An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway

Detalhes bibliográficos
Autor(a) principal: Serpeloni, Mariana
Data de Publicação: 2011
Outros Autores: Moraes, Carolina Borsoi, Muniz, João Renato Carvalho, Motta, Maria Cristina Machado, Ramos, Augusto Savio Peixoto, Kessler, Rafael Luis, Inoue, Alexandre Haruo, Rocha, Wanderson Duarte da, Yamada-Ogatta, Sueli Fumie, Fragoso, Stenio Perdigão, Goldenberg, Samuel, Freitas-Junior, Lucio Holanda, Ávila, Andréa Rodrigues
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30033
Resumo: Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
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spelling Serpeloni, MarianaMoraes, Carolina BorsoiMuniz, João Renato CarvalhoMotta, Maria Cristina MachadoRamos, Augusto Savio PeixotoKessler, Rafael LuisInoue, Alexandre HaruoRocha, Wanderson Duarte daYamada-Ogatta, Sueli FumieFragoso, Stenio PerdigãoGoldenberg, SamuelFreitas-Junior, Lucio HolandaÁvila, Andréa Rodrigues2018-11-14T17:05:51Z2018-11-14T17:05:51Z2011SERPELONI, Mariana et al. An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway. PLoS ONE, v. 6, n. 6, p. 1-14, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/3003310.1371/journal.pone.00207301932-6203porPublic Library of ScienceAn essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathwayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Gyeonggi-do, South Korea.University of Oxford. Oxford. United Kingdom.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Departamento de Biologia Celular e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Gyeonggi-do, South Korea.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.In eukaryotic cells, different RNA species are exported from the nucleus via specialized pathways. The mRNA export machinery is highly integrated with mRNA processing, and includes a different set of nuclear transport adaptors as well as other mRNA binding proteins, RNA helicases, and NPC-associated proteins. The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, a widespread and neglected human disease which is endemic to Latin America. Gene expression in Trypanosoma has unique characteristics, such as constitutive polycistronic transcription of protein-encoding genes and mRNA processing by trans-splicing. In general, post-transcriptional events are the major points for regulation of gene expression in these parasites. However, the export pathway of mRNA from the nucleus is poorly understood. The present study investigated the function of TcSub2, which is a highly conserved protein ortholog to Sub2/ UAP56, a component of the Transcription/Export (TREX) multiprotein complex connecting transcription with mRNA export in yeast/human. Similar to its orthologs, TcSub2 is a nuclear protein, localized in dispersed foci all over the nuclei -except the fibrillar center of nucleolus- and at the interface between dense and non-dense chromatin areas, proposing the association of TcSub2 with transcription/processing sites. These findings were analyzed further by BrUTP incorporation assays and confirmed that TcSub2 is physically associated with active RNA polymerase II (RNA pol II), but not RNA polymerase I (RNA pol I) or Spliced Leader (SL) transcription, demonstrating participation particularly in nuclear mRNA metabolism in T. cruzi. The double knockout of the TcSub2 gene is lethal in T. cruzi, suggesting it has an essential function. Alternatively, RNA interference assays were performed in Trypanosoma brucei. It allowed demonstrating that besides being an essential protein, its knockdown causes mRNA accumulation in the nucleus and decrease of translation levels, reinforcing that Trypanosoma-Sub2 (Tryp-Sub2) is a component of mRNA transcription/export pathway in trypanosomes.RNA, MessengerTranscription GeneticGene ExpressionARN MensajeroTranscripción GenéticaExpresión GénicaRNA MensageiroTrypanosoma cruziTranscrição GenéticaTrypanosomaExpressão Gênicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30033/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALPlosone.002070.pdfPlosone.002070.pdfapplication/pdf1230885https://www.arca.fiocruz.br/bitstream/icict/30033/2/Plosone.002070.pdfd645d9076981d7428fccacf7765c722cMD52TEXTPlosone.002070.pdf.txtPlosone.002070.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
title An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
spellingShingle An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
Serpeloni, Mariana
RNA, Messenger
Transcription Genetic
Gene Expression
ARN Mensajero
Transcripción Genética
Expresión Génica
RNA Mensageiro
Trypanosoma cruzi
Transcrição Genética
Trypanosoma
Expressão Gênica
title_short An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
title_full An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
title_fullStr An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
title_full_unstemmed An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
title_sort An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
author Serpeloni, Mariana
author_facet Serpeloni, Mariana
Moraes, Carolina Borsoi
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Ramos, Augusto Savio Peixoto
Kessler, Rafael Luis
Inoue, Alexandre Haruo
Rocha, Wanderson Duarte da
Yamada-Ogatta, Sueli Fumie
Fragoso, Stenio Perdigão
Goldenberg, Samuel
Freitas-Junior, Lucio Holanda
Ávila, Andréa Rodrigues
author_role author
author2 Moraes, Carolina Borsoi
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Ramos, Augusto Savio Peixoto
Kessler, Rafael Luis
Inoue, Alexandre Haruo
Rocha, Wanderson Duarte da
Yamada-Ogatta, Sueli Fumie
Fragoso, Stenio Perdigão
Goldenberg, Samuel
Freitas-Junior, Lucio Holanda
Ávila, Andréa Rodrigues
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Serpeloni, Mariana
Moraes, Carolina Borsoi
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Ramos, Augusto Savio Peixoto
Kessler, Rafael Luis
Inoue, Alexandre Haruo
Rocha, Wanderson Duarte da
Yamada-Ogatta, Sueli Fumie
Fragoso, Stenio Perdigão
Goldenberg, Samuel
Freitas-Junior, Lucio Holanda
Ávila, Andréa Rodrigues
dc.subject.en.pt_BR.fl_str_mv RNA, Messenger
Transcription Genetic
Gene Expression
topic RNA, Messenger
Transcription Genetic
Gene Expression
ARN Mensajero
Transcripción Genética
Expresión Génica
RNA Mensageiro
Trypanosoma cruzi
Transcrição Genética
Trypanosoma
Expressão Gênica
dc.subject.es.pt_BR.fl_str_mv ARN Mensajero
Transcripción Genética
Expresión Génica
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro
Trypanosoma cruzi
Transcrição Genética
Trypanosoma
Expressão Gênica
description Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2018-11-14T17:05:51Z
dc.date.available.fl_str_mv 2018-11-14T17:05:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SERPELONI, Mariana et al. An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway. PLoS ONE, v. 6, n. 6, p. 1-14, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30033
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0020730
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv SERPELONI, Mariana et al. An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway. PLoS ONE, v. 6, n. 6, p. 1-14, 2011.
1932-6203
10.1371/journal.pone.0020730
url https://www.arca.fiocruz.br/handle/icict/30033
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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