Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain

Detalhes bibliográficos
Autor(a) principal: Santos, Camila Ramos dos
Data de Publicação: 2009
Outros Autores: Fessel, Melissa Regina, Vieira, Leandro de Carvalho, Krieger, Marco Aurélio, Goldenberg, Samuel, Guimarães, Beatriz Gomes, Zanchin, Nilson Ivo Tonin, Barbosa, João Alexandre Ribeiro Gonçalves
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1107/S1744309109017734
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40105
Resumo: Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.
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spelling Santos, Camila Ramos dosFessel, Melissa ReginaVieira, Leandro de CarvalhoKrieger, Marco AurélioGoldenberg, SamuelGuimarães, Beatriz GomesZanchin, Nilson Ivo ToninBarbosa, João Alexandre Ribeiro Gonçalves2020-02-20T14:22:52Z2020-02-20T14:22:52Z2009SANTOS, Camila Ramos dos et al. Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v. 6, n. 6, p. 1-9, 2011.1744-3091https://www.arca.fiocruz.br/handle/icict/4010510.1107/S17443091090177341744-3091porInternational Union of CrystallographyTrypanosoma cruziThioredoxinsGene ExpressionGenetic StructuresExpresión GénicaEstructuras GenéticasTiorredoxinasExpressão GênicaEstruturas GenéticasCrystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domaininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Synchrotron SOLEIL. Saint-Aubin, France.Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.Q4DV70 is annotated in the Trypanosoma cruzi CL Brener genome as a hypothetical protein with a predicted thioredoxin-like fold, although the catalytic cysteine residues that are conserved in typical oxidoreductases are replaced by serine residues. Gene-expression analysis indicates that this protein is differentially expressed during the T. cruzi life cycle, suggesting that it plays an important role during T. cruzi development. The gene coding for Q4DV70 was cloned and the protein was overexpressed in Escherichia coli with an N-terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method. A diffraction data set was collected to 1.50 A resolution from a single crystal grown in 25% PEG 1500, 200 mM sodium thiocyanate pH 6.9, 10 mM phenol and 10% ethylene glycol. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 35.04, b = 50.32, c = 61.18 A. The Q4DV70 structure was solved by molecular replacement using protein disulfide isomerase from yeast (PDB code 2b5e) as a search model. Initial refinement of the model indicated that the solution was correct. These data are being used for refinement of the model of Q4DV70.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40105/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALS1744309109017734ok.pdfS1744309109017734ok.pdfapplication/pdf234664https://www.arca.fiocruz.br/bitstream/icict/40105/2/S1744309109017734ok.pdfb5b2a9f3385ce182c87ece0db45d1346MD52TEXTS1744309109017734ok.pdf.txtS1744309109017734ok.pdf.txtExtracted texttext/plain19998https://www.arca.fiocruz.br/bitstream/icict/40105/3/S1744309109017734ok.pdf.txt81b9243376a8e0f8434921bb25350878MD53icict/401052020-02-25 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dc.title.pt_BR.fl_str_mv Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
title Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
spellingShingle Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
Santos, Camila Ramos dos
Trypanosoma cruzi
Thioredoxins
Gene Expression
Genetic Structures
Expresión Génica
Estructuras Genéticas
Tiorredoxinas
Expressão Gênica
Estruturas Genéticas
title_short Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
title_full Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
title_fullStr Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
title_full_unstemmed Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
title_sort Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain
author Santos, Camila Ramos dos
author_facet Santos, Camila Ramos dos
Fessel, Melissa Regina
Vieira, Leandro de Carvalho
Krieger, Marco Aurélio
Goldenberg, Samuel
Guimarães, Beatriz Gomes
Zanchin, Nilson Ivo Tonin
Barbosa, João Alexandre Ribeiro Gonçalves
author_role author
author2 Fessel, Melissa Regina
Vieira, Leandro de Carvalho
Krieger, Marco Aurélio
Goldenberg, Samuel
Guimarães, Beatriz Gomes
Zanchin, Nilson Ivo Tonin
Barbosa, João Alexandre Ribeiro Gonçalves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Camila Ramos dos
Fessel, Melissa Regina
Vieira, Leandro de Carvalho
Krieger, Marco Aurélio
Goldenberg, Samuel
Guimarães, Beatriz Gomes
Zanchin, Nilson Ivo Tonin
Barbosa, João Alexandre Ribeiro Gonçalves
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Thioredoxins
Gene Expression
Genetic Structures
Expresión Génica
Estructuras Genéticas
Tiorredoxinas
Expressão Gênica
Estruturas Genéticas
dc.subject.en.pt_BR.fl_str_mv Thioredoxins
Gene Expression
Genetic Structures
dc.subject.es.pt_BR.fl_str_mv Expresión Génica
Estructuras Genéticas
dc.subject.decs.pt_BR.fl_str_mv Tiorredoxinas
Expressão Gênica
Estruturas Genéticas
description Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-02-20T14:22:52Z
dc.date.available.fl_str_mv 2020-02-20T14:22:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Camila Ramos dos et al. Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v. 6, n. 6, p. 1-9, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40105
dc.identifier.issn.pt_BR.fl_str_mv 1744-3091
dc.identifier.doi.none.fl_str_mv 10.1107/S1744309109017734
dc.identifier.eissn.none.fl_str_mv 1744-3091
identifier_str_mv SANTOS, Camila Ramos dos et al. Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v. 6, n. 6, p. 1-9, 2011.
1744-3091
10.1107/S1744309109017734
url https://www.arca.fiocruz.br/handle/icict/40105
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv International Union of Crystallography
publisher.none.fl_str_mv International Union of Crystallography
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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