Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions

Detalhes bibliográficos
Autor(a) principal: Gruenberg, Maria
Data de Publicação: 2018
Outros Autores: Moniz, Clara Antunes, Hofmann, Natalie Ellen, Wampfler, Rahel, Koepfli, Cristian, Mueller, Ivo, Monteiro, Wuelton Marcelo, Lacerda, Marcus, Melo, Gisely Cardoso de, Kuehn, Andrea, Siqueira, Andre M., Felger, Ingrid
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32342
Resumo: Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.
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spelling Gruenberg, MariaMoniz, Clara AntunesHofmann, Natalie EllenWampfler, RahelKoepfli, CristianMueller, IvoMonteiro, Wuelton MarceloLacerda, MarcusMelo, Gisely Cardoso deKuehn, AndreaSiqueira, Andre M.Felger, Ingrid2019-04-03T02:17:25Z2019-04-03T02:17:25Z2018GRUENBERG, Maria et al. Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions. Malaria Journal, v. 17, n. 55, p. 1-10, 2018.1475-2875https://www.arca.fiocruz.br/handle/icict/3234210.1186/s12936-018-2201-01475-2875engBMCPlasmodium vivax molecular diagnostics in community surveys: pitfalls and solutionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSwiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.Walter and Eliza Hall Institute of Medical Research. Parkville, VIC, Australia.Walter and Eliza Hall Institute of Medical Research. Parkville, VIC, Australia.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universidade do Estado do Amazonas, Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universidade do Estado do Amazonas, Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.A distinctive feature of Plasmodium vivax infections is the overall low parasite density in peripheral blood. Thus, identifying asymptomatic infected individuals in endemic communities requires diagnostic tests with high sensitivity. The detection limits of molecular diagnostic tests are primarily defined by the volume of blood analysed and by the copy number of the amplified molecular marker serving as the template for amplification. By using mitochondrial DNA as the multi-copy template, the detection limit can be improved more than tenfold, compared to standard 18S rRNA targets, thereby allowing detection of lower parasite densities. In a very low transmission area in Brazil, application of a mitochondrial DNA-based assay increased prevalence from 4.9 to 6.5%. The usefulness of molecular tests in malaria epidemiological studies is widely recognized, especially when precise prevalence rates are desired. Of concern, however, is the challenge of demonstrating test accuracy and quality control for samples with very low parasite densities. In this case, chance effects in template distribution around the detection limit constrain reproducibility. Rigorous assessment of false positive and false negative test results is, therefore, required to prevent over- or under-estimation of parasite prevalence in epidemiological studies or when monitoring interventions.Plasmodium vivaxSurveillanceMolecular diagnosticsMitochondrial DNA18S rRNA transcriptsLAMPQuantifcationGametocytesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32342/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Gruenberg_Maria_etal_INI_2018.pdfve_Gruenberg_Maria_etal_INI_2018.pdfapplication/pdf1598266https://www.arca.fiocruz.br/bitstream/icict/32342/2/ve_Gruenberg_Maria_etal_INI_2018.pdfed052e5637a8f21e14b7815c861b56b3MD52TEXTve_Gruenberg_Maria_etal_INI_2018.pdf.txtve_Gruenberg_Maria_etal_INI_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
title Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
spellingShingle Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
Gruenberg, Maria
Plasmodium vivax
Surveillance
Molecular diagnostics
Mitochondrial DNA
18S rRNA transcripts
LAMP
Quantifcation
Gametocytes
title_short Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
title_full Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
title_fullStr Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
title_full_unstemmed Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
title_sort Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
author Gruenberg, Maria
author_facet Gruenberg, Maria
Moniz, Clara Antunes
Hofmann, Natalie Ellen
Wampfler, Rahel
Koepfli, Cristian
Mueller, Ivo
Monteiro, Wuelton Marcelo
Lacerda, Marcus
Melo, Gisely Cardoso de
Kuehn, Andrea
Siqueira, Andre M.
Felger, Ingrid
author_role author
author2 Moniz, Clara Antunes
Hofmann, Natalie Ellen
Wampfler, Rahel
Koepfli, Cristian
Mueller, Ivo
Monteiro, Wuelton Marcelo
Lacerda, Marcus
Melo, Gisely Cardoso de
Kuehn, Andrea
Siqueira, Andre M.
Felger, Ingrid
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gruenberg, Maria
Moniz, Clara Antunes
Hofmann, Natalie Ellen
Wampfler, Rahel
Koepfli, Cristian
Mueller, Ivo
Monteiro, Wuelton Marcelo
Lacerda, Marcus
Melo, Gisely Cardoso de
Kuehn, Andrea
Siqueira, Andre M.
Felger, Ingrid
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Surveillance
Molecular diagnostics
Mitochondrial DNA
18S rRNA transcripts
LAMP
Quantifcation
Gametocytes
topic Plasmodium vivax
Surveillance
Molecular diagnostics
Mitochondrial DNA
18S rRNA transcripts
LAMP
Quantifcation
Gametocytes
description Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-04-03T02:17:25Z
dc.date.available.fl_str_mv 2019-04-03T02:17:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GRUENBERG, Maria et al. Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions. Malaria Journal, v. 17, n. 55, p. 1-10, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32342
dc.identifier.issn.pt_BR.fl_str_mv 1475-2875
dc.identifier.doi.none.fl_str_mv 10.1186/s12936-018-2201-0
dc.identifier.eissn.none.fl_str_mv 1475-2875
identifier_str_mv GRUENBERG, Maria et al. Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions. Malaria Journal, v. 17, n. 55, p. 1-10, 2018.
1475-2875
10.1186/s12936-018-2201-0
url https://www.arca.fiocruz.br/handle/icict/32342
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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