Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis

Detalhes bibliográficos
Autor(a) principal: Totino, Paulo Renato Rivas
Data de Publicação: 2014
Outros Autores: Magalhães, Aline das Dores, Alves, Eliana Brasil, Costa, Monica Regina Farias, Lacerda, Marcus Vinicius Guimarães de, Daniel-Ribeiro, Cláudio Tadeu, Cruz, Maria de Fátima Ferreira da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10688
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.
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spelling Totino, Paulo Renato RivasMagalhães, Aline das DoresAlves, Eliana BrasilCosta, Monica Regina FariasLacerda, Marcus Vinicius Guimarães deDaniel-Ribeiro, Cláudio TadeuCruz, Maria de Fátima Ferreira da2015-06-08T14:01:49Z2015-06-08T14:01:49Z2014TOTINO, Paulo Renato Rivas et al. Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis. Parasite & Vectors, v.7:484, 6p, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/1068810.1186/s13071-014-0484-8engBioMed CentralPlasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.Fundação de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.Apoptosis can occur in red blood cells (RBC) and seems to be involved in hematologic disorders related to many diseases. In malaria it is known that parasitized RBC (pRBC) is involved in the development of anemia and thrombosis; however, non-parasitized RBC (nRBC) apoptosis could amplify these malaria-associated hematologic events. In fact, in experimental malaria, increased levels of apoptosis were observed in nRBC during lethal Plasmodium yoelii 17XL infection, but in human malaria erythrocytic apoptosis has never been studied. The present study was performed to investigate if nRBC apoptosis also occurs in P. vivax and P. falciparum infections. Findings Apoptosis of nRBC was evaluated in blood samples of P. vivax malaria patients and clinically healthly individuals living in Manaus, Brazil, both ex vivo and after incubation of RBC for 24 h. Additionally, the capacity of plasma from P. vivax or P. falciparum patients was tested for induction of in vitro apoptosis of normal RBC from a clinically healthy individual living in a non-endemic malaria region. Apoptosis was detected by flow cytometry using annexin V staining. In contrast to experimental malaria that significantly increased the levels of apoptotic nRBC both ex-vivo and after 24 h of incubation, no significant alteration on apoptotic nRBC rates was detected in P. vivax infected patients when compared with non-infected control individuals. Similar results were observed when plasma of these P. vivax patients was incubated with normal RBC. Conversely, plasma from P. falciparum-infected subjects induced significant apoptosis of these cells. Conclusions Apoptosis of normal RBC can be induced by plasma from individuals with P. falciparum (but not with P. vivax) malaria. This finding could reflect the existence of erythrocytic apoptosis during infection that could contribute to the pathogenesis of hematological and vascular complications associated with falciparum malaria.Plasmodium falciparumApoptosisMalariaApoptoseMaláriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10688/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALpaulo_totinoetal_IOC_2014.pdfapplication/pdf669417https://www.arca.fiocruz.br/bitstream/icict/10688/2/paulo_totinoetal_IOC_2014.pdfadb0470e2cafc072cfdf7600426adb22MD52TEXTpaulo_totinoetal_IOC_2014.pdf.txtpaulo_totinoetal_IOC_2014.pdf.txtExtracted texttext/plain24121https://www.arca.fiocruz.br/bitstream/icict/10688/3/paulo_totinoetal_IOC_2014.pdf.txt95c58dd8f5fe74cb2dd2f02670f3c7f5MD53icict/106882022-06-24 13:12:32.779oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
title Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
spellingShingle Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
Totino, Paulo Renato Rivas
Plasmodium falciparum
Apoptosis
Malaria
Apoptose
Malária
title_short Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
title_full Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
title_fullStr Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
title_full_unstemmed Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
title_sort Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
author Totino, Paulo Renato Rivas
author_facet Totino, Paulo Renato Rivas
Magalhães, Aline das Dores
Alves, Eliana Brasil
Costa, Monica Regina Farias
Lacerda, Marcus Vinicius Guimarães de
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
author_role author
author2 Magalhães, Aline das Dores
Alves, Eliana Brasil
Costa, Monica Regina Farias
Lacerda, Marcus Vinicius Guimarães de
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Totino, Paulo Renato Rivas
Magalhães, Aline das Dores
Alves, Eliana Brasil
Costa, Monica Regina Farias
Lacerda, Marcus Vinicius Guimarães de
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
dc.subject.en.pt_BR.fl_str_mv Plasmodium falciparum
Apoptosis
Malaria
topic Plasmodium falciparum
Apoptosis
Malaria
Apoptose
Malária
dc.subject.decs.pt_BR.fl_str_mv Apoptose
Malária
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Brasília, DF, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:49Z
dc.date.available.fl_str_mv 2015-06-08T14:01:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TOTINO, Paulo Renato Rivas et al. Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis. Parasite & Vectors, v.7:484, 6p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10688
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s13071-014-0484-8
identifier_str_mv TOTINO, Paulo Renato Rivas et al. Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis. Parasite & Vectors, v.7:484, 6p, 2014.
1756-3305
10.1186/s13071-014-0484-8
url https://www.arca.fiocruz.br/handle/icict/10688
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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