Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.

Detalhes bibliográficos
Autor(a) principal: Pereira, Priscilla Aparecida Tartari
Data de Publicação: 2015
Outros Autores: Bitencourt, Claudia da Silva, Santos, Daiane Fernanda dos, Nicolete, Roberto, Gelfuso, Guilherme Martins, Faccioli, Lúcia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12628
Resumo: 2016-11-30
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spelling Pereira, Priscilla Aparecida TartariBitencourt, Claudia da SilvaSantos, Daiane Fernanda dosNicolete, RobertoGelfuso, Guilherme MartinsFaccioli, Lúcia Helena2016-01-26T18:17:50Z2015NICOLETE, R. et al. Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions. European Journal of Pharmaceutical Sciences, p.132-139, 2015.https://www.arca.fiocruz.br/handle/icict/1262810.1016/j.ejps.2015.07.001engELSEVIERProstaglandin D2-loaded microspheres effectively activate macrophage effector functions.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-11-30Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Universidade de Brasília. Faculdade de Ciências da Saúde. Laboratório de Tecnologia de Medicamentos, Alimentos e Cosméticos. Brasília, DF, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Biodegradable lactic-co-glycolic acid (PLGA) microspheres (MS) improve the stability of biomolecules stability and allow enable their sustained release. Lipid mediators represent a strategy for improving host defense; however, most of these mediators, such as prostaglandin D2 (PGD2), have low water solubility and are unstable. The present study aimed to develop and characterize MS loaded with PGD2 (PGD2-MS) to obtain an innovative tool to activate macrophages. PGD2-MS were prepared using an oil-in-water emulsion solvent extraction-evaporation process, and the size, zeta potential, surface morphology and encapsulation efficiency were determined. It was also evaluated in vitro the phagocytic index, NF-jB activation, as well as nitric oxide and cytokine production by alveolar macrophages (AMs) in response to PGD2-MS. PGD2-MS were spherical with a diameter of 5.0 ± 3.3 lm and regular surface, zeta potential of ÿ13.4 ± 5.6 mV, and 36% of encapsulation efficiency, with 16–26% release of entrapped PGD2 at 4 and 48 h, respectively. PGD2-MS were more efficiently internalized by AMs than unloaded-MS, and activated NF-jB more than free PGD2. Moreover, PGD2-MS stimulated the production of nitric oxide, TNF-a, IL-1b, and TGF-b, more than free PGD2, indicating that microencapsulation increased the activating effect of PGD2 on cells. In LPS-pre-treated AMs, PGD2-MS decreased the release of IL-6 but increased the production of nitric oxide and IL-1b. These results show that the morphological characteristics of PGD2-MS facilitated interaction with, and activation of phagocytic cells; moreover, PGD2-MS retained the biological activities of PGD2 to trigger effector mechanisms in AMs. It is suggested that PGD2-MS represent a strategy for therapeutic intervention in the lungs of immunocompromised subjects.MicrospheresPGD2Drug deliveryPhagocytosisCytokinesAlveolar macrophagesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12628/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALProstaglandin D2-loaded microspheres.pdfProstaglandin D2-loaded microspheres.pdfapplication/pdf882119https://www.arca.fiocruz.br/bitstream/icict/12628/2/Prostaglandin%20D2-loaded%20microspheres.pdf927b312f5e22149c89e0086316be8223MD52TEXTProstaglandin D2-loaded microspheres.pdf.txtProstaglandin D2-loaded microspheres.pdf.txtExtracted texttext/plain39716https://www.arca.fiocruz.br/bitstream/icict/12628/3/Prostaglandin%20D2-loaded%20microspheres.pdf.txt311b441022fea4a0aba6dba8a0ef46e2MD53icict/126282021-02-10 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dc.title.pt_BR.fl_str_mv Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
title Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
spellingShingle Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
Pereira, Priscilla Aparecida Tartari
Microspheres
PGD2
Drug delivery
Phagocytosis
Cytokines
Alveolar macrophages
title_short Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
title_full Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
title_fullStr Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
title_full_unstemmed Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
title_sort Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions.
author Pereira, Priscilla Aparecida Tartari
author_facet Pereira, Priscilla Aparecida Tartari
Bitencourt, Claudia da Silva
Santos, Daiane Fernanda dos
Nicolete, Roberto
Gelfuso, Guilherme Martins
Faccioli, Lúcia Helena
author_role author
author2 Bitencourt, Claudia da Silva
Santos, Daiane Fernanda dos
Nicolete, Roberto
Gelfuso, Guilherme Martins
Faccioli, Lúcia Helena
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Priscilla Aparecida Tartari
Bitencourt, Claudia da Silva
Santos, Daiane Fernanda dos
Nicolete, Roberto
Gelfuso, Guilherme Martins
Faccioli, Lúcia Helena
dc.subject.en.pt_BR.fl_str_mv Microspheres
PGD2
Drug delivery
Phagocytosis
Cytokines
Alveolar macrophages
topic Microspheres
PGD2
Drug delivery
Phagocytosis
Cytokines
Alveolar macrophages
description 2016-11-30
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-26T18:17:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NICOLETE, R. et al. Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions. European Journal of Pharmaceutical Sciences, p.132-139, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12628
dc.identifier.doi.none.fl_str_mv 10.1016/j.ejps.2015.07.001
identifier_str_mv NICOLETE, R. et al. Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions. European Journal of Pharmaceutical Sciences, p.132-139, 2015.
10.1016/j.ejps.2015.07.001
url https://www.arca.fiocruz.br/handle/icict/12628
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12628/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12628/2/Prostaglandin%20D2-loaded%20microspheres.pdf
https://www.arca.fiocruz.br/bitstream/icict/12628/3/Prostaglandin%20D2-loaded%20microspheres.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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