Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients

Detalhes bibliográficos
Autor(a) principal: Queiroz, Gabriel Andrade Nonato
Data de Publicação: 2019
Outros Autores: Mascarenhas, Rita Elizabeth Moreira, Vieillard, Vincent, Andrade, Raphaela Lisboa, Castro Filho, Bernardo Galvão, Grassi, Maria Fernanda Rios
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34645
Resumo: Fundação de Amparo a Pesquisa da Bahia (FAPESB) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Grassi, M.F.R. and Galvão-Castro, B, are currently receiving scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from Fundação Nacional de Desenvolvimento do Ensino Superior Particular (Funadesp).
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spelling Queiroz, Gabriel Andrade NonatoMascarenhas, Rita Elizabeth MoreiraVieillard, VincentAndrade, Raphaela LisboaCastro Filho, Bernardo GalvãoGrassi, Maria Fernanda Rios2019-08-07T13:03:18Z2019-08-07T13:03:18Z2019QUEIROZ, Gabriel Andrade Nonato et al. Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. BMC Infectious Diseases, v. 19, p. 1-9, 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/3464510.1186/s12879-019-4032-1Fundação de Amparo a Pesquisa da Bahia (FAPESB) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Grassi, M.F.R. and Galvão-Castro, B, are currently receiving scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from Fundação Nacional de Desenvolvimento do Ensino Superior Particular (Funadesp).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Sorbonne Universités. Centre d’Immunologie et des Maladies Infectieuses. Paris, France.Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Natural killer (NK) cells are part of the innate immune system and provide surveillance against viruses and cancers. The ability of NK cells to kill virus-infected cells depends on the balance between the effects of inhibitory and activating NK cell receptors. This study aimed to investigate the phenotypic profile and the functional capacity of NK cells in the context of HTLV-1 infection. Methods: This cross-sectional study sequentially recruited HTLV-1 infected individuals with HTLV-1 associated myelopathy/ tropical spastic paraparesis (HAM/TSP) and asymptomatic HTLV-1 (AS) from the Integrated and Multidisciplinary HTLV Center in Salvador, Brazil. Blood samples from healthy blood donors served as controls. NK cell surface receptors (NKG2D, KIR2DL2/KIR2DL3, NKp30, NKG2A, NKp46, TIM-3 and PD-1), intracellular cytolytic (Granzyme B, perforin) and functional markers (CD107a for degranulation, IFN-γ) were assayed by flow cytometry in the presence or absence of standard K562 target cells. In addition, cytotoxicity assays were performed in the presence or absence of anti-NKp30. Results: The frequency of NKp30+ NK cells was significantly decreased in HAM/TSP patients [58%, Interquartile Range (IQR) 30–61] compared to controls (73%, IQR 54–79, p = 0.04). The production of cytolytic (perforin, granzyme B) and functional markers (CD107a and IFN-γ) was higher in unstimulated NK cells from HAM/TSP and AS patients compared to controls. By contrast, stimulation with K562 target cells did not alter the frequency of CD107a+ NK cells in HAM/TSP subjects compared to the other groups. Blockage of the NKp30 receptor was shown to decrease cytotoxic activity (CD107a) and IFN-γ expression only in asymptomatic HTLV-1-infected individuals. Conclusions: NK cells from individuals with a diagnosis of HAM/TSP present decreased expression of the activating receptor NKp30, in addition to elevated degranulation activity that remained unaffected after blocking the NKp30 receptor.engBMCCélulas NKNKp30Receptor de citotoxicidade naturalCD107HTLV-1HAM / TSPNK cellsNKp30Natural cytotoxicity receptorCD107HTLV-1HAM/TSPFunctional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34645/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALQueiroz, G.A. Functional capacity...2019.pdfQueiroz, G.A. Functional capacity...2019.pdfapplication/pdf2446462https://www.arca.fiocruz.br/bitstream/icict/34645/2/Queiroz%2c%20G.A.%20Functional%20capacity...2019.pdf6c537e9261b024afed487589b9badee6MD52TEXTQueiroz, G.A. Functional capacity...2019.pdf.txtQueiroz, G.A. 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dc.title.pt_BR.fl_str_mv Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
title Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
spellingShingle Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
Queiroz, Gabriel Andrade Nonato
Células NK
NKp30
Receptor de citotoxicidade natural
CD107
HTLV-1
HAM / TSP
NK cells
NKp30
Natural cytotoxicity receptor
CD107
HTLV-1
HAM/TSP
title_short Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
title_full Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
title_fullStr Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
title_full_unstemmed Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
title_sort Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients
author Queiroz, Gabriel Andrade Nonato
author_facet Queiroz, Gabriel Andrade Nonato
Mascarenhas, Rita Elizabeth Moreira
Vieillard, Vincent
Andrade, Raphaela Lisboa
Castro Filho, Bernardo Galvão
Grassi, Maria Fernanda Rios
author_role author
author2 Mascarenhas, Rita Elizabeth Moreira
Vieillard, Vincent
Andrade, Raphaela Lisboa
Castro Filho, Bernardo Galvão
Grassi, Maria Fernanda Rios
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Gabriel Andrade Nonato
Mascarenhas, Rita Elizabeth Moreira
Vieillard, Vincent
Andrade, Raphaela Lisboa
Castro Filho, Bernardo Galvão
Grassi, Maria Fernanda Rios
dc.subject.other.pt_BR.fl_str_mv Células NK
NKp30
Receptor de citotoxicidade natural
CD107
HTLV-1
HAM / TSP
topic Células NK
NKp30
Receptor de citotoxicidade natural
CD107
HTLV-1
HAM / TSP
NK cells
NKp30
Natural cytotoxicity receptor
CD107
HTLV-1
HAM/TSP
dc.subject.en.pt_BR.fl_str_mv NK cells
NKp30
Natural cytotoxicity receptor
CD107
HTLV-1
HAM/TSP
description Fundação de Amparo a Pesquisa da Bahia (FAPESB) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Grassi, M.F.R. and Galvão-Castro, B, are currently receiving scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from Fundação Nacional de Desenvolvimento do Ensino Superior Particular (Funadesp).
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-07T13:03:18Z
dc.date.available.fl_str_mv 2019-08-07T13:03:18Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Gabriel Andrade Nonato et al. Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. BMC Infectious Diseases, v. 19, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34645
dc.identifier.issn.pt_BR.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-019-4032-1
identifier_str_mv QUEIROZ, Gabriel Andrade Nonato et al. Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. BMC Infectious Diseases, v. 19, p. 1-9, 2019.
1471-2334
10.1186/s12879-019-4032-1
url https://www.arca.fiocruz.br/handle/icict/34645
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dc.publisher.none.fl_str_mv BMC
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