Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.

Detalhes bibliográficos
Autor(a) principal: Oliveira, Camila Indiani de
Data de Publicação: 2004
Outros Autores: Teixeira, Maria Jânia, Teixeira, Clarissa Romero, Jesus, Joílson Ramos de, Rosato, Andréa Bomura, Silva, João Santana da, Brodskyn, Claudia Ida, Barral Netto, Manoel, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3264
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.
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spelling Oliveira, Camila Indiani deTeixeira, Maria JâniaTeixeira, Clarissa RomeroJesus, Joílson Ramos deRosato, Andréa BomuraSilva, João Santana daBrodskyn, Claudia IdaBarral Netto, ManoelBarral, Aldina Maria Prado2011-10-18T20:41:18Z2011-10-18T20:41:18Z2004OLIVEIRA, Camila Indiani de et al. Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice. Microbes Infection, v. 6, n. 11, p. 977-984, Sept. 2004.1286-4579https://www.arca.fiocruz.br/handle/icict/326410.1016/j.micinf.2004.05.009engLeishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.Leishmania braziliensis is the species responsible for the majority of cases of human cutaneous leishmaniasis in Brazil. In the present study, L. braziliensis isolates from two different geographic areas in Brazil were studied by RAPD, using arbitrary primers.We also evaluated other biological features of these two isolates. We compared (a) the clinical features they initiate or not once delivered subcutaneously as stationary-phase promastigotes in the footpad of BALB/c mice; (b) the parasite load in both the footpad and the draining lymph node; (c) the cytokines present in the supernatant of cultures of the cell suspensions from the draining lymph nodes; and (d) the cell types present at the site of parasite delivery. The results show that the L. braziliensis strain from Ceará (H3227) is genotypically different from the L. braziliensis strain from Bahia (BA788). H3227-parasitized mice developed detectable lesions, whereas BA788-parasitized mice did not. Fifteen days post parasite inoculation there was an increase in the numbers of macrophages and lymphocytes in the footpads, whatever the parasite inoculum. Parasite load at the inoculation site—namely the footpad—did not differ significantly; in draining lymph nodes, however, it increased over the period under study. Early after parasite inoculation, the cells recovered from the draining lymph nodes of BA788-parasitized mice produced higher levels of IFN-c, a feature coupled to a higher number of NK cells. Later, after the parasite inoculation, there was an increased content of IL-12p70 and IL-10 in the supernatant of cells recovered from the lymph nodes of H3227-parasitized mice. This comparative analysis points out that L. braziliensis isolates differing inLeishmania braziliensisRandom amplification of polymorphic DNAInterferon gammaInterleukin-12p70Interleukin-10Células CultivadasCitocinasImpressões Digitais de DNADNA de ProtozoárioModelos Animais de DoençasGenoma de ProtozoárioInterferon gamaInterleucina 10Interleucina-12Celulas Matadoras NaturaisLeishmania braziliensisLeishmaniose CutâneaLinfonodosCamundongos Endogâmicos BALB CPolimorfismo GenéticoSubunidades ProteícasTécnica de Amplificação ao Acaso de DNA Polimórficoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALIndiani de Oliveira C Leishmania braziliensis isolates....pdfIndiani de Oliveira C Leishmania braziliensis isolates....pdfapplication/pdf351462https://www.arca.fiocruz.br/bitstream/icict/3264/1/Indiani%20de%20Oliveira%20C%20Leishmania%20braziliensis%20isolates....pdf98f847aaacee43b6258851796bf4c43bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3264/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTIndiani de Oliveira C Leishmania braziliensis isolates....pdf.txtIndiani de Oliveira C Leishmania braziliensis isolates....pdf.txtExtracted texttext/plain35877https://www.arca.fiocruz.br/bitstream/icict/3264/5/Indiani%20de%20Oliveira%20C%20Leishmania%20braziliensis%20isolates....pdf.txtcdd0fd66149726a0ee890759161191fcMD55THUMBNAILIndiani de Oliveira C Leishmania braziliensis isolates....pdf.jpgIndiani de Oliveira C Leishmania braziliensis isolates....pdf.jpgGenerated Thumbnailimage/jpeg1746https://www.arca.fiocruz.br/bitstream/icict/3264/4/Indiani%20de%20Oliveira%20C%20Leishmania%20braziliensis%20isolates....pdf.jpg4121fe59a50e05d027197349c59f324cMD54icict/32642023-03-15 14:33:27.291oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
title Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
spellingShingle Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
Oliveira, Camila Indiani de
Leishmania braziliensis
Random amplification of polymorphic DNA
Interferon gamma
Interleukin-12p70
Interleukin-10
Células Cultivadas
Citocinas
Impressões Digitais de DNA
DNA de Protozoário
Modelos Animais de Doenças
Genoma de Protozoário
Interferon gama
Interleucina 10
Interleucina-12
Celulas Matadoras Naturais
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos
Camundongos Endogâmicos BALB C
Polimorfismo Genético
Subunidades Proteícas
Técnica de Amplificação ao Acaso de DNA Polimórfico
title_short Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
title_full Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
title_fullStr Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
title_full_unstemmed Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
title_sort Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice.
author Oliveira, Camila Indiani de
author_facet Oliveira, Camila Indiani de
Teixeira, Maria Jânia
Teixeira, Clarissa Romero
Jesus, Joílson Ramos de
Rosato, Andréa Bomura
Silva, João Santana da
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
author_role author
author2 Teixeira, Maria Jânia
Teixeira, Clarissa Romero
Jesus, Joílson Ramos de
Rosato, Andréa Bomura
Silva, João Santana da
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Camila Indiani de
Teixeira, Maria Jânia
Teixeira, Clarissa Romero
Jesus, Joílson Ramos de
Rosato, Andréa Bomura
Silva, João Santana da
Brodskyn, Claudia Ida
Barral Netto, Manoel
Barral, Aldina Maria Prado
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Random amplification of polymorphic DNA
Interferon gamma
Interleukin-12p70
Interleukin-10
topic Leishmania braziliensis
Random amplification of polymorphic DNA
Interferon gamma
Interleukin-12p70
Interleukin-10
Células Cultivadas
Citocinas
Impressões Digitais de DNA
DNA de Protozoário
Modelos Animais de Doenças
Genoma de Protozoário
Interferon gama
Interleucina 10
Interleucina-12
Celulas Matadoras Naturais
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos
Camundongos Endogâmicos BALB C
Polimorfismo Genético
Subunidades Proteícas
Técnica de Amplificação ao Acaso de DNA Polimórfico
dc.subject.decs.pt_BR.fl_str_mv Células Cultivadas
Citocinas
Impressões Digitais de DNA
DNA de Protozoário
Modelos Animais de Doenças
Genoma de Protozoário
Interferon gama
Interleucina 10
Interleucina-12
Celulas Matadoras Naturais
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos
Camundongos Endogâmicos BALB C
Polimorfismo Genético
Subunidades Proteícas
Técnica de Amplificação ao Acaso de DNA Polimórfico
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2011-10-18T20:41:18Z
dc.date.available.fl_str_mv 2011-10-18T20:41:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Camila Indiani de et al. Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice. Microbes Infection, v. 6, n. 11, p. 977-984, Sept. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3264
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv 10.1016/j.micinf.2004.05.009
identifier_str_mv OLIVEIRA, Camila Indiani de et al. Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice. Microbes Infection, v. 6, n. 11, p. 977-984, Sept. 2004.
1286-4579
10.1016/j.micinf.2004.05.009
url https://www.arca.fiocruz.br/handle/icict/3264
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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