The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10540 |
Resumo: | Universidade do Estado do Rio de Janeiro (UERJ). Hospital Universitário Pedro Ernesto. Unidade de Cuidados Intensivos. Rio de Janeiro, RJ, Brasil. |
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Andrade, José Antenor Araújo deGayer, Carlos Roberto MachadoNogueira, Natália Pereira de AlmeidaPaes, Márcia CristinaBastos, Vera Lúcia CunhaBastos Neto, Jayme da CunhaAlves Jr., Sílvio CaetanoCoelho, Raphael MolinaroCunha, Mariana Gysele Amarante Teixeira daGomes, Rachel NovaesÁguila, Márcia BarbosaLacerda, Carlos Alberto Mandarim deBozza, Patrícia T.2015-05-27T13:39:48Z2015-05-27T13:39:48Z2014ANDRADE, José Antenor Araújo de et al. The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis. Journal Of Inflammation, v.11:11, 10p, 2014.1476-9255https://www.arca.fiocruz.br/handle/icict/1054010.1186/1476-9255-11-11engBioMed CentralThe effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro (UERJ). Hospital Universitário Pedro Ernesto. Unidade de Cuidados Intensivos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ).Instituto de Biologia. Cenrto Biomédico. Laboratório de Morfometria, Metabolismo e Cardiovascular. Rio de Janeiro, RJ, Brasil.Background Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model. Methods The experimental sepsis model, cecal ligation and puncture (CLP), was utilized on mice in comparison with a sham procedure. The following four groups were compared against each other: SHAM with AIN93G complete chow, SHAM with thiamine deficient (TD) chow, CLP with AIN93G complete chow, and CLP with TD chow. Thiamine pyrophosphate (TPP) blood concentrations were determined, and blood and peritoneal fluid were evaluated for differences in TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 levels. In addition, the levels of 4-HNE adducts in liver proteins were evaluated by Western Blot. Results The mean TPP blood concentration from the mice fed with the complete chow was 303.3 ± 42.6 nmol/L, and TD occurred within 10 days. TNF-α and MCP-1 concentrations in the peritoneal fluid were significantly greater in the CLP with TD chow group when compared with the other groups. The blood IL-1β level, however, was lower in the CLP with TD chow group. Liver 4-HNE levels were highest in the TD chow groups. Blood mononuclear cell numbers, as well as peritoneal total leukocyte, mononuclear cell and neutrophil numbers were greater in the CLP with TD chow group. Peritoneal bacterial colony forming units (CFU) were significantly lower in the CLP with TD chow group. Conclusion TD was associated with greater bacterial clearance, oxidative stress and inflammatory response changes.ThiamineSepsisSepsis modelInflammationOxidative stressEstresse OxidativoSepseTiaminainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10540/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALraphael_coelhoetal_IOC_2014.pdfapplication/pdf857144https://www.arca.fiocruz.br/bitstream/icict/10540/2/raphael_coelhoetal_IOC_2014.pdf7c889baaa931dc516ab12d72c17aa8efMD52TEXTraphael_coelhoetal_IOC_2014.pdf.txtraphael_coelhoetal_IOC_2014.pdf.txtExtracted texttext/plain43493https://www.arca.fiocruz.br/bitstream/icict/10540/3/raphael_coelhoetal_IOC_2014.pdf.txt08ea7819fc0aac3d9d42f1b7a4faa123MD53icict/105402022-06-24 12:17:34.319oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| title |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| spellingShingle |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis Andrade, José Antenor Araújo de Thiamine Sepsis Sepsis model Inflammation Oxidative stress Estresse Oxidativo Sepse Tiamina |
| title_short |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| title_full |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| title_fullStr |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| title_full_unstemmed |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| title_sort |
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis |
| author |
Andrade, José Antenor Araújo de |
| author_facet |
Andrade, José Antenor Araújo de Gayer, Carlos Roberto Machado Nogueira, Natália Pereira de Almeida Paes, Márcia Cristina Bastos, Vera Lúcia Cunha Bastos Neto, Jayme da Cunha Alves Jr., Sílvio Caetano Coelho, Raphael Molinaro Cunha, Mariana Gysele Amarante Teixeira da Gomes, Rachel Novaes Águila, Márcia Barbosa Lacerda, Carlos Alberto Mandarim de Bozza, Patrícia T. |
| author_role |
author |
| author2 |
Gayer, Carlos Roberto Machado Nogueira, Natália Pereira de Almeida Paes, Márcia Cristina Bastos, Vera Lúcia Cunha Bastos Neto, Jayme da Cunha Alves Jr., Sílvio Caetano Coelho, Raphael Molinaro Cunha, Mariana Gysele Amarante Teixeira da Gomes, Rachel Novaes Águila, Márcia Barbosa Lacerda, Carlos Alberto Mandarim de Bozza, Patrícia T. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Andrade, José Antenor Araújo de Gayer, Carlos Roberto Machado Nogueira, Natália Pereira de Almeida Paes, Márcia Cristina Bastos, Vera Lúcia Cunha Bastos Neto, Jayme da Cunha Alves Jr., Sílvio Caetano Coelho, Raphael Molinaro Cunha, Mariana Gysele Amarante Teixeira da Gomes, Rachel Novaes Águila, Márcia Barbosa Lacerda, Carlos Alberto Mandarim de Bozza, Patrícia T. |
| dc.subject.en.pt_BR.fl_str_mv |
Thiamine Sepsis Sepsis model Inflammation Oxidative stress |
| topic |
Thiamine Sepsis Sepsis model Inflammation Oxidative stress Estresse Oxidativo Sepse Tiamina |
| dc.subject.decs.pt_BR.fl_str_mv |
Estresse Oxidativo Sepse Tiamina |
| description |
Universidade do Estado do Rio de Janeiro (UERJ). Hospital Universitário Pedro Ernesto. Unidade de Cuidados Intensivos. Rio de Janeiro, RJ, Brasil. |
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2014 |
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2014 |
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2015-05-27T13:39:48Z |
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2015-05-27T13:39:48Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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ANDRADE, José Antenor Araújo de et al. The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis. Journal Of Inflammation, v.11:11, 10p, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10540 |
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1476-9255 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/1476-9255-11-11 |
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ANDRADE, José Antenor Araújo de et al. The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis. Journal Of Inflammation, v.11:11, 10p, 2014. 1476-9255 10.1186/1476-9255-11-11 |
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BioMed Central |
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BioMed Central |
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