical chemical analysis of the C. liquefaciens polysaccharides in comparison with C. neoformans samples revealed significant differences in viscosity, elastic properties and macromolecular structure parameters of polysaccharide solutions such as rigidity, effective diameter, zeta potential and molecular mass, which nevertheless appeared to be characteristics of linear polysaccharides that also comprise capsular polysaccharide of C. neoformans. The environmental yeast, however, showed enhanced susceptibility to the antimicrobial activity of the environmental phagocytes, suggesting that the C. liquefaciens capsular components are insufficient in protecting yeast cells against killing by amoeba. These results suggest that capsular structures in pathogenic Cryptococcus species and environmental species share similar features, but also manifest significant difference that could influence their potential to virulence.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29890/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALeloiS_garcia_etal_IOC_2012.pdfeloiS_garcia_etal_IOC_2012.pdfapplication/pdf1572444https://www.arca.fiocruz.br/bitstream/icict/29890/2/eloiS_garcia_etal_IOC_2012.pdf70c7fd3535d64524869d8286f0d2f82dMD52TEXTeloiS_garcia_etal_IOC_2012.pdf.txteloiS_garcia_etal_IOC_2012.pdf.txtExtracted texttext/plain56090https://www.arca.fiocruz.br/bitstream/icict/29890/3/eloiS_garcia_etal_IOC_2012.pdf.txt711bd515346f621b9225aba0f661191bMD53icict/298902023-03-02 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dc.title.pt_BR.fl_str_mv Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
title Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
spellingShingle Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
Araujo, Glauber de S.
Cryptococcus patogénico
Cryptococcus não patogênico
Células Fagocíticas
Proteção
Phagocytic Cells
Cryptococcus spp.
Protection
Pathogenic Cryptococcus spp.
Non-Pathogenic Cryptococcus spp.
title_short Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
title_full Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
title_fullStr Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
title_full_unstemmed Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
title_sort Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells
author Araujo, Glauber de S.
author_facet Araujo, Glauber de S.
Fonseca, Fernanda L.
Pontes, Bruno
Torres, Andre
Cordero, Radames J. B.
Zancopé-Oliveira, Rosely M.
Casadevall, Arturo
Viana, Nathan B.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Garcia, Eloi S.
Souza, Wanderley de
Frases, Susana
author_role author
author2 Fonseca, Fernanda L.
Pontes, Bruno
Torres, Andre
Cordero, Radames J. B.
Zancopé-Oliveira, Rosely M.
Casadevall, Arturo
Viana, Nathan B.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Garcia, Eloi S.
Souza, Wanderley de
Frases, Susana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Glauber de S.
Fonseca, Fernanda L.
Pontes, Bruno
Torres, Andre
Cordero, Radames J. B.
Zancopé-Oliveira, Rosely M.
Casadevall, Arturo
Viana, Nathan B.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Garcia, Eloi S.
Souza, Wanderley de
Frases, Susana
dc.subject.other.pt_BR.fl_str_mv Cryptococcus patogénico
Cryptococcus não patogênico
Células Fagocíticas
Proteção
topic Cryptococcus patogénico
Cryptococcus não patogênico
Células Fagocíticas
Proteção
Phagocytic Cells
Cryptococcus spp.
Protection
Pathogenic Cryptococcus spp.
Non-Pathogenic Cryptococcus spp.
dc.subject.en.pt_BR.fl_str_mv Phagocytic Cells
Cryptococcus spp.
Protection
Pathogenic Cryptococcus spp.
Non-Pathogenic Cryptococcus spp.
description Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biotecnologia. Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2018-11-06T13:17:29Z
dc.date.available.fl_str_mv 2018-11-06T13:17:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Glauber de S. et al. Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells. Plos One, v. 7, n. 1, e29561, p. 1-11, Jan. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29890
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0029561
identifier_str_mv ARAUJO, Glauber de S. et al. Capsules from Pathogenic and Non-Pathogenic Cryptococcus spp. Manifest Significant Differences in Structure and Ability to Protect against Phagocytic Cells. Plos One, v. 7, n. 1, e29561, p. 1-11, Jan. 2012.
1932-6203
10.1371/journal.pone.0029561
url https://www.arca.fiocruz.br/handle/icict/29890
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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