Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole

Detalhes bibliográficos
Autor(a) principal: Cruz, Agostinho
Data de Publicação: 2003
Outros Autores: Sousa, M. Isaura, Azeredo, Zaida, Leite, Emilia, Sousa, J. C. Figueiredo de, Cabral, Miguel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/23155
Resumo: From 53 samples of human faeces containing Giardia lamblia cysts, 18 isolates were successfully excysted in vitro, and cultivated axenically in TYI-S-33 modified medium. The in vitro effects of metronidazole and albendazole on these isolates were evaluated by the trophozoite adherence inhibition method. The IC50 was between 2.4 and 11.5 µM for metronidazole and 0.027 and 0.192 µM for albendazole. These IC50 values were similar to those found for the ATCC 30888 and 30957 reference isolates. All isolates were susceptible to the antiparasitic drugs tested. These results suggest that resistance of G. lamblia to metronidazole and albendazole does not seem to be a significant problem in our population.
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spelling Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazoleGiardiaSusceptibility assaysMetronidazoleAlbendazoleFrom 53 samples of human faeces containing Giardia lamblia cysts, 18 isolates were successfully excysted in vitro, and cultivated axenically in TYI-S-33 modified medium. The in vitro effects of metronidazole and albendazole on these isolates were evaluated by the trophozoite adherence inhibition method. The IC50 was between 2.4 and 11.5 µM for metronidazole and 0.027 and 0.192 µM for albendazole. These IC50 values were similar to those found for the ATCC 30888 and 30957 reference isolates. All isolates were susceptible to the antiparasitic drugs tested. These results suggest that resistance of G. lamblia to metronidazole and albendazole does not seem to be a significant problem in our population.Oxford AcademicRepositório Científico do Instituto Politécnico do PortoCruz, AgostinhoSousa, M. IsauraAzeredo, ZaidaLeite, EmiliaSousa, J. C. Figueiredo deCabral, Miguel2023-06-16T11:00:10Z2003-04-012003-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/23155engCruz, A., Sousa, M. I., Azeredo, Z., Leite, E., Figueiredo de Sousa, J. C., & Cabral, M. (2003). Isolation, excystation and axenization of Giardia lamblia isolates: In vitro susceptibility to metronidazole and albendazole. Journal of Antimicrobial Chemotherapy, 51(4), 1017–1020. https://doi.org/10.1093/jac/dkg1500305-745310.1093/jac/dkg1501460-2091info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-06-21T01:46:17Zoai:recipp.ipp.pt:10400.22/23155Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:01:08.778410Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
title Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
spellingShingle Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
Cruz, Agostinho
Giardia
Susceptibility assays
Metronidazole
Albendazole
title_short Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
title_full Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
title_fullStr Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
title_full_unstemmed Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
title_sort Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole
author Cruz, Agostinho
author_facet Cruz, Agostinho
Sousa, M. Isaura
Azeredo, Zaida
Leite, Emilia
Sousa, J. C. Figueiredo de
Cabral, Miguel
author_role author
author2 Sousa, M. Isaura
Azeredo, Zaida
Leite, Emilia
Sousa, J. C. Figueiredo de
Cabral, Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Cruz, Agostinho
Sousa, M. Isaura
Azeredo, Zaida
Leite, Emilia
Sousa, J. C. Figueiredo de
Cabral, Miguel
dc.subject.por.fl_str_mv Giardia
Susceptibility assays
Metronidazole
Albendazole
topic Giardia
Susceptibility assays
Metronidazole
Albendazole
description From 53 samples of human faeces containing Giardia lamblia cysts, 18 isolates were successfully excysted in vitro, and cultivated axenically in TYI-S-33 modified medium. The in vitro effects of metronidazole and albendazole on these isolates were evaluated by the trophozoite adherence inhibition method. The IC50 was between 2.4 and 11.5 µM for metronidazole and 0.027 and 0.192 µM for albendazole. These IC50 values were similar to those found for the ATCC 30888 and 30957 reference isolates. All isolates were susceptible to the antiparasitic drugs tested. These results suggest that resistance of G. lamblia to metronidazole and albendazole does not seem to be a significant problem in our population.
publishDate 2003
dc.date.none.fl_str_mv 2003-04-01
2003-04-01T00:00:00Z
2023-06-16T11:00:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/23155
url http://hdl.handle.net/10400.22/23155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cruz, A., Sousa, M. I., Azeredo, Z., Leite, E., Figueiredo de Sousa, J. C., & Cabral, M. (2003). Isolation, excystation and axenization of Giardia lamblia isolates: In vitro susceptibility to metronidazole and albendazole. Journal of Antimicrobial Chemotherapy, 51(4), 1017–1020. https://doi.org/10.1093/jac/dkg150
0305-7453
10.1093/jac/dkg150
1460-2091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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